A spectrofluorimetric sequential injection method for the determination of penicillamine using fluorescamine in the presence of β-cyclodextrins

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Abstract

A simple, robust and sensitive sequential injection spectrofluorimetric method for the determination of penicillamine (PA) in pharmaceutical formulations is developed. The method is based on the formation of a highly fluorescent derivative when penicillamine is reacted with fluorescamine (FL) in borate buffer of pH 9.3. The derivative produced is monitored at an emission wavelength of 495 nm using an excitation wavelength of 355 nm. The optimum conditions for the determination of PA with FL were: 3 mM FL, pH 9.3, 5 mM methyl-β-cyclodextrin, sample volume of 75 μL and reagent volume of 75 μL. Furthermore, the effect of various media on the fluorescence intensity of the PA-FL derivative was studied and methyl-β-cyclodextrin was found to give the largest enhancement. A linear dynamic range for the determination of PA of 5-80 ppm was obtained with a sampling frequency of 50 h -1 and a relative standard deviation of less than 2.5%. The method was applied to the determination of PA in pharmaceutical formulations with reasonable recoveries ranging from 101.0-103.1%, indicating that no interference is observed from concomitants usually present in dosage forms.

Original languageEnglish
Pages (from-to)1131-1138
Number of pages8
JournalJournal of Fluorescence
Volume18
Issue number6
DOIs
Publication statusPublished - Nov 2008

Fingerprint

Fluorescamine
Penicillamine
Cyclodextrins
pharmaceutical
Injections
Drug Compounding
Derivatives
interference
Wavelength
Borates
Dosage Forms
Pharmaceutical Preparations
Buffers
Fluorescence
Sampling
Recovery

Keywords

  • β-cyclodextrin
  • Fluorescamine
  • Fluorimetry
  • Penicillamine
  • Sequential injection

ASJC Scopus subject areas

  • Spectroscopy
  • Biochemistry
  • Clinical Biochemistry

Cite this

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title = "A spectrofluorimetric sequential injection method for the determination of penicillamine using fluorescamine in the presence of β-cyclodextrins",
abstract = "A simple, robust and sensitive sequential injection spectrofluorimetric method for the determination of penicillamine (PA) in pharmaceutical formulations is developed. The method is based on the formation of a highly fluorescent derivative when penicillamine is reacted with fluorescamine (FL) in borate buffer of pH 9.3. The derivative produced is monitored at an emission wavelength of 495 nm using an excitation wavelength of 355 nm. The optimum conditions for the determination of PA with FL were: 3 mM FL, pH 9.3, 5 mM methyl-β-cyclodextrin, sample volume of 75 μL and reagent volume of 75 μL. Furthermore, the effect of various media on the fluorescence intensity of the PA-FL derivative was studied and methyl-β-cyclodextrin was found to give the largest enhancement. A linear dynamic range for the determination of PA of 5-80 ppm was obtained with a sampling frequency of 50 h -1 and a relative standard deviation of less than 2.5{\%}. The method was applied to the determination of PA in pharmaceutical formulations with reasonable recoveries ranging from 101.0-103.1{\%}, indicating that no interference is observed from concomitants usually present in dosage forms.",
keywords = "β-cyclodextrin, Fluorescamine, Fluorimetry, Penicillamine, Sequential injection",
author = "Suliman, {Fakhr Eldin O} and Al-Lawati, {Zahra H.} and Al-Kindy, {Salma M Z}",
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AU - Al-Kindy, Salma M Z

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N2 - A simple, robust and sensitive sequential injection spectrofluorimetric method for the determination of penicillamine (PA) in pharmaceutical formulations is developed. The method is based on the formation of a highly fluorescent derivative when penicillamine is reacted with fluorescamine (FL) in borate buffer of pH 9.3. The derivative produced is monitored at an emission wavelength of 495 nm using an excitation wavelength of 355 nm. The optimum conditions for the determination of PA with FL were: 3 mM FL, pH 9.3, 5 mM methyl-β-cyclodextrin, sample volume of 75 μL and reagent volume of 75 μL. Furthermore, the effect of various media on the fluorescence intensity of the PA-FL derivative was studied and methyl-β-cyclodextrin was found to give the largest enhancement. A linear dynamic range for the determination of PA of 5-80 ppm was obtained with a sampling frequency of 50 h -1 and a relative standard deviation of less than 2.5%. The method was applied to the determination of PA in pharmaceutical formulations with reasonable recoveries ranging from 101.0-103.1%, indicating that no interference is observed from concomitants usually present in dosage forms.

AB - A simple, robust and sensitive sequential injection spectrofluorimetric method for the determination of penicillamine (PA) in pharmaceutical formulations is developed. The method is based on the formation of a highly fluorescent derivative when penicillamine is reacted with fluorescamine (FL) in borate buffer of pH 9.3. The derivative produced is monitored at an emission wavelength of 495 nm using an excitation wavelength of 355 nm. The optimum conditions for the determination of PA with FL were: 3 mM FL, pH 9.3, 5 mM methyl-β-cyclodextrin, sample volume of 75 μL and reagent volume of 75 μL. Furthermore, the effect of various media on the fluorescence intensity of the PA-FL derivative was studied and methyl-β-cyclodextrin was found to give the largest enhancement. A linear dynamic range for the determination of PA of 5-80 ppm was obtained with a sampling frequency of 50 h -1 and a relative standard deviation of less than 2.5%. The method was applied to the determination of PA in pharmaceutical formulations with reasonable recoveries ranging from 101.0-103.1%, indicating that no interference is observed from concomitants usually present in dosage forms.

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