Biological control of stem rot of groundnut (Arachis hypogaea L.) caused by Sclerotium rolfsii Sacc. with actinomycetes

M. Adhilakshmi, P. Latha, V. Paranidharan, D. Balachandar, K. Ganesamurthy, R. Velazhahan

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A total of 30 isolates of actinomycetes were isolated from the rhizosphere soils of groundnut collected from different parts of Tamil Nadu, India and tested for their inhibitory activity against Sclerotium rolfsii Sacc., the stem rot pathogen of groundnut. Among the various isolates tested in vitro, five isolates (CBE, MDU, PDK, ANR and SA) were found effective in inhibiting the mycelial growth of S. rolfsii in dual culture assay. These isolates were identified as Streptomyces sp. on the basis of standard bacteriological tests and 16S ribosomal RNA gene sequence analysis. Groundnut seeds when treated with Streptomyces sp. strains showed significant increases in root length, shoot length and seedling vigour. Talc-based powder formulations of the Streptomyces sp. strains, CBE, MDU and PDK selected on the basis of their in vitro antagonism on dual plate technique and plant-growth promoting activity were prepared and tested for their efficacy in controlling stem rot of groundnut under greenhouse and field conditions. Seed treatment and soil application of Streptomyces sp. strains CBE, MDU and PDK significantly reduced the incidence of stem rot under greenhouse and field conditions. The highest pod yield was recorded in plots treated with Streptomyces sp. strain CBE when compared to other strains.

Original languageEnglish
Pages (from-to)298-311
Number of pages14
JournalArchives of Phytopathology and Plant Protection
Volume47
Issue number3
DOIs
Publication statusPublished - Feb 2014

Fingerprint

Athelia rolfsii
stem rot
Actinobacteria
Streptomyces
Arachis hypogaea
peanuts
biological control
greenhouses
talc
India
seed treatment
soil treatment
vigor
pods
powders
rhizosphere
sequence analysis
ribosomal RNA
plant growth
nucleotide sequences

Keywords

  • actinomycetes
  • Arachis hypogaea
  • biological control
  • Sclerotium rolfsii
  • stem rot

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Biological control of stem rot of groundnut (Arachis hypogaea L.) caused by Sclerotium rolfsii Sacc. with actinomycetes. / Adhilakshmi, M.; Latha, P.; Paranidharan, V.; Balachandar, D.; Ganesamurthy, K.; Velazhahan, R.

In: Archives of Phytopathology and Plant Protection, Vol. 47, No. 3, 02.2014, p. 298-311.

Research output: Contribution to journalArticle

Adhilakshmi, M. ; Latha, P. ; Paranidharan, V. ; Balachandar, D. ; Ganesamurthy, K. ; Velazhahan, R. / Biological control of stem rot of groundnut (Arachis hypogaea L.) caused by Sclerotium rolfsii Sacc. with actinomycetes. In: Archives of Phytopathology and Plant Protection. 2014 ; Vol. 47, No. 3. pp. 298-311.
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