Zinc induces disorder-to-order transitions in free and membrane-associated Thellungiella salsuginea dehydrins TsDHN-1 and TsDHN-2: a solution CD and solid-state ATR-FTIR study.

Luna N. Rahman, Vladimir V. Bamm, Janine A M Voyer, Graham S T Smith, Lin Chen, Mahmoud W. Yaish, Barbara A. Moffatt, John R. Dutcher, George Harauz

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Dehydrins are intrinsically unstructured proteins that are expressed in plants experiencing extreme environmental conditions such as drought or low temperature. Although their role is not completely understood, it has been suggested that they stabilize proteins and membrane structures during environmental stress and also sequester metals such as zinc. Here, we investigate two dehydrins (denoted as TsDHN-1 and TsDHN-2) from Thellungiella salsuginea. This plant is a crucifer that thrives in the Canadian sub-Arctic (Yukon Territory) where it grows on saline-rich soils and experiences periods of both extreme cold and drought. We show using circular dichroism and attenuated total reflection-Fourier transform infrared spectroscopy that ordered secondary structure is induced and stabilized in these proteins, both in free and vesicle-bound form, by association with zinc. In membrane-associated form, both proteins have an increased proportion of β-strand conformation induced by the cation, in addition to the amphipathic α-helices formed by their constituent K-segments. These results support the hypothesis that dehydrins stabilize plant plasma and organellar membranes in conditions of stress, and further that zinc may be an important co-factor in stabilization. Whereas dehydrins in the cytosol of a plant cell undergoing dehydration or temperature stress form bulk hydrogels and remain primarily disordered, dehydrins with specific membrane- or protein-associations will have induced ordered secondary structures.

Original languageEnglish
Pages (from-to)1485-1502
Number of pages18
JournalAmino Acids
Volume40
Issue number5
DOIs
Publication statusPublished - May 2011
Externally publishedYes

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Fourier Transform Infrared Spectroscopy
Zinc
Drought
Droughts
Membranes
Membrane Proteins
Yukon Territory
Extreme Cold
Intrinsically Disordered Proteins
Membrane structures
Proteins
Hydrogels
Temperature
Plant Cells
Dichroism
Circular Dichroism
Dehydration
Cytosol
Conformations
Cations

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

Cite this

Zinc induces disorder-to-order transitions in free and membrane-associated Thellungiella salsuginea dehydrins TsDHN-1 and TsDHN-2 : a solution CD and solid-state ATR-FTIR study. / Rahman, Luna N.; Bamm, Vladimir V.; Voyer, Janine A M; Smith, Graham S T; Chen, Lin; Yaish, Mahmoud W.; Moffatt, Barbara A.; Dutcher, John R.; Harauz, George.

In: Amino Acids, Vol. 40, No. 5, 05.2011, p. 1485-1502.

Research output: Contribution to journalArticle

Rahman, Luna N. ; Bamm, Vladimir V. ; Voyer, Janine A M ; Smith, Graham S T ; Chen, Lin ; Yaish, Mahmoud W. ; Moffatt, Barbara A. ; Dutcher, John R. ; Harauz, George. / Zinc induces disorder-to-order transitions in free and membrane-associated Thellungiella salsuginea dehydrins TsDHN-1 and TsDHN-2 : a solution CD and solid-state ATR-FTIR study. In: Amino Acids. 2011 ; Vol. 40, No. 5. pp. 1485-1502.
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