TY - JOUR
T1 - In vitro antifungal activity of leaf extract of myrtle (Myrtus Communis L.) against phytopathogenic fungi
AU - Al-Abdali, Mohammed Ali Muhanna
AU - Al-Mahmooli, Issa Hashil
AU - Al-Sadi, Abdullah Mohammed
AU - Al-Sabahi, Jamal Nasser
AU - Al-Ruqaishi, Houda Khlafan
AU - Velazhahan, Rethinasamy
N1 - Publisher Copyright:
© 2019 MIR Labs.
PY - 2019/10
Y1 - 2019/10
N2 - The antifungal properties of leaf extract of the medicinal plant, myrtle (Myrtus communis L.) against Pythium aphanidermatum and Alternaria alternata were determined. The methanolic leaf extract of M. communis inhibited the mycelial growth of P. aphanidermatum in a dose-dependent manner as assessed by poisoned food technique. The leaf extract of myrtle also had inhibitory (17%) effect against conidial germination of A. alternata. The antifungal compound(s) in the methanol extract of M. communis was partially purified by Thin-layer chromatography (TLC) and identified by Gas chromatographymass spectrometry (GC-MS). TLC analysis of methanolic extract of M. communis revealed the presence of a prominent fluorescent spot under UV light (λ = 254 nm) having Rf value of 0.84. The compounds eluted from the TLC plate at Rf 0.84 exhibited antimicrobial activity against A. alternata. GC-MS analysis of the fraction eluted at Rf 0.84 revealed the presence of dodecane and ethyl fluoroacetate. Further studies are required to determine the role of these compounds in antifungal activity of M. communis and the mechanism of action.
AB - The antifungal properties of leaf extract of the medicinal plant, myrtle (Myrtus communis L.) against Pythium aphanidermatum and Alternaria alternata were determined. The methanolic leaf extract of M. communis inhibited the mycelial growth of P. aphanidermatum in a dose-dependent manner as assessed by poisoned food technique. The leaf extract of myrtle also had inhibitory (17%) effect against conidial germination of A. alternata. The antifungal compound(s) in the methanol extract of M. communis was partially purified by Thin-layer chromatography (TLC) and identified by Gas chromatographymass spectrometry (GC-MS). TLC analysis of methanolic extract of M. communis revealed the presence of a prominent fluorescent spot under UV light (λ = 254 nm) having Rf value of 0.84. The compounds eluted from the TLC plate at Rf 0.84 exhibited antimicrobial activity against A. alternata. GC-MS analysis of the fraction eluted at Rf 0.84 revealed the presence of dodecane and ethyl fluoroacetate. Further studies are required to determine the role of these compounds in antifungal activity of M. communis and the mechanism of action.
KW - Alternaria alternata
KW - Antifungal
KW - Myrtus communis
KW - Plant extract
KW - Pythium aphanidermatum
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U2 - 10.35124/bca.2019.19.2.4663
DO - 10.35124/bca.2019.19.2.4663
M3 - Article
AN - SCOPUS:85083252263
SN - 0972-5075
VL - 19
SP - 4663
EP - 4668
JO - Biochemical and Cellular Archives
JF - Biochemical and Cellular Archives
IS - 2
ER -