Fully investigation of rp-hplc analytical method validation parameters for determination of cefixime traces in the different pharmaceutical dosage forms and urine analysis

Mostafa F. Al-Hakkani*, Gamal A. Gouda, Sedky H.A. Hassan, Osman A. Farghaly, Mahmoud M.A. Mohamed

*المؤلف المقابل لهذا العمل

نتاج البحث: المساهمة في مجلةمراجعة النظراء

11 اقتباسات (Scopus)

ملخص

Cefixime (Cfx) is a member of the third generation of Cephalosporin antibiotics. It used on a wide scale in prescribed antibiotic drugs as anti-infection for Gram-positive and Gram-negative microorganisms. The present study aimed to develop an HPLC method of Cfx analysis enjoyed highly linearity, repeatability, robustness, ruggedness, selectivity, rapidly, and economical to use. The chromatographic system depends on the RP-BDS column (250 mm x 4.6 mm x 5 μm). The mobile phase was prepared by mixing Metha-nol: Phosphate buffer (3:7, v/v) at flow rate 1.0 ml/min with wavelength detection at 254 nm, the temperature at 30° C with injection volume 20 μL. The method revealed that satisfied linearity regression R2 (0.9996) with repeatability (0.94%) with DL and QL; 59.3 ng/ml and 179.8 ng/ml respectively. The method showed successful and satisfying results for Cfx in bulk and pharmaceutical formulations and urine samples at low levels.

اللغة الأصليةEnglish
الصفحات (من إلى)631-645
عدد الصفحات15
دوريةActa Pharmaceutica Sciencia
مستوى الصوت59
رقم الإصدار1
المعرِّفات الرقمية للأشياء
حالة النشرPublished - 2021
منشور خارجيًانعم

ASJC Scopus subject areas

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