Acylation stimulating protein (ASP) acute effects on postprandial lipemia and food intake in rodents

J. Saleh; J. E. Blevins; P. J. Havel; J. A. Barrett; D. W. Gietzen; K. Cianflone

doi:10.1038/sj.ijo.0801613

Acylation stimulating protein (ASP) acute effects on postprandial lipemia and food intake in rodents

J. Saleh, J. E. Blevins, P. J. Havel, J. A. Barrett, D. W. Gietzen, K. Cianflone^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

BACKGROUND: In vitro studies have shown that acylation stimulating protein (ASP) stimulates triglyceride (TG) synthesis and storage in adipocytes. We have previously demonstrated that intraperitoneal (i.p.) injection of ASP in C57BL/6J mice accelerated TG clearance following an orally-administered fat load as well as reducing postprandial glucose levels. RESULTS: In the present study, we first examined the effect of i.p. and intracerebroventricular (i.c.v.) injection of ASP on food intake in Sprague - Dawley rats. Intraperitoneal injection resulted in a short-term increase in food intake (maximum increase 29.3% within the first hour, P < 0.025) decreasing thereafter as compared to vehicle alone, i.c.v. Administration of a comparable dose of ASP resulted in a similar but delayed increase in food intake with a maximum at 2 - 4 h, suggesting that the actions of ASP are peripherally mediated. However, there was no significant difference in 24 h food intake with either i.p. or i.c.v. injection. We also examined the effects of ASP on TG clearance in two obese mouse strains with different metabolic profiles: ob/ob (C57BL/6J-Lep^ob) and db/db (C57BLKS/J-Lepr^db). In a crossover design, the response to an oral fat load was determined with and without i.p. injection of exogenous ASP. In ob/ob mice, there was a 44% greater clearance of postprandial TG (area under the curve (AUC) = 245±49 control vs 138±43 mg/dl h with ASP; P< 0.05 by RM ANOVA). The db/db mice showed a greater response, with a 62% decrease in postprandial TG (AUC = 4080 ± 1489 control vs 1540±719 mg/dl h with ASP; P = 0.004 by RM ANOVA). In addition there were decreases in postprandial glucose and non-esterified fatty acid (NEFA) levels in response to ASP. CONCLUSION: These results are the first to report that ASP can increase food intake in rats and also enhance postprandial TG clearance in obese animals. These data therefore support previous in vitro evidence pointing to ASP as a regulator of lipid metabolism.

Original language	English
Pages (from-to)	705-713
Number of pages	9
Journal	International Journal of Obesity
Volume	25
Issue number	5
DOIs	https://doi.org/10.1038/sj.ijo.0801613
Publication status	Published - 2001
Externally published	Yes

Keywords

Acylation stimulating protein
Adipose tissue
Complement C3a
Glucose
Non-esterified fatty acid
Triglyceride

ASJC Scopus subject areas

Medicine (miscellaneous)
Endocrinology, Diabetes and Metabolism
Nutrition and Dietetics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1038/sj.ijo.0801613

Cite this

@article{1894417dafe848679def8cd98ef9f063,

title = "Acylation stimulating protein (ASP) acute effects on postprandial lipemia and food intake in rodents",

abstract = "BACKGROUND: In vitro studies have shown that acylation stimulating protein (ASP) stimulates triglyceride (TG) synthesis and storage in adipocytes. We have previously demonstrated that intraperitoneal (i.p.) injection of ASP in C57BL/6J mice accelerated TG clearance following an orally-administered fat load as well as reducing postprandial glucose levels. RESULTS: In the present study, we first examined the effect of i.p. and intracerebroventricular (i.c.v.) injection of ASP on food intake in Sprague - Dawley rats. Intraperitoneal injection resulted in a short-term increase in food intake (maximum increase 29.3% within the first hour, P < 0.025) decreasing thereafter as compared to vehicle alone, i.c.v. Administration of a comparable dose of ASP resulted in a similar but delayed increase in food intake with a maximum at 2 - 4 h, suggesting that the actions of ASP are peripherally mediated. However, there was no significant difference in 24 h food intake with either i.p. or i.c.v. injection. We also examined the effects of ASP on TG clearance in two obese mouse strains with different metabolic profiles: ob/ob (C57BL/6J-Lepob) and db/db (C57BLKS/J-Leprdb). In a crossover design, the response to an oral fat load was determined with and without i.p. injection of exogenous ASP. In ob/ob mice, there was a 44% greater clearance of postprandial TG (area under the curve (AUC) = 245±49 control vs 138±43 mg/dl h with ASP; P< 0.05 by RM ANOVA). The db/db mice showed a greater response, with a 62% decrease in postprandial TG (AUC = 4080 ± 1489 control vs 1540±719 mg/dl h with ASP; P = 0.004 by RM ANOVA). In addition there were decreases in postprandial glucose and non-esterified fatty acid (NEFA) levels in response to ASP. CONCLUSION: These results are the first to report that ASP can increase food intake in rats and also enhance postprandial TG clearance in obese animals. These data therefore support previous in vitro evidence pointing to ASP as a regulator of lipid metabolism.",

keywords = "Acylation stimulating protein, Adipose tissue, Complement C3a, Glucose, Non-esterified fatty acid, Triglyceride",

author = "J. Saleh and Blevins, {J. E.} and Havel, {P. J.} and Barrett, {J. A.} and Gietzen, {D. W.} and K. Cianflone",

note = "Funding Information: This study was supported by grants from National Science and Engineering Council of Canada (to KC) and National Institutes of Health Grants DK50129, DK07355, DK35747 and NS33347 and the American Diabetes Association research award (PJH and DWG). We would like to acknowledge Ian Murray for expert technical assistance, Steve Ph{\'e}lis for providing the human ASP and quantitation of human ASP in mouse plasma and Magdalena Maslowska for helpful discussions. KC is a chercheur-boursier of the Fonds des Recherches en Sant{\'e} du Qu{\'e}bec.",

year = "2001",

doi = "10.1038/sj.ijo.0801613",

language = "English",

volume = "25",

pages = "705--713",

journal = "International Journal of Obesity",

issn = "0307-0565",

publisher = "Nature Publishing Group",

number = "5",

}

TY - JOUR

T1 - Acylation stimulating protein (ASP) acute effects on postprandial lipemia and food intake in rodents

AU - Saleh, J.

AU - Blevins, J. E.

AU - Havel, P. J.

AU - Barrett, J. A.

AU - Gietzen, D. W.

AU - Cianflone, K.

N1 - Funding Information: This study was supported by grants from National Science and Engineering Council of Canada (to KC) and National Institutes of Health Grants DK50129, DK07355, DK35747 and NS33347 and the American Diabetes Association research award (PJH and DWG). We would like to acknowledge Ian Murray for expert technical assistance, Steve Phélis for providing the human ASP and quantitation of human ASP in mouse plasma and Magdalena Maslowska for helpful discussions. KC is a chercheur-boursier of the Fonds des Recherches en Santé du Québec.

PY - 2001

Y1 - 2001

N2 - BACKGROUND: In vitro studies have shown that acylation stimulating protein (ASP) stimulates triglyceride (TG) synthesis and storage in adipocytes. We have previously demonstrated that intraperitoneal (i.p.) injection of ASP in C57BL/6J mice accelerated TG clearance following an orally-administered fat load as well as reducing postprandial glucose levels. RESULTS: In the present study, we first examined the effect of i.p. and intracerebroventricular (i.c.v.) injection of ASP on food intake in Sprague - Dawley rats. Intraperitoneal injection resulted in a short-term increase in food intake (maximum increase 29.3% within the first hour, P < 0.025) decreasing thereafter as compared to vehicle alone, i.c.v. Administration of a comparable dose of ASP resulted in a similar but delayed increase in food intake with a maximum at 2 - 4 h, suggesting that the actions of ASP are peripherally mediated. However, there was no significant difference in 24 h food intake with either i.p. or i.c.v. injection. We also examined the effects of ASP on TG clearance in two obese mouse strains with different metabolic profiles: ob/ob (C57BL/6J-Lepob) and db/db (C57BLKS/J-Leprdb). In a crossover design, the response to an oral fat load was determined with and without i.p. injection of exogenous ASP. In ob/ob mice, there was a 44% greater clearance of postprandial TG (area under the curve (AUC) = 245±49 control vs 138±43 mg/dl h with ASP; P< 0.05 by RM ANOVA). The db/db mice showed a greater response, with a 62% decrease in postprandial TG (AUC = 4080 ± 1489 control vs 1540±719 mg/dl h with ASP; P = 0.004 by RM ANOVA). In addition there were decreases in postprandial glucose and non-esterified fatty acid (NEFA) levels in response to ASP. CONCLUSION: These results are the first to report that ASP can increase food intake in rats and also enhance postprandial TG clearance in obese animals. These data therefore support previous in vitro evidence pointing to ASP as a regulator of lipid metabolism.

AB - BACKGROUND: In vitro studies have shown that acylation stimulating protein (ASP) stimulates triglyceride (TG) synthesis and storage in adipocytes. We have previously demonstrated that intraperitoneal (i.p.) injection of ASP in C57BL/6J mice accelerated TG clearance following an orally-administered fat load as well as reducing postprandial glucose levels. RESULTS: In the present study, we first examined the effect of i.p. and intracerebroventricular (i.c.v.) injection of ASP on food intake in Sprague - Dawley rats. Intraperitoneal injection resulted in a short-term increase in food intake (maximum increase 29.3% within the first hour, P < 0.025) decreasing thereafter as compared to vehicle alone, i.c.v. Administration of a comparable dose of ASP resulted in a similar but delayed increase in food intake with a maximum at 2 - 4 h, suggesting that the actions of ASP are peripherally mediated. However, there was no significant difference in 24 h food intake with either i.p. or i.c.v. injection. We also examined the effects of ASP on TG clearance in two obese mouse strains with different metabolic profiles: ob/ob (C57BL/6J-Lepob) and db/db (C57BLKS/J-Leprdb). In a crossover design, the response to an oral fat load was determined with and without i.p. injection of exogenous ASP. In ob/ob mice, there was a 44% greater clearance of postprandial TG (area under the curve (AUC) = 245±49 control vs 138±43 mg/dl h with ASP; P< 0.05 by RM ANOVA). The db/db mice showed a greater response, with a 62% decrease in postprandial TG (AUC = 4080 ± 1489 control vs 1540±719 mg/dl h with ASP; P = 0.004 by RM ANOVA). In addition there were decreases in postprandial glucose and non-esterified fatty acid (NEFA) levels in response to ASP. CONCLUSION: These results are the first to report that ASP can increase food intake in rats and also enhance postprandial TG clearance in obese animals. These data therefore support previous in vitro evidence pointing to ASP as a regulator of lipid metabolism.

KW - Acylation stimulating protein

KW - Adipose tissue

KW - Complement C3a

KW - Glucose

KW - Non-esterified fatty acid

KW - Triglyceride

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DO - 10.1038/sj.ijo.0801613

M3 - Article

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AN - SCOPUS:0035011251

SN - 0307-0565

VL - 25

SP - 705

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JO - International Journal of Obesity

JF - International Journal of Obesity

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ER -

Acylation stimulating protein (ASP) acute effects on postprandial lipemia and food intake in rodents

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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