Analysis of incensole acetate in Boswellia species by near infrared spectroscopy coupled with partial least squares regression and cross-validation by high-performance liquid chromatography

Najeeb Ur Rehman, Sulaiman Al-Shidhani, Ahmed Al-Harrasi*, Ahmed Al-Rawahi, Fazal Mabood, Muhammed Al-Broumi, Mohammed Al-Azri, Tanveer Alam, Javid Hussain

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Incensole acetate (diterpenoid), macrocyclic cembrenoid, found in significant amounts in Boswellia papyrifera has been shown to cause anti-inflammatory, psychoactive, anxiolytic and anti-depressive effects. Boswellia is the main source of incensole acetate and therefore developing a reliable and fast method of its analysis is of high importance. Thin layer chromatography and near infrared spectroscopy coupled with partial least squares regression were used for a rapid quantification of incensole acetate in the resins of five Boswellia species. The findings showed that the acetylated fraction of Boswellia papyrifera resin (MeOH extract) yielded the maximum amount of incensole acetate (32.87%) followed by n-hexane (14.66%) and ethyl acetate (2.84%), while no incensole acetate was found in the resins from four other species. The results obtained by non-destructive NIR spectroscopy are in complete agreement with the results obtained by HPLC, suggesting that the two alternative methods provide fast quantitative analysis of incensole acetate in B. papyrifera.

Original languageEnglish
Pages (from-to)18-24
Number of pages7
JournalJournal of Near Infrared Spectroscopy
Issue number1
Publication statusPublished - Feb 1 2020
Externally publishedYes


  • Boswellia papyrifera
  • HPLC analysis
  • Incensole acetate
  • Incensole acetate
  • NIR spectroscopy
  • PLS regression

ASJC Scopus subject areas

  • Spectroscopy

Cite this