TY - JOUR
T1 - Dynamics of gametocytes among Plasmodium falciparum clones in natural infections in an area of highly seasonal transmission
AU - Abdel-Wahab, Amel
AU - Abdel-Muhsin, Abdel Muhsin A.
AU - Ali, Eltayeb
AU - Suleiman, Suad
AU - Ahmed, Salah
AU - Walliker, David
AU - Babiker, Hamza A.
N1 - Funding Information:
Financial support: Medical Research Council (United Kingdom); Wellcome Trust; World Health Organization/Training in Tropical Diseases; Gordon Memorial College Trust Fund (United Kingdom); International Atomic Energy Agency.
PY - 2002/6/15
Y1 - 2002/6/15
N2 - The dynamics of gametocyte production in Plasmodium falciparurn clones were studied in inhabitants of an area of highly seasonal malaria transmission in eastern Sudan. Reverse-transcriptase polymerase chain reaction was used to detect expression of 2 genes that encode gametocyte-specific proteins, pfs25 and pfg377, in parasites sampled from individuals throughout one year. Some patients who acquired infections during the wet season were found to harbor subpatent gametocytemia through the following dry season in the apparent absence of mosquito transmission. Genotyping of parasites in multiclonal infections showed considerable fluctuation of gametocyte production by individual clones. The gametocytes present at the end of the dry season provide the most probable source of the genetically complex cyclical malaria outbreaks following the rainy season in this region.
AB - The dynamics of gametocyte production in Plasmodium falciparurn clones were studied in inhabitants of an area of highly seasonal malaria transmission in eastern Sudan. Reverse-transcriptase polymerase chain reaction was used to detect expression of 2 genes that encode gametocyte-specific proteins, pfs25 and pfg377, in parasites sampled from individuals throughout one year. Some patients who acquired infections during the wet season were found to harbor subpatent gametocytemia through the following dry season in the apparent absence of mosquito transmission. Genotyping of parasites in multiclonal infections showed considerable fluctuation of gametocyte production by individual clones. The gametocytes present at the end of the dry season provide the most probable source of the genetically complex cyclical malaria outbreaks following the rainy season in this region.
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U2 - 10.1086/340638
DO - 10.1086/340638
M3 - Article
C2 - 12085337
AN - SCOPUS:0037097792
SN - 0022-1899
VL - 185
SP - 1838
EP - 1842
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 12
ER -
