Abstract
A highly sensitive, rapid and economical method for the determination of amlodipine (AM) in biological fluids was developed using a peroxyoxalate chemiluminescence (CL) system in a lab-on-a-chip device. Peroxyoxalate-CL is an indirect type of CL that allows the detection of native fluorophores or compounds derivatized with fluorescent labels. Here, fluorescamine was reacted with AM, and the derivatization product was used in a bis-(2,4,6-trichlorophenyl)oxalate-CL system. Fluorescamine reacts selectively with aliphatic primary amine at neutral or basic pH. As most of the calcium channel blocker and many cardiovascular drugs do not contain primary amine, the developed method is highly selective. The parameters that influenced the CL signal intensity were studied carefully. These included the chip geometry, pH, concentration of reagents used and flow rates. Moreover, we confirmed our previous observation about the effects of imidazole, which is commonly used in the bis-(2,4,6-trichlorophenyl)oxalate-CL system as a catalyst, and found that the signal was significantly improved when imidazole was absent. Under optimized conditions, a calibration curve was obtained with a linear range (10-100 μg/L). The limit of detection was 3μg/L, while the limit of quantification was 10μg/L. Finally the method was applied for the determination of AM in biological fluids successfully.
| Original language | English |
|---|---|
| Pages (from-to) | 1148-1153 |
| Number of pages | 6 |
| Journal | Luminescence |
| Volume | 29 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 2014 |
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Keywords
- Amlodipine
- Chemiluminescence
- Fluorescamine
- Lab on a chip
- Peroxyoxalate
ASJC Scopus subject areas
- Biophysics
- Chemistry (miscellaneous)
Cite this
A lab-on-a-chip device for analysis of amlodipine in biological fluids using peroxyoxalate chemiluminescence system. / Al Lawati, Haider A J; Al-Nadabi, Mira M.; Varma, Gouri B.; Suliman, Fakhr Eldin O; Al-Abri, Hasnaa.
In: Luminescence, Vol. 29, No. 8, 2014, p. 1148-1153.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A lab-on-a-chip device for analysis of amlodipine in biological fluids using peroxyoxalate chemiluminescence system
AU - Al Lawati, Haider A J
AU - Al-Nadabi, Mira M.
AU - Varma, Gouri B.
AU - Suliman, Fakhr Eldin O
AU - Al-Abri, Hasnaa
PY - 2014
Y1 - 2014
N2 - A highly sensitive, rapid and economical method for the determination of amlodipine (AM) in biological fluids was developed using a peroxyoxalate chemiluminescence (CL) system in a lab-on-a-chip device. Peroxyoxalate-CL is an indirect type of CL that allows the detection of native fluorophores or compounds derivatized with fluorescent labels. Here, fluorescamine was reacted with AM, and the derivatization product was used in a bis-(2,4,6-trichlorophenyl)oxalate-CL system. Fluorescamine reacts selectively with aliphatic primary amine at neutral or basic pH. As most of the calcium channel blocker and many cardiovascular drugs do not contain primary amine, the developed method is highly selective. The parameters that influenced the CL signal intensity were studied carefully. These included the chip geometry, pH, concentration of reagents used and flow rates. Moreover, we confirmed our previous observation about the effects of imidazole, which is commonly used in the bis-(2,4,6-trichlorophenyl)oxalate-CL system as a catalyst, and found that the signal was significantly improved when imidazole was absent. Under optimized conditions, a calibration curve was obtained with a linear range (10-100 μg/L). The limit of detection was 3μg/L, while the limit of quantification was 10μg/L. Finally the method was applied for the determination of AM in biological fluids successfully.
AB - A highly sensitive, rapid and economical method for the determination of amlodipine (AM) in biological fluids was developed using a peroxyoxalate chemiluminescence (CL) system in a lab-on-a-chip device. Peroxyoxalate-CL is an indirect type of CL that allows the detection of native fluorophores or compounds derivatized with fluorescent labels. Here, fluorescamine was reacted with AM, and the derivatization product was used in a bis-(2,4,6-trichlorophenyl)oxalate-CL system. Fluorescamine reacts selectively with aliphatic primary amine at neutral or basic pH. As most of the calcium channel blocker and many cardiovascular drugs do not contain primary amine, the developed method is highly selective. The parameters that influenced the CL signal intensity were studied carefully. These included the chip geometry, pH, concentration of reagents used and flow rates. Moreover, we confirmed our previous observation about the effects of imidazole, which is commonly used in the bis-(2,4,6-trichlorophenyl)oxalate-CL system as a catalyst, and found that the signal was significantly improved when imidazole was absent. Under optimized conditions, a calibration curve was obtained with a linear range (10-100 μg/L). The limit of detection was 3μg/L, while the limit of quantification was 10μg/L. Finally the method was applied for the determination of AM in biological fluids successfully.
KW - Amlodipine
KW - Chemiluminescence
KW - Fluorescamine
KW - Lab on a chip
KW - Peroxyoxalate
UR - http://www.scopus.com/inward/record.url?scp=84918574988&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84918574988&partnerID=8YFLogxK
U2 - 10.1002/bio.2675
DO - 10.1002/bio.2675
M3 - Article
C2 - 24782430
AN - SCOPUS:84918574988
VL - 29
SP - 1148
EP - 1153
JO - Luminescence
JF - Luminescence
SN - 1522-7235
IS - 8
ER -