YadA, the multifaceted Yersinia adhesin

Yasmin El Tahir, Mikael Skurnik

Research output: Contribution to journalArticle

171 Citations (Scopus)

Abstract

The adhesion protein YadA is encoded by the YadA gene located in the 70-kb virulence plasmid of Yersinia (pYV) that is common to the pathogenic Yersinia species (Y. pestis, Y. pseudotuberculosis and Y. enterocolitica). YadA is a virulence factor of Y. enterocolitica, however, YadA seems to be dispensable for the virulence of Y. pseudotuberculosis, and in wild-type Y. pestis the YadA gene has a frameshift mutation silencing the gene. Expression of the Y. pseudotuberculosis YadA in Y. pestis reduces its virulence. YadA is a homotrimer of ca. 45-kDa subunits that are anchored to the outer membrane via their C-termini, while their N-termini form a globular head on top of a stalk; the 'lollipop'-shaped YadA structure covers the entire bacterial surface giving it hydrophobic properties. The YadA gene expression is induced at 37°C by the temperature-dependent transcriptional activator LcrF. YadA is a multifaceted protein as revealed by its different biological properties. YadA+ bacteria bind to collagens, laminin, fibronectin, intestinal submucosa, mucus, and to hydrophobic surfaces like polystyrene. YadA+ bacteria autoagglutinate in stationary culture and also specifically agglutinate guinea pig red blood cells. YadA is also a potent serum resistance factor as it inhibits the classical pathway of complement. As invasin, it mediates low rate invasion to tissue culture cells. In a rat model of reactive arthritis YadA and specifically YadA-mediated collagen binding is necessary for Y. enterocolitica to induce the disease. Despite of this wealth of information or perhaps because of it, the in vivo role of YadA during infection remains still largely unresolved.

Original languageEnglish
Pages (from-to)209-218
Number of pages10
JournalInternational Journal of Medical Microbiology
Volume291
Issue number3
Publication statusPublished - 2001

Fingerprint

Yersinia
Virulence
Collagen
Classical Complement Pathway
Bacteria
Reactive Arthritis
Frameshift Mutation
R Factors
Polystyrenes
Gene Silencing
Laminin
Virulence Factors
Mucus
Fibronectins
Genes
Guinea Pigs
Proteins
Plasmids
Cell Culture Techniques
Erythrocytes

Keywords

  • Adhesion
  • Extracellular matrix
  • Serum resistance
  • YadA
  • Yersinia

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

YadA, the multifaceted Yersinia adhesin. / El Tahir, Yasmin; Skurnik, Mikael.

In: International Journal of Medical Microbiology, Vol. 291, No. 3, 2001, p. 209-218.

Research output: Contribution to journalArticle

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