Abstract
This paper presents an active mechanical perforation method developed for embryo biopsy in the preimplantation genetic diagnosis (PGD) procedure. This mechanical approach utilizes a tungsten micro-needle with a sub-micrometer tip to perform embryo perforation. The micro-needle is driven longitudinally with sinusoidal or square wave signals at frequencies close to the micro-needle bending resonances in order to excite its lateral vibration modes due to nonlinear modal coupling. It has been demonstrated that, in particular, the lateral cutting method, is effective and suitable for embryo perforation as well as microinjection and micro-dissection. A micro-needle driven with a square wave signal at frequency (∼850Hz) close to the micro-needle first bending resonance frequency has been shown to generate an accurate cutting dimensions with minimum damage to the mouse embryo zona pellucida. A success rate of 88 % was achieved based on eight perforation tasks.
| Original language | English |
|---|---|
| Title of host publication | 2013 ICME International Conference on Complex Medical Engineering, CME 2013 |
| Pages | 313-318 |
| Number of pages | 6 |
| DOIs | |
| Publication status | Published - 2013 |
| Event | 2013 7th ICME International Conference on Complex Medical Engineering, CME 2013 - Beijing, China Duration: May 25 2013 → May 28 2013 |
Other
| Other | 2013 7th ICME International Conference on Complex Medical Engineering, CME 2013 |
|---|---|
| Country | China |
| City | Beijing |
| Period | 5/25/13 → 5/28/13 |
Fingerprint
Keywords
- embryo biopsy
- Microsurgery
- perforation
- PGD
ASJC Scopus subject areas
- Biomedical Engineering
Cite this
A mechanical perforation procedure for embryo biopsy. / Bahadur, Issam M.; Mills, James K.
2013 ICME International Conference on Complex Medical Engineering, CME 2013. 2013. p. 313-318 6548261.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - A mechanical perforation procedure for embryo biopsy
AU - Bahadur, Issam M.
AU - Mills, James K.
PY - 2013
Y1 - 2013
N2 - This paper presents an active mechanical perforation method developed for embryo biopsy in the preimplantation genetic diagnosis (PGD) procedure. This mechanical approach utilizes a tungsten micro-needle with a sub-micrometer tip to perform embryo perforation. The micro-needle is driven longitudinally with sinusoidal or square wave signals at frequencies close to the micro-needle bending resonances in order to excite its lateral vibration modes due to nonlinear modal coupling. It has been demonstrated that, in particular, the lateral cutting method, is effective and suitable for embryo perforation as well as microinjection and micro-dissection. A micro-needle driven with a square wave signal at frequency (∼850Hz) close to the micro-needle first bending resonance frequency has been shown to generate an accurate cutting dimensions with minimum damage to the mouse embryo zona pellucida. A success rate of 88 % was achieved based on eight perforation tasks.
AB - This paper presents an active mechanical perforation method developed for embryo biopsy in the preimplantation genetic diagnosis (PGD) procedure. This mechanical approach utilizes a tungsten micro-needle with a sub-micrometer tip to perform embryo perforation. The micro-needle is driven longitudinally with sinusoidal or square wave signals at frequencies close to the micro-needle bending resonances in order to excite its lateral vibration modes due to nonlinear modal coupling. It has been demonstrated that, in particular, the lateral cutting method, is effective and suitable for embryo perforation as well as microinjection and micro-dissection. A micro-needle driven with a square wave signal at frequency (∼850Hz) close to the micro-needle first bending resonance frequency has been shown to generate an accurate cutting dimensions with minimum damage to the mouse embryo zona pellucida. A success rate of 88 % was achieved based on eight perforation tasks.
KW - embryo biopsy
KW - Microsurgery
KW - perforation
KW - PGD
UR - http://www.scopus.com/inward/record.url?scp=84881527608&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84881527608&partnerID=8YFLogxK
U2 - 10.1109/ICCME.2013.6548261
DO - 10.1109/ICCME.2013.6548261
M3 - Conference contribution
SN - 9781467329699
SP - 313
EP - 318
BT - 2013 ICME International Conference on Complex Medical Engineering, CME 2013
ER -