TY - JOUR
T1 - Single-cell transcriptome identifies FCGR3B upregulated subtype of alveolar macrophages in patients with critical COVID-19
AU - Nassir, Nasna
AU - Tambi, Richa
AU - Bankapur, Asma
AU - Al Heialy, Saba
AU - Karuvantevida, Noushad
AU - Khansaheb, Hamda Hassan
AU - Zehra, Binte
AU - Begum, Ghausia
AU - Hameid, Reem Abdel
AU - Ahmed, Awab
AU - Deesi, Zulfa
AU - Alkhajeh, Abdulmajeed
AU - Uddin, K. M.Furkan
AU - Akter, Hosneara
AU - Safizadeh Shabestari, Seyed Ali
AU - Almidani, Omar
AU - Islam, Amirul
AU - Gaudet, Mellissa
AU - Kandasamy, Richard Kumaran
AU - Loney, Tom
AU - Tayoun, Ahmad Abou
AU - Nowotny, Norbert
AU - Woodbury-Smith, Marc
AU - Rahman, Proton
AU - Kuebler, Wolfgang M.
AU - Yaseen Hachim, Mahmood
AU - Casanova, Jean Laurent
AU - Berdiev, Bakhrom K.
AU - Alsheikh-Ali, Alawi
AU - Uddin, Mohammed
N1 - Publisher Copyright:
© 2021 The Authors
PY - 2021/9/24
Y1 - 2021/9/24
N2 - Understanding host cell heterogeneity is critical for unraveling disease mechanism. Utilizing large-scale single-cell transcriptomics, we analyzed multiple tissue specimens from patients with life-threatening COVID-19 pneumonia, compared with healthy controls. We identified a subtype of monocyte-derived alveolar macrophages (MoAMs) where genes associated with severe COVID-19 comorbidities are significantly upregulated in bronchoalveolar lavage fluid of critical cases. FCGR3B consistently demarcated MoAM subset in different samples from severe COVID-19 cohorts and in CCL3L1-upregulated cells from nasopharyngeal swabs. In silico findings were validated by upregulation of FCGR3B in nasopharyngeal swabs of severe ICU COVID-19 cases, particularly in older patients and those with comorbidities. Additional lines of evidence from transcriptomic data and in vivo of severe COVID-19 cases suggest that FCGR3B may identify a specific subtype of MoAM in patients with severe COVID-19 that may present a novel biomarker for screening and prognosis, as well as a potential therapeutic target.
AB - Understanding host cell heterogeneity is critical for unraveling disease mechanism. Utilizing large-scale single-cell transcriptomics, we analyzed multiple tissue specimens from patients with life-threatening COVID-19 pneumonia, compared with healthy controls. We identified a subtype of monocyte-derived alveolar macrophages (MoAMs) where genes associated with severe COVID-19 comorbidities are significantly upregulated in bronchoalveolar lavage fluid of critical cases. FCGR3B consistently demarcated MoAM subset in different samples from severe COVID-19 cohorts and in CCL3L1-upregulated cells from nasopharyngeal swabs. In silico findings were validated by upregulation of FCGR3B in nasopharyngeal swabs of severe ICU COVID-19 cases, particularly in older patients and those with comorbidities. Additional lines of evidence from transcriptomic data and in vivo of severe COVID-19 cases suggest that FCGR3B may identify a specific subtype of MoAM in patients with severe COVID-19 that may present a novel biomarker for screening and prognosis, as well as a potential therapeutic target.
KW - molecular biology
KW - transcriptomics
KW - virology
UR - http://www.scopus.com/inward/record.url?scp=85122825334&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85122825334&partnerID=8YFLogxK
U2 - 10.1016/j.isci.2021.103030
DO - 10.1016/j.isci.2021.103030
M3 - Article
C2 - 34458692
AN - SCOPUS:85122825334
SN - 2589-0042
VL - 24
JO - iScience
JF - iScience
IS - 9
M1 - 103030
ER -