TY - JOUR
T1 - Biochemical characterization of riboflavin carrier protein (RCP) in prostate cancer
AU - Johnson, Tanya
AU - Ouhtit, Allal
AU - Gaur, Rajiv
AU - Fernando, Augusta
AU - Schwarzenberger, Paul
AU - Su, Joseph
AU - Ismail, Mohamed F.
AU - El-Sayyad, Hassan I.
AU - Karande, Anjali
AU - Elmageed, Zakaria Abd
AU - Rao, Prakash
AU - Raj, Madhwa
PY - 2009/1/1
Y1 - 2009/1/1
N2 - Riboflavin carrier protein (RCP) is a growth-and development-specific protein. Here, we characterized the expression of this protein in prostate cancer by polyclonal and monoclonal antibodies against chicken RCP. RCP was localized to both androgen-dependent and independent prostate cancer cell lines. Compared to controls, RCP was overexpressed in all 45 prostate adenocarcinomas, irrespective of the Gleason's score or the stage of the disease. The identified RCP had a molecular weight of 38 kDa, similar to RCP purified from chicken. Presence of this protein was also confirmed by siRNA inhibition analysis. Antibodies to chicken RCP inhibited incorporation of tritiated thymidine into DNA and prevented riboflavin uptake in PC3 prostate cancer cells, suggesting a critical function of this protein in prostate cancer cell growth. These data suggest that RCP can be used as a tumor biomarker in prostate cancer.
AB - Riboflavin carrier protein (RCP) is a growth-and development-specific protein. Here, we characterized the expression of this protein in prostate cancer by polyclonal and monoclonal antibodies against chicken RCP. RCP was localized to both androgen-dependent and independent prostate cancer cell lines. Compared to controls, RCP was overexpressed in all 45 prostate adenocarcinomas, irrespective of the Gleason's score or the stage of the disease. The identified RCP had a molecular weight of 38 kDa, similar to RCP purified from chicken. Presence of this protein was also confirmed by siRNA inhibition analysis. Antibodies to chicken RCP inhibited incorporation of tritiated thymidine into DNA and prevented riboflavin uptake in PC3 prostate cancer cells, suggesting a critical function of this protein in prostate cancer cell growth. These data suggest that RCP can be used as a tumor biomarker in prostate cancer.
KW - Antibody
KW - Prostate cancer
KW - Riboflavin carrier protein
KW - Tumor marker
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U2 - 10.2735/3477
DO - 10.2735/3477
M3 - Article
C2 - 19273299
AN - SCOPUS:63849152791
SN - 2768-6701
VL - 14
SP - 3634
EP - 3640
JO - Frontiers in Bioscience
JF - Frontiers in Bioscience
IS - 10
ER -
