TY - JOUR
T1 - Mangiferin antagonizes rotenone
T2 - Induced apoptosis through attenuating mitochondrial dysfunction and oxidative stress in SK-N-SH neuroblastoma cells
AU - Kavitha, Mani
AU - Manivasagam, Thamilarasan
AU - Essa, Musthafa Mohamed
AU - Tamilselvam, Kuppusamy
AU - Selvakumar, Govindasamy Pushpavathy
AU - Karthikeyan, Subran
AU - Thenmozhi, Justin Arokiasamy
AU - Subash, Selvaraju
PY - 2014/4
Y1 - 2014/4
N2 - In the present study, using a human neuroblastoma SK-N-SH cells, we explored antioxidant, mitochondrial protective and antiapoptotic properties of mangiferin against rotenone-mediated cytotoxicity. SK-N-SH cells are divided into four experimental groups based on 3-(4,5-dimethyl2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay - untreated cells, cells incubated with rotenone (100 nM), cells treated with mangiferin (20 μg) (pretreatment 4 h before) + rotenone (100 nM) and mangiferin alone treated. 24 h after treatment with rotenone and 28 h after treatment with mangiferin, levels of ATP thiobarbituricacid reactive substances and reduced glutathione and activities of enzymatic antioxidants including superoxide dismutase, catalase and glutathione peroxidise were measured. Finally mitochondrial transmembrane potential and expressions of apoptotic protein were also analysed. Pre-treatment with mangiferin significantly enhanced cell viability, ameliorated decrease in mitochondrial membrane potential and decreased rotenone-induced apoptosis in the cellular model of Parkinson's disease. Moreover oxidative imbalance induced by rotenone was partially rectified by mangiferin. Our results indicated that anti-apoptotic properties of this natural compound due to its antioxidant and mitochondrial protective function protect rotenone induced cytotoxicity.
AB - In the present study, using a human neuroblastoma SK-N-SH cells, we explored antioxidant, mitochondrial protective and antiapoptotic properties of mangiferin against rotenone-mediated cytotoxicity. SK-N-SH cells are divided into four experimental groups based on 3-(4,5-dimethyl2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay - untreated cells, cells incubated with rotenone (100 nM), cells treated with mangiferin (20 μg) (pretreatment 4 h before) + rotenone (100 nM) and mangiferin alone treated. 24 h after treatment with rotenone and 28 h after treatment with mangiferin, levels of ATP thiobarbituricacid reactive substances and reduced glutathione and activities of enzymatic antioxidants including superoxide dismutase, catalase and glutathione peroxidise were measured. Finally mitochondrial transmembrane potential and expressions of apoptotic protein were also analysed. Pre-treatment with mangiferin significantly enhanced cell viability, ameliorated decrease in mitochondrial membrane potential and decreased rotenone-induced apoptosis in the cellular model of Parkinson's disease. Moreover oxidative imbalance induced by rotenone was partially rectified by mangiferin. Our results indicated that anti-apoptotic properties of this natural compound due to its antioxidant and mitochondrial protective function protect rotenone induced cytotoxicity.
KW - Apoptosis
KW - Mangiferin
KW - Mitochondrial dysfunction
KW - Oxidative stress
KW - Rotenone
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UR - http://www.scopus.com/inward/citedby.url?scp=84898869652&partnerID=8YFLogxK
U2 - 10.1007/s11064-014-1249-7
DO - 10.1007/s11064-014-1249-7
M3 - Article
C2 - 24493626
AN - SCOPUS:84898869652
SN - 0364-3190
VL - 39
SP - 668
EP - 676
JO - Neurochemical Research
JF - Neurochemical Research
IS - 4
ER -