A mechanical perforation procedure for embryo biopsy

Issam M. Bahadur, James K. Mills

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

This paper presents an active mechanical perforation method developed for embryo biopsy in the preimplantation genetic diagnosis (PGD) procedure. This mechanical approach utilizes a tungsten micro-needle with a sub-micrometer tip to perform embryo perforation. The micro-needle is driven longitudinally with sinusoidal or square wave signals at frequencies close to the micro-needle bending resonances in order to excite its lateral vibration modes due to nonlinear modal coupling. It has been demonstrated that, in particular, the lateral cutting method, is effective and suitable for embryo perforation as well as microinjection and micro-dissection. A micro-needle driven with a square wave signal at frequency (∼850Hz) close to the micro-needle first bending resonance frequency has been shown to generate an accurate cutting dimensions with minimum damage to the mouse embryo zona pellucida. A success rate of 88 % was achieved based on eight perforation tasks.

Original languageEnglish
Title of host publication2013 ICME International Conference on Complex Medical Engineering, CME 2013
Pages313-318
Number of pages6
DOIs
Publication statusPublished - 2013
Event2013 7th ICME International Conference on Complex Medical Engineering, CME 2013 - Beijing, China
Duration: May 25 2013May 28 2013

Other

Other2013 7th ICME International Conference on Complex Medical Engineering, CME 2013
CountryChina
CityBeijing
Period5/25/135/28/13

    Fingerprint

Keywords

  • embryo biopsy
  • Microsurgery
  • perforation
  • PGD

ASJC Scopus subject areas

  • Biomedical Engineering

Cite this

Bahadur, I. M., & Mills, J. K. (2013). A mechanical perforation procedure for embryo biopsy. In 2013 ICME International Conference on Complex Medical Engineering, CME 2013 (pp. 313-318). [6548261] https://doi.org/10.1109/ICCME.2013.6548261