Airborne dust collected from Kuwait in 1991-1992 augments peroxidation of cellular membrane lipids and enhances DNA damage

Mohammad Athar, Mohammad Iqbal, Mirza U. Beg, Dhari Al-Ajmi, Saleh Al-Muzaini

Research output: Contribution to journalArticle

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Abstract

This paper reports the results of studies on airborne dust samples collected from the Ahmadi area of Kuwait in 1991, 1992, and 1995. These samples were tested for their ability to enhance peroxidative damage of lipid-rich biological membranes. The damage caused by the dust collected during 1991 and 1992 enhanced both enzymatic and non-enzymatic lipid peroxidation more significantly as compared to the dust collected during 1995. In a separate study, these samples were further tested for their ability to enhance DNA damage. The samples of 1991-92 caused single strand breaks as well as deoxyribose sugar damage. The enhancement in the single strand breaks was 33 to 40%. To find out the mechanism of DNA damage and lipid peroxidation, similar studies were performed in the presence of quenchers of hydroxyl radicals, superoxide anions (O2·), singlet oxygen and hydrogen peroxide (H2O2). The O2· scavenger, superoxide dismutase, and quencher of H2O2, catalase, almost abolished the dust-mediated enhancement in DNA damage, suggesting a role of O2· and H2O2 in this process. However, the scavengers of singlet oxygen, β-carotene and hydroxyl radical, ethanol and dimethyl sulfoxide had no effect in suppressing the enhancement of DNA damage and lipid peroxidation. These studies suggest that the airborne dust collected soon after the war was contaminated with certain constituent elements which were effective in enhancing lipid peroxidation and causing DNA damage through the generation of O2· and H2O2.

Original languageEnglish
Pages (from-to)205-212
Number of pages8
JournalEnvironment International
Volume24
Issue number1-2
DOIs
Publication statusPublished - Jan 1998

Fingerprint

Membrane Lipids
Dust
lipid
membrane
dust
Lipids
DNA
damage
Singlet Oxygen
Hydroxyl Radical
Deoxyribose
Biological membranes
scavenger
hydroxyl radical
Carotenoids
Dimethyl Sulfoxide
Sugars
Superoxides
Catalase
Hydrogen Peroxide

ASJC Scopus subject areas

  • Environmental Chemistry
  • Environmental Science(all)

Cite this

Airborne dust collected from Kuwait in 1991-1992 augments peroxidation of cellular membrane lipids and enhances DNA damage. / Athar, Mohammad; Iqbal, Mohammad; Beg, Mirza U.; Al-Ajmi, Dhari; Al-Muzaini, Saleh.

In: Environment International, Vol. 24, No. 1-2, 01.1998, p. 205-212.

Research output: Contribution to journalArticle

Athar, Mohammad ; Iqbal, Mohammad ; Beg, Mirza U. ; Al-Ajmi, Dhari ; Al-Muzaini, Saleh. / Airborne dust collected from Kuwait in 1991-1992 augments peroxidation of cellular membrane lipids and enhances DNA damage. In: Environment International. 1998 ; Vol. 24, No. 1-2. pp. 205-212.
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