Biochemical characterization of riboflavin carrier protein (RCP) in prostate cancer

Tanya Johnson, Allal Ouhtit, Rajiv Gaur, Augusta Fernando, Paul Schwarzenberger, Joseph Su, Mohamed F. Ismail, Hassan I. El-Sayyad, Anjali Karande, Zakaria Abd Elmageed, Prakash Rao, Madhwa Raj

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Riboflavin carrier protein (RCP) is a growth-and development-specific protein. Here, we characterized the expression of this protein in prostate cancer by polyclonal and monoclonal antibodies against chicken RCP. RCP was localized to both androgen-dependent and independent prostate cancer cell lines. Compared to controls, RCP was overexpressed in all 45 prostate adenocarcinomas, irrespective of the Gleason's score or the stage of the disease. The identified RCP had a molecular weight of 38 kDa, similar to RCP purified from chicken. Presence of this protein was also confirmed by siRNA inhibition analysis. Antibodies to chicken RCP inhibited incorporation of tritiated thymidine into DNA and prevented riboflavin uptake in PC3 prostate cancer cells, suggesting a critical function of this protein in prostate cancer cell growth. These data suggest that RCP can be used as a tumor biomarker in prostate cancer.

Original languageEnglish
Pages (from-to)3634-3640
Number of pages7
JournalFrontiers in Bioscience
Volume14
Issue number10
DOIs
Publication statusPublished - Jan 1 2009

Keywords

  • Antibody
  • Prostate cancer
  • Riboflavin carrier protein
  • Tumor marker

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)

Fingerprint Dive into the research topics of 'Biochemical characterization of riboflavin carrier protein (RCP) in prostate cancer'. Together they form a unique fingerprint.

  • Cite this

    Johnson, T., Ouhtit, A., Gaur, R., Fernando, A., Schwarzenberger, P., Su, J., Ismail, M. F., El-Sayyad, H. I., Karande, A., Elmageed, Z. A., Rao, P., & Raj, M. (2009). Biochemical characterization of riboflavin carrier protein (RCP) in prostate cancer. Frontiers in Bioscience, 14(10), 3634-3640. https://doi.org/10.2735/3477