Biochemical characterization of riboflavin carrier protein (RCP) in prostate cancer

Tanya Johnson, Allal Ouhtit, Rajiv Gaur, Augusta Fernando, Paul Schwarzenberger, Joseph Su, Mohamed F. Ismail, Hassan I. El-Sayyad, Anjali Karande, Zakaria Abd Elmageed, Prakash Rao, Madhwa Raj

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Riboflavin carrier protein (RCP) is a growth-and development-specific protein. Here, we characterized the expression of this protein in prostate cancer by polyclonal and monoclonal antibodies against chicken RCP. RCP was localized to both androgen-dependent and independent prostate cancer cell lines. Compared to controls, RCP was overexpressed in all 45 prostate adenocarcinomas, irrespective of the Gleason's score or the stage of the disease. The identified RCP had a molecular weight of 38 kDa, similar to RCP purified from chicken. Presence of this protein was also confirmed by siRNA inhibition analysis. Antibodies to chicken RCP inhibited incorporation of tritiated thymidine into DNA and prevented riboflavin uptake in PC3 prostate cancer cells, suggesting a critical function of this protein in prostate cancer cell growth. These data suggest that RCP can be used as a tumor biomarker in prostate cancer.

Original languageEnglish
Pages (from-to)3634-3640
Number of pages7
JournalFrontiers in Bioscience
Volume14
Issue number10
DOIs
Publication statusPublished - Jan 1 2009

Fingerprint

Prostatic Neoplasms
Chickens
Proteins
Cells
riboflavin-binding protein
Riboflavin
Neoplasm Grading
Cell growth
Tumor Biomarkers
Growth and Development
Thymidine
Small Interfering RNA
Androgens
Prostate
Adenocarcinoma
Molecular Weight
Molecular weight
Monoclonal Antibodies
Cell Line
Antibodies

Keywords

  • Antibody
  • Prostate cancer
  • Riboflavin carrier protein
  • Tumor marker

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)

Cite this

Johnson, T., Ouhtit, A., Gaur, R., Fernando, A., Schwarzenberger, P., Su, J., ... Raj, M. (2009). Biochemical characterization of riboflavin carrier protein (RCP) in prostate cancer. Frontiers in Bioscience, 14(10), 3634-3640. https://doi.org/10.2735/3477

Biochemical characterization of riboflavin carrier protein (RCP) in prostate cancer. / Johnson, Tanya; Ouhtit, Allal; Gaur, Rajiv; Fernando, Augusta; Schwarzenberger, Paul; Su, Joseph; Ismail, Mohamed F.; El-Sayyad, Hassan I.; Karande, Anjali; Elmageed, Zakaria Abd; Rao, Prakash; Raj, Madhwa.

In: Frontiers in Bioscience, Vol. 14, No. 10, 01.01.2009, p. 3634-3640.

Research output: Contribution to journalArticle

Johnson, T, Ouhtit, A, Gaur, R, Fernando, A, Schwarzenberger, P, Su, J, Ismail, MF, El-Sayyad, HI, Karande, A, Elmageed, ZA, Rao, P & Raj, M 2009, 'Biochemical characterization of riboflavin carrier protein (RCP) in prostate cancer', Frontiers in Bioscience, vol. 14, no. 10, pp. 3634-3640. https://doi.org/10.2735/3477
Johnson T, Ouhtit A, Gaur R, Fernando A, Schwarzenberger P, Su J et al. Biochemical characterization of riboflavin carrier protein (RCP) in prostate cancer. Frontiers in Bioscience. 2009 Jan 1;14(10):3634-3640. https://doi.org/10.2735/3477
Johnson, Tanya ; Ouhtit, Allal ; Gaur, Rajiv ; Fernando, Augusta ; Schwarzenberger, Paul ; Su, Joseph ; Ismail, Mohamed F. ; El-Sayyad, Hassan I. ; Karande, Anjali ; Elmageed, Zakaria Abd ; Rao, Prakash ; Raj, Madhwa. / Biochemical characterization of riboflavin carrier protein (RCP) in prostate cancer. In: Frontiers in Bioscience. 2009 ; Vol. 14, No. 10. pp. 3634-3640.
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