The Significance of PCR Primer Design in Genetic Diversity Studies: Exemplified by Recent Research into the Genetic Structure of Marine Species

Madjid Delghandi*, Marit Pedersen Delghandi, Stephen Goddard

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نتاج البحث

1 اقتباس (Scopus)

ملخص

Genetic markers are widely applied in the study of genetic diversity for many species. The approach incorporates a Polymerase Chain Reaction (PCR) amplification of targeted sequences in the genome. Crucial for the overall success of a PCR experiment is the careful design of synthetic oligonucleotide primers. Ideally designed primer pairs will ensure the efficiency and specificity of the amplification reaction, resulting in a high yield of the desired amplicon. Important criteria such as primer-sequence, −length, and -melting temperature (Tm) are fundamental for the selection of primers and amplification of targeted nucleotide sequences from a DNA template. There are many computational tools available to assist with critical bioinformatics issues related to primer design. These resources allow the user to define parameters and criteria that need to be taken into account when designing primers. Following the initial in silico selection, a primer pair should be further tested in vivo for their amplification efficiency and robustness. Using examples taken from genetic diversity studies in a marine crustacean, this chapter provides outlines for the application of PCR technology and discusses details for the design of primers for the development and characterization of microsatellite and SNP-markers.

اللغة الأصليةEnglish
عنوان منشور المضيفMethods in Molecular Biology
ناشرHumana Press Inc.
الصفحات3-15
عدد الصفحات13
المعرِّفات الرقمية للأشياء
حالة النشرPublished - 2022

سلسلة المنشورات

الاسمMethods in Molecular Biology
مستوى الصوت2392
رقم المعيار الدولي للدوريات (المطبوع)1064-3745
رقم المعيار الدولي للدوريات (الإلكتروني)1940-6029

ASJC Scopus subject areas

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