TY - JOUR
T1 - Diffusion in gel-enzyme linked immunosorbent assay (DIG-ELISA) to record the immunoglobulin response of calves vaccinated with Salmonella
AU - Johnson, Eugene H.
AU - Smith, Bradford P.
AU - Reina-Guerra, Mario
N1 - Funding Information:
The authors thank Stefan Lange and Hakan Nygren of the University of GSteborg in Sweden for useful suggestions in the development of this test. This work was sponsored in part by the Western Regional Research (W112) Funds and the California Milk Advisory Board.
PY - 1984/12
Y1 - 1984/12
N2 - A diffusion in gel-enzyme linked immunosorbent assay (DIG-ELISA) was used to record immunoglobulin development of colostrum-fed calves vaccinated with aromatic dependent (aro-) Salmonella and challenged with either the homologous or a heterologous serotype. IgG was detected by using a peroxidase conjugated rabbit antibovine IgG, whereas IgM and IgA were measured using a double sandwich technique. Although IgG levels to Salmonella endotoxins increased after exposure to Salmonella, they were found to be high in many calves prior to vaccination. However, IgM antibody levels were consistently low prior to vaccination, and their increase was a more reliable indicator of the vaccination and immune status of the calves. IgA levels were generally low and of less predictive value.
AB - A diffusion in gel-enzyme linked immunosorbent assay (DIG-ELISA) was used to record immunoglobulin development of colostrum-fed calves vaccinated with aromatic dependent (aro-) Salmonella and challenged with either the homologous or a heterologous serotype. IgG was detected by using a peroxidase conjugated rabbit antibovine IgG, whereas IgM and IgA were measured using a double sandwich technique. Although IgG levels to Salmonella endotoxins increased after exposure to Salmonella, they were found to be high in many calves prior to vaccination. However, IgM antibody levels were consistently low prior to vaccination, and their increase was a more reliable indicator of the vaccination and immune status of the calves. IgA levels were generally low and of less predictive value.
UR - http://www.scopus.com/inward/record.url?scp=0021712597&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021712597&partnerID=8YFLogxK
U2 - 10.1016/0378-1135(84)90057-9
DO - 10.1016/0378-1135(84)90057-9
M3 - Article
C2 - 6084889
AN - SCOPUS:0021712597
SN - 0378-1135
VL - 10
SP - 71
EP - 86
JO - Veterinary Microbiology
JF - Veterinary Microbiology
IS - 1
ER -
