Amorphous silica nanoparticles impair vascular homeostasis and induce systemic inflammation

Abderrahim Nemmar, Sulayma Albarwani, Sumaya Beegam, Priya Yuvaraju, Javed Yasin, Samir Attoub, Badreldin H. Ali

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Amorphous silica nanoparticles (SiNPs) are being used in biomedical, pharmaceutical, and many other industrial applications entailing human exposure. However, their potential vascular and systemic pathophysiologic effects are not fully understood. Here, we investigated the acute (24 hours) systemic toxicity of intraperitoneally administered 50 nm and 500 nm SiNPs in mice (0.5 mg/kg). Both sizes of SiNPs induced a platelet proaggregatory effect in pial venules and increased plasma concentration of plasminogen activator inhibitor-1. Elevated plasma levels of von Willebrand factor and fibrinogen and a decrease in the number of circulating platelets were only seen following the administration of 50 nm SiNPs. The direct addition of SiNPs to untreated mouse blood significantly induced in vitro platelet aggregation in a dose-dependent fashion, and these effects were more pronounced with 50 nm SiNPs. Both sizes of SiNPs increased lactate dehydrogenase activity and interleukin 1β concentration. However, tumor necrosis factor α concentration was only increased after the administration of 50 nm SiNPs. Nevertheless, plasma markers of oxidative stress, including 8-isoprostane, thiobarbituric acid reactive substances, catalase, and glutathione S-transferase, were not affected by SiNPs. The in vitro exposure of human umbilical vein endothelial cells to SiNPs showed a reduced cellular viability, and more potency was seen with 50 nm SiNPs. Both sizes of SiNPs caused a decrease in endothelium-dependent relaxation of isolated small mesenteric arteries. We conclude that amorphous SiNPs cause systemic inflammation and coagulation events, and alter vascular reactivity. Overall, the effects observed with 50 nm SiNPs were more pronounced than those with 500 nm SiNPs. These findings provide new insight into the deleterious effect of amorphous SiNPs on vascular homeostasis.

Original languageEnglish
Pages (from-to)2279-2789
Number of pages511
JournalInternational Journal of Nanomedicine
Volume9
Issue number1
DOIs
Publication statusPublished - Jun 2 2014

Fingerprint

Silicon Dioxide
Nanoparticles
Blood Vessels
Homeostasis
Silica
Inflammation
Platelets
8-epi-prostaglandin F2alpha
Plasmas
Mesenteric Arteries
Oxidative stress
Venules
Thiobarbituric Acid Reactive Substances
Plasminogen Activator Inhibitor 1
Endothelial cells
Human Umbilical Vein Endothelial Cells
von Willebrand Factor
Coagulation
Glutathione Transferase
Platelet Count

Keywords

  • Amorphous silica nanoparticles
  • Systemic inflammation
  • Thrombosis
  • Toxicity

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Organic Chemistry
  • Drug Discovery
  • Medicine(all)

Cite this

Amorphous silica nanoparticles impair vascular homeostasis and induce systemic inflammation. / Nemmar, Abderrahim; Albarwani, Sulayma; Beegam, Sumaya; Yuvaraju, Priya; Yasin, Javed; Attoub, Samir; Ali, Badreldin H.

In: International Journal of Nanomedicine, Vol. 9, No. 1, 02.06.2014, p. 2279-2789.

Research output: Contribution to journalArticle

Nemmar, Abderrahim ; Albarwani, Sulayma ; Beegam, Sumaya ; Yuvaraju, Priya ; Yasin, Javed ; Attoub, Samir ; Ali, Badreldin H. / Amorphous silica nanoparticles impair vascular homeostasis and induce systemic inflammation. In: International Journal of Nanomedicine. 2014 ; Vol. 9, No. 1. pp. 2279-2789.
@article{86737ce496c54a4193f9cee2727c0807,
title = "Amorphous silica nanoparticles impair vascular homeostasis and induce systemic inflammation",
abstract = "Amorphous silica nanoparticles (SiNPs) are being used in biomedical, pharmaceutical, and many other industrial applications entailing human exposure. However, their potential vascular and systemic pathophysiologic effects are not fully understood. Here, we investigated the acute (24 hours) systemic toxicity of intraperitoneally administered 50 nm and 500 nm SiNPs in mice (0.5 mg/kg). Both sizes of SiNPs induced a platelet proaggregatory effect in pial venules and increased plasma concentration of plasminogen activator inhibitor-1. Elevated plasma levels of von Willebrand factor and fibrinogen and a decrease in the number of circulating platelets were only seen following the administration of 50 nm SiNPs. The direct addition of SiNPs to untreated mouse blood significantly induced in vitro platelet aggregation in a dose-dependent fashion, and these effects were more pronounced with 50 nm SiNPs. Both sizes of SiNPs increased lactate dehydrogenase activity and interleukin 1β concentration. However, tumor necrosis factor α concentration was only increased after the administration of 50 nm SiNPs. Nevertheless, plasma markers of oxidative stress, including 8-isoprostane, thiobarbituric acid reactive substances, catalase, and glutathione S-transferase, were not affected by SiNPs. The in vitro exposure of human umbilical vein endothelial cells to SiNPs showed a reduced cellular viability, and more potency was seen with 50 nm SiNPs. Both sizes of SiNPs caused a decrease in endothelium-dependent relaxation of isolated small mesenteric arteries. We conclude that amorphous SiNPs cause systemic inflammation and coagulation events, and alter vascular reactivity. Overall, the effects observed with 50 nm SiNPs were more pronounced than those with 500 nm SiNPs. These findings provide new insight into the deleterious effect of amorphous SiNPs on vascular homeostasis.",
keywords = "Amorphous silica nanoparticles, Systemic inflammation, Thrombosis, Toxicity",
author = "Abderrahim Nemmar and Sulayma Albarwani and Sumaya Beegam and Priya Yuvaraju and Javed Yasin and Samir Attoub and Ali, {Badreldin H.}",
year = "2014",
month = "6",
day = "2",
doi = "10.2147/IJN.S52818",
language = "English",
volume = "9",
pages = "2279--2789",
journal = "International Journal of Nanomedicine",
issn = "1176-9114",
publisher = "Dove Medical Press Ltd.",
number = "1",

}

TY - JOUR

T1 - Amorphous silica nanoparticles impair vascular homeostasis and induce systemic inflammation

AU - Nemmar, Abderrahim

AU - Albarwani, Sulayma

AU - Beegam, Sumaya

AU - Yuvaraju, Priya

AU - Yasin, Javed

AU - Attoub, Samir

AU - Ali, Badreldin H.

PY - 2014/6/2

Y1 - 2014/6/2

N2 - Amorphous silica nanoparticles (SiNPs) are being used in biomedical, pharmaceutical, and many other industrial applications entailing human exposure. However, their potential vascular and systemic pathophysiologic effects are not fully understood. Here, we investigated the acute (24 hours) systemic toxicity of intraperitoneally administered 50 nm and 500 nm SiNPs in mice (0.5 mg/kg). Both sizes of SiNPs induced a platelet proaggregatory effect in pial venules and increased plasma concentration of plasminogen activator inhibitor-1. Elevated plasma levels of von Willebrand factor and fibrinogen and a decrease in the number of circulating platelets were only seen following the administration of 50 nm SiNPs. The direct addition of SiNPs to untreated mouse blood significantly induced in vitro platelet aggregation in a dose-dependent fashion, and these effects were more pronounced with 50 nm SiNPs. Both sizes of SiNPs increased lactate dehydrogenase activity and interleukin 1β concentration. However, tumor necrosis factor α concentration was only increased after the administration of 50 nm SiNPs. Nevertheless, plasma markers of oxidative stress, including 8-isoprostane, thiobarbituric acid reactive substances, catalase, and glutathione S-transferase, were not affected by SiNPs. The in vitro exposure of human umbilical vein endothelial cells to SiNPs showed a reduced cellular viability, and more potency was seen with 50 nm SiNPs. Both sizes of SiNPs caused a decrease in endothelium-dependent relaxation of isolated small mesenteric arteries. We conclude that amorphous SiNPs cause systemic inflammation and coagulation events, and alter vascular reactivity. Overall, the effects observed with 50 nm SiNPs were more pronounced than those with 500 nm SiNPs. These findings provide new insight into the deleterious effect of amorphous SiNPs on vascular homeostasis.

AB - Amorphous silica nanoparticles (SiNPs) are being used in biomedical, pharmaceutical, and many other industrial applications entailing human exposure. However, their potential vascular and systemic pathophysiologic effects are not fully understood. Here, we investigated the acute (24 hours) systemic toxicity of intraperitoneally administered 50 nm and 500 nm SiNPs in mice (0.5 mg/kg). Both sizes of SiNPs induced a platelet proaggregatory effect in pial venules and increased plasma concentration of plasminogen activator inhibitor-1. Elevated plasma levels of von Willebrand factor and fibrinogen and a decrease in the number of circulating platelets were only seen following the administration of 50 nm SiNPs. The direct addition of SiNPs to untreated mouse blood significantly induced in vitro platelet aggregation in a dose-dependent fashion, and these effects were more pronounced with 50 nm SiNPs. Both sizes of SiNPs increased lactate dehydrogenase activity and interleukin 1β concentration. However, tumor necrosis factor α concentration was only increased after the administration of 50 nm SiNPs. Nevertheless, plasma markers of oxidative stress, including 8-isoprostane, thiobarbituric acid reactive substances, catalase, and glutathione S-transferase, were not affected by SiNPs. The in vitro exposure of human umbilical vein endothelial cells to SiNPs showed a reduced cellular viability, and more potency was seen with 50 nm SiNPs. Both sizes of SiNPs caused a decrease in endothelium-dependent relaxation of isolated small mesenteric arteries. We conclude that amorphous SiNPs cause systemic inflammation and coagulation events, and alter vascular reactivity. Overall, the effects observed with 50 nm SiNPs were more pronounced than those with 500 nm SiNPs. These findings provide new insight into the deleterious effect of amorphous SiNPs on vascular homeostasis.

KW - Amorphous silica nanoparticles

KW - Systemic inflammation

KW - Thrombosis

KW - Toxicity

UR - http://www.scopus.com/inward/record.url?scp=84901918642&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84901918642&partnerID=8YFLogxK

U2 - 10.2147/IJN.S52818

DO - 10.2147/IJN.S52818

M3 - Article

C2 - 24936130

AN - SCOPUS:84901918642

VL - 9

SP - 2279

EP - 2789

JO - International Journal of Nanomedicine

JF - International Journal of Nanomedicine

SN - 1176-9114

IS - 1

ER -