Biodegradation of partially hydrolyzed polyacrylamide hpam using bacteria isolated from omani oil fields

Walaa Al-Moqbali, Sanket J. Joshi, Saif Al-Bahry, Yahya Al-Wahaibi, Abdulkadir Elshafie, H.E. Dr. Ali AL-Bimani, Abdul-Aziz M. Al-Hashmi, Sathish Babu Soundra Pandian

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

In present study the biodegradation of partially hydrolyzed polyacrylamide (HPAM) by bacterial strains isolated from Omani oil fields was analyzed. HPAMs are extensively used in oil fields for enhanced oil recovery operations. The produced water after polymer flooding poses grave ecological problems, such as it could raise the difficulty of oil–water separation, producing toxic acrylamide that degrades naturally, which threatens the local environment. Biodegradation of HPAM by microbes may be an efficient way to solve those problems. Microbial biodegradation is considered an environmentally friendly safe technique. The isolation of microbes that are able to degrade HPAM from the oil field produced water was investigated in this study. The bacterial isolates were identified by MALDI-Biotyper and the biodegradation of HPAM was analyzed by LC-MS and reduction in viscosity by rheometer. Two HPAM degrading bacterial strains Bacillus sp. (NP-10) and Chelatococcus sp. (NP-11) were isolated from the polymer-flooding produced water. The removal efficiency of HPAM based on shear viscosity for NP10 andNP11 were 59.9% and 58.4%, respectively after 9-days treatment period, at pH 7.1, 40 °C and 160 rpm. The addition of glucose (0.5 g/l) did not improve the HPAM degradation for NP11. However, the degradation percentage by NP10 reached to 68.8%. HPAM samples after bacterial biodegradation were analyzed by LC-MS. The results showed the presence of acrylamide monomers in the culture after biodegradation. Three other isolated strains were identified as NP-9, NP-2 and NP-8 were able to reduce the concentration of acrylamide significantly. Present findings proved that both Bacillus sp. (NP-10) and Chelatococcus sp. (NP-11) could be suitable candidates for biodegradation of HPAM and NP-9, NP-2 and NP-8 strains as potential candidates for reducing the acrylamide concentrations, as an environmental friendly approach.

Original languageEnglish
Title of host publicationSociety of Petroleum Engineers - SPE EOR Conference at Oil and Gas West Asia 2018
PublisherSociety of Petroleum Engineers
Volume2018-March
ISBN (Electronic)9781613995693
Publication statusPublished - Jan 1 2018
EventSPE EOR Conference at Oil and Gas West Asia, OGWA 2018 - Muscat, Oman
Duration: Mar 26 2018Mar 28 2018

Other

OtherSPE EOR Conference at Oil and Gas West Asia, OGWA 2018
CountryOman
CityMuscat
Period3/26/183/28/18

Fingerprint

Oil fields
Biodegradation
Polyacrylates
oil field
biodegradation
Bacteria
bacterium
Acrylamide
Bacilli
viscosity
flooding
polymer
Polymers
degradation
enhanced oil recovery
polyacrylamide
Degradation
water
Shear viscosity
Poisons

ASJC Scopus subject areas

  • Fuel Technology
  • Energy Engineering and Power Technology
  • Geochemistry and Petrology

Cite this

Al-Moqbali, W., Joshi, S. J., Al-Bahry, S., Al-Wahaibi, Y., Elshafie, A., AL-Bimani, H. E. D. A., ... Soundra Pandian, S. B. (2018). Biodegradation of partially hydrolyzed polyacrylamide hpam using bacteria isolated from omani oil fields. In Society of Petroleum Engineers - SPE EOR Conference at Oil and Gas West Asia 2018 (Vol. 2018-March). Society of Petroleum Engineers.

Biodegradation of partially hydrolyzed polyacrylamide hpam using bacteria isolated from omani oil fields. / Al-Moqbali, Walaa; Joshi, Sanket J.; Al-Bahry, Saif; Al-Wahaibi, Yahya; Elshafie, Abdulkadir; AL-Bimani, H.E. Dr. Ali; M. Al-Hashmi, Abdul-Aziz; Soundra Pandian, Sathish Babu.

Society of Petroleum Engineers - SPE EOR Conference at Oil and Gas West Asia 2018. Vol. 2018-March Society of Petroleum Engineers, 2018.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Al-Moqbali, W, Joshi, SJ, Al-Bahry, S, Al-Wahaibi, Y, Elshafie, A, AL-Bimani, HEDA, M. Al-Hashmi, A-A & Soundra Pandian, SB 2018, Biodegradation of partially hydrolyzed polyacrylamide hpam using bacteria isolated from omani oil fields. in Society of Petroleum Engineers - SPE EOR Conference at Oil and Gas West Asia 2018. vol. 2018-March, Society of Petroleum Engineers, SPE EOR Conference at Oil and Gas West Asia, OGWA 2018, Muscat, Oman, 3/26/18.
Al-Moqbali W, Joshi SJ, Al-Bahry S, Al-Wahaibi Y, Elshafie A, AL-Bimani HEDA et al. Biodegradation of partially hydrolyzed polyacrylamide hpam using bacteria isolated from omani oil fields. In Society of Petroleum Engineers - SPE EOR Conference at Oil and Gas West Asia 2018. Vol. 2018-March. Society of Petroleum Engineers. 2018
Al-Moqbali, Walaa ; Joshi, Sanket J. ; Al-Bahry, Saif ; Al-Wahaibi, Yahya ; Elshafie, Abdulkadir ; AL-Bimani, H.E. Dr. Ali ; M. Al-Hashmi, Abdul-Aziz ; Soundra Pandian, Sathish Babu. / Biodegradation of partially hydrolyzed polyacrylamide hpam using bacteria isolated from omani oil fields. Society of Petroleum Engineers - SPE EOR Conference at Oil and Gas West Asia 2018. Vol. 2018-March Society of Petroleum Engineers, 2018.
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abstract = "In present study the biodegradation of partially hydrolyzed polyacrylamide (HPAM) by bacterial strains isolated from Omani oil fields was analyzed. HPAMs are extensively used in oil fields for enhanced oil recovery operations. The produced water after polymer flooding poses grave ecological problems, such as it could raise the difficulty of oil–water separation, producing toxic acrylamide that degrades naturally, which threatens the local environment. Biodegradation of HPAM by microbes may be an efficient way to solve those problems. Microbial biodegradation is considered an environmentally friendly safe technique. The isolation of microbes that are able to degrade HPAM from the oil field produced water was investigated in this study. The bacterial isolates were identified by MALDI-Biotyper and the biodegradation of HPAM was analyzed by LC-MS and reduction in viscosity by rheometer. Two HPAM degrading bacterial strains Bacillus sp. (NP-10) and Chelatococcus sp. (NP-11) were isolated from the polymer-flooding produced water. The removal efficiency of HPAM based on shear viscosity for NP10 andNP11 were 59.9{\%} and 58.4{\%}, respectively after 9-days treatment period, at pH 7.1, 40 °C and 160 rpm. The addition of glucose (0.5 g/l) did not improve the HPAM degradation for NP11. However, the degradation percentage by NP10 reached to 68.8{\%}. HPAM samples after bacterial biodegradation were analyzed by LC-MS. The results showed the presence of acrylamide monomers in the culture after biodegradation. Three other isolated strains were identified as NP-9, NP-2 and NP-8 were able to reduce the concentration of acrylamide significantly. Present findings proved that both Bacillus sp. (NP-10) and Chelatococcus sp. (NP-11) could be suitable candidates for biodegradation of HPAM and NP-9, NP-2 and NP-8 strains as potential candidates for reducing the acrylamide concentrations, as an environmental friendly approach.",
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AU - Al-Wahaibi, Yahya

AU - Elshafie, Abdulkadir

AU - AL-Bimani, H.E. Dr. Ali

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