Abstract
A simple, robust and sensitive sequential injection spectrofluorimetric method for the determination of penicillamine (PA) in pharmaceutical formulations is developed. The method is based on the formation of a highly fluorescent derivative when penicillamine is reacted with fluorescamine (FL) in borate buffer of pH 9.3. The derivative produced is monitored at an emission wavelength of 495 nm using an excitation wavelength of 355 nm. The optimum conditions for the determination of PA with FL were: 3 mM FL, pH 9.3, 5 mM methyl-β-cyclodextrin, sample volume of 75 μL and reagent volume of 75 μL. Furthermore, the effect of various media on the fluorescence intensity of the PA-FL derivative was studied and methyl-β-cyclodextrin was found to give the largest enhancement. A linear dynamic range for the determination of PA of 5-80 ppm was obtained with a sampling frequency of 50 h -1 and a relative standard deviation of less than 2.5%. The method was applied to the determination of PA in pharmaceutical formulations with reasonable recoveries ranging from 101.0-103.1%, indicating that no interference is observed from concomitants usually present in dosage forms.
| Original language | English |
|---|---|
| Pages (from-to) | 1131-1138 |
| Number of pages | 8 |
| Journal | Journal of Fluorescence |
| Volume | 18 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Nov 2008 |
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Keywords
- β-cyclodextrin
- Fluorescamine
- Fluorimetry
- Penicillamine
- Sequential injection
ASJC Scopus subject areas
- Spectroscopy
- Biochemistry
- Clinical Biochemistry
Cite this
A spectrofluorimetric sequential injection method for the determination of penicillamine using fluorescamine in the presence of β-cyclodextrins. / Suliman, Fakhr Eldin O; Al-Lawati, Zahra H.; Al-Kindy, Salma M Z.
In: Journal of Fluorescence, Vol. 18, No. 6, 11.2008, p. 1131-1138.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A spectrofluorimetric sequential injection method for the determination of penicillamine using fluorescamine in the presence of β-cyclodextrins
AU - Suliman, Fakhr Eldin O
AU - Al-Lawati, Zahra H.
AU - Al-Kindy, Salma M Z
PY - 2008/11
Y1 - 2008/11
N2 - A simple, robust and sensitive sequential injection spectrofluorimetric method for the determination of penicillamine (PA) in pharmaceutical formulations is developed. The method is based on the formation of a highly fluorescent derivative when penicillamine is reacted with fluorescamine (FL) in borate buffer of pH 9.3. The derivative produced is monitored at an emission wavelength of 495 nm using an excitation wavelength of 355 nm. The optimum conditions for the determination of PA with FL were: 3 mM FL, pH 9.3, 5 mM methyl-β-cyclodextrin, sample volume of 75 μL and reagent volume of 75 μL. Furthermore, the effect of various media on the fluorescence intensity of the PA-FL derivative was studied and methyl-β-cyclodextrin was found to give the largest enhancement. A linear dynamic range for the determination of PA of 5-80 ppm was obtained with a sampling frequency of 50 h -1 and a relative standard deviation of less than 2.5%. The method was applied to the determination of PA in pharmaceutical formulations with reasonable recoveries ranging from 101.0-103.1%, indicating that no interference is observed from concomitants usually present in dosage forms.
AB - A simple, robust and sensitive sequential injection spectrofluorimetric method for the determination of penicillamine (PA) in pharmaceutical formulations is developed. The method is based on the formation of a highly fluorescent derivative when penicillamine is reacted with fluorescamine (FL) in borate buffer of pH 9.3. The derivative produced is monitored at an emission wavelength of 495 nm using an excitation wavelength of 355 nm. The optimum conditions for the determination of PA with FL were: 3 mM FL, pH 9.3, 5 mM methyl-β-cyclodextrin, sample volume of 75 μL and reagent volume of 75 μL. Furthermore, the effect of various media on the fluorescence intensity of the PA-FL derivative was studied and methyl-β-cyclodextrin was found to give the largest enhancement. A linear dynamic range for the determination of PA of 5-80 ppm was obtained with a sampling frequency of 50 h -1 and a relative standard deviation of less than 2.5%. The method was applied to the determination of PA in pharmaceutical formulations with reasonable recoveries ranging from 101.0-103.1%, indicating that no interference is observed from concomitants usually present in dosage forms.
KW - β-cyclodextrin
KW - Fluorescamine
KW - Fluorimetry
KW - Penicillamine
KW - Sequential injection
UR - http://www.scopus.com/inward/record.url?scp=56749152267&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=56749152267&partnerID=8YFLogxK
U2 - 10.1007/s10895-008-0363-9
DO - 10.1007/s10895-008-0363-9
M3 - Article
C2 - 18496741
AN - SCOPUS:56749152267
VL - 18
SP - 1131
EP - 1138
JO - Journal of Fluorescence
JF - Journal of Fluorescence
SN - 1053-0509
IS - 6
ER -