AtMBD9 modulates Arabidopsis development through the dual epigenetic pathways of DNA methylation and histone acetylation

M. W F Yaish, Mingsheng Peng, Steven J. Rothstein

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Mutations within the Arabidopsis METHYL-CpG BINDING DOMAIN 9 gene (AtMBD9) cause pleotropic phenotypes including early flowering and multiple lateral branches. Early flowering was previously attributed to the repression of flowering locus C (FLC) due to a reduction in histone acetylation. However, the reasons for other phenotypic variations remained obscure. Recent studies suggest an important functional correlation between DNA methylation and histone modifications. By investigating this relationship, we found that the global genomic DNA of atmbd9 was over-methylated, including the FLC gene region. Recombinant AtMBD9 does not have detectable DNA demethylation activity in vitro, but instead has histone acetylation activity. Ectopic over-expression of AtMBD9 and transient DNA demethylation promotes flowering and causes partial recovery of the normal branching phenotype. Co-immunoprecipitation assays suggest that AtMBD9 interacts in vivo with some regions of the FLC gene and binds to histone 4 (H4). Gene expression profile analysis revealed earlier up-regulation of some flower-specific transcriptional factors and alteration of potential hormonal and signal transducer axillary branching regulatory genes. In accordance with this result, AtMBD9 itself was found to be localized in the nucleus and expressed in the flower and axillary buds. Together, these results suggest that AtMBD9 controls flowering time and axillary branching by modulating gene expression through DNA methylation and histone acetylation, and reveal another component of the epigenetic mechanism controlling gene expression.

Original languageEnglish
Pages (from-to)123-135
Number of pages13
JournalPlant Journal
Volume59
Issue number1
DOIs
Publication statusPublished - Jul 2009

Fingerprint

acetylation
DNA methylation
DNA Methylation
Acetylation
Arabidopsis
Epigenomics
histones
epigenetics
Histones
flowering
DNA
Histone Code
branching
Genes
Phenotype
Gene Expression
gene expression
Regulator Genes
loci
Transducers

Keywords

  • Acetylation
  • Arabidopsis
  • At MBD9
  • Epigenetics
  • Histone
  • Methylation

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Genetics

Cite this

AtMBD9 modulates Arabidopsis development through the dual epigenetic pathways of DNA methylation and histone acetylation. / Yaish, M. W F; Peng, Mingsheng; Rothstein, Steven J.

In: Plant Journal, Vol. 59, No. 1, 07.2009, p. 123-135.

Research output: Contribution to journalArticle

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