Association of an alphasatellite with tomato yellow leaf curl virus and ageratum yellow vein virus in Japan is suggestive of a recent introduction

Muhammad Shafiq Shahid, Masato Ikegami, Abdul Waheed, Rob W. Briddon, Keiko T. Natsuaki

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Samples were collected in 2011 from tomato plants exhibiting typical tomato leaf curl disease symptoms in the vicinity of Komae, Japan. PCR mediated amplification, cloning and sequencing of all begomovirus components from two plants from different fields showed the plants to be infected by Tomato yellow leaf curl virus (TYLCV) and Ageratum yellow vein virus (AYVV). Both viruses have previously been shown to be present in Japan, although this is the first identification of AYVV on mainland Japan; the virus previously having been shown to be present on the Okinawa Islands. The plant harboring AYVV was also shown to contain the betasatellite Tomato leaf curl Java betasatellite (ToLCJaB), a satellite not previously shown to be present in Japan. No betasatellite was associated with the TYLCV infected tomato plants analyzed here, consistent with earlier findings for this virus in Japan. Surprisingly both plants were also found to harbor an alphasatellite; no alphasatellites having previously been reported from Japan. The alphasatellite associated with both viruses was shown to be Sida yellow vein China alphasatellite which has previously only been identified in the Yunnan Province of China and Nepal. The results suggest that further begomoviruses, and their associated satellites, are being introduced to Japan. The significance of these findings is discussed.

Original languageEnglish
Pages (from-to)189-200
Number of pages12
JournalViruses
Volume6
Issue number1
DOIs
Publication statusPublished - Jan 14 2014

Fingerprint

Ageratum
Lycopersicon esculentum
Veins
Japan
Viruses
Begomovirus
China
Plant Structures
Nepal
Islands
Organism Cloning

Keywords

  • Alphasatellite
  • Begomovirus
  • Betasatellite
  • Geminivirus
  • Tomato

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Association of an alphasatellite with tomato yellow leaf curl virus and ageratum yellow vein virus in Japan is suggestive of a recent introduction. / Shahid, Muhammad Shafiq; Ikegami, Masato; Waheed, Abdul; Briddon, Rob W.; Natsuaki, Keiko T.

In: Viruses, Vol. 6, No. 1, 14.01.2014, p. 189-200.

Research output: Contribution to journalArticle

Shahid, Muhammad Shafiq ; Ikegami, Masato ; Waheed, Abdul ; Briddon, Rob W. ; Natsuaki, Keiko T. / Association of an alphasatellite with tomato yellow leaf curl virus and ageratum yellow vein virus in Japan is suggestive of a recent introduction. In: Viruses. 2014 ; Vol. 6, No. 1. pp. 189-200.
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