TY - JOUR
T1 - A sequential injection method for the determination of Tween-80 in natural water samples using a fluorescence enhancement of the dye Eosin-B
AU - Al-Kindy, Salma M.Z.
AU - Suliman, Fakhr Eldin O.
AU - Salama, Salama B.
AU - Aoudia, Mohamed
AU - Al-Bahry, Seif N.
AU - Al-Bahlany, Hamoud S.
PY - 2003/5/1
Y1 - 2003/5/1
N2 - A simple sequential injection (SI) method is reported for the determination of the non-ionic surfactant polyoxyethylene sorbitan monoleate (Tween-80). The proposed method is based on a fluorescence enhancement of the probe 4′,5′-dibromo-2′,7′-dinitro fluorescein, disodium salt (Eosin B) in the presence of a surfactant. The procedure is optimized using the univariate method of optimization. The optimum operating conditions are as follows: reagent concentration, 40 ppm; pH, 4.5; aspirated volumes of sample and reagent, 120 μL and 100 μL, respectively. With the optimum conditions described, linear calibration curves were obtained from 10 - 200 ppm. The detection limit was 1.7 ppm and the maximum relative standard deviation of the method was 2.7% (n = 5). The fluorescence excitation and emission were fixed at λex = 545 nm and λem = 585 nm. The method was successfully applied to the determination of (TW-80) in natural water samples.
AB - A simple sequential injection (SI) method is reported for the determination of the non-ionic surfactant polyoxyethylene sorbitan monoleate (Tween-80). The proposed method is based on a fluorescence enhancement of the probe 4′,5′-dibromo-2′,7′-dinitro fluorescein, disodium salt (Eosin B) in the presence of a surfactant. The procedure is optimized using the univariate method of optimization. The optimum operating conditions are as follows: reagent concentration, 40 ppm; pH, 4.5; aspirated volumes of sample and reagent, 120 μL and 100 μL, respectively. With the optimum conditions described, linear calibration curves were obtained from 10 - 200 ppm. The detection limit was 1.7 ppm and the maximum relative standard deviation of the method was 2.7% (n = 5). The fluorescence excitation and emission were fixed at λex = 545 nm and λem = 585 nm. The method was successfully applied to the determination of (TW-80) in natural water samples.
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U2 - 10.2116/analsci.19.737
DO - 10.2116/analsci.19.737
M3 - Article
C2 - 12769375
AN - SCOPUS:0038823566
SN - 0910-6340
VL - 19
SP - 737
EP - 742
JO - Analytical Sciences
JF - Analytical Sciences
IS - 5
ER -