Abstract
This paper presents an active mechanical perforation method developed for embryo biopsy in the preimplantation genetic diagnosis (PGD) procedure. This mechanical approach utilizes a tungsten micro-needle with a sub-micrometer tip to perform embryo perforation. The micro-needle is driven longitudinally with sinusoidal or square wave signals at frequencies close to the micro-needle bending resonances in order to excite its lateral vibration modes due to nonlinear modal coupling. It has been demonstrated that, in particular, the lateral cutting method, is effective and suitable for embryo perforation as well as microinjection and micro-dissection. A micro-needle driven with a square wave signal at frequency (∼850Hz) close to the micro-needle first bending resonance frequency has been shown to generate an accurate cutting dimensions with minimum damage to the mouse embryo zona pellucida. A success rate of 88 % was achieved based on eight perforation tasks.
Original language | English |
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Title of host publication | 2013 ICME International Conference on Complex Medical Engineering, CME 2013 |
Pages | 313-318 |
Number of pages | 6 |
DOIs | |
Publication status | Published - 2013 |
Event | 2013 7th ICME International Conference on Complex Medical Engineering, CME 2013 - Beijing, China Duration: May 25 2013 → May 28 2013 |
Other
Other | 2013 7th ICME International Conference on Complex Medical Engineering, CME 2013 |
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Country | China |
City | Beijing |
Period | 5/25/13 → 5/28/13 |
Keywords
- embryo biopsy
- Microsurgery
- perforation
- PGD
ASJC Scopus subject areas
- Biomedical Engineering