Xanthomonas oryzae pv. oryzae infection triggers accumulation of phenolics, defense-related enzymes and thaumatin-like proteins in rice leaves

R. Velazhahan; J. Jayaraj; Salaheddin Khabbaz; R. Sandoss Kumar; S. Muthukrishnan

doi:10.1080/03235400500222172

Xanthomonas oryzae pv. oryzae infection triggers accumulation of phenolics, defense-related enzymes and thaumatin-like proteins in rice leaves

R. Velazhahan^*, J. Jayaraj, Salaheddin Khabbaz, R. Sandoss Kumar, S. Muthukrishnan

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The effect of Xanthomonas oryzae pv. oryzae infection on induction of phenylalanine ammonia-lyase (PAL), peroxidase (PO), phenolics and thaumatin-like proteins (TLPs) in rice was studied. PAL activity increased significantly one day after inoculation with X. o. pv. oryzae and the maximum enzyme activity was observed two days after inoculation. The phenolic content in rice leaves increased significantly one day after inoculation and the maximum accumulation of phenols was observed two days after inoculation. Significant increase in peroxidase activity was observed in rice leaves one day after inoculation with X. o. pv. oryzae. Isozyme analysis indicated that three peroxidase isozymes (PO-1, PO-2 and PO-3) were induced after inoculation with X. o. pv. oryzae. Immunoblot analysis of protein extracts from control and pathogen inoculated rice plants revealed the induced accumulation of 16 and 24 kDa TLPs in rice leaves in response to X. o. pv. oryzae infection. TLP mRNA accumulation was induced strongly in rice leaves in response to infection by X. o. pv. oryzae .

Original language	English
Pages (from-to)	329-339
Number of pages	11
Journal	Archives of Phytopathology and Plant Protection
Volume	39
Issue number	5
DOIs	https://doi.org/10.1080/03235400500222172
Publication status	Published - Oct 1 2006
Externally published	Yes

Keywords

Bacterial leaf blight
Oryza sativa
Peroxidase
Phenolics
Phenylalanine ammonia-lyase
Thaumatin-like proteins

ASJC Scopus subject areas

Agronomy and Crop Science

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10.1080/03235400500222172

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title = "Xanthomonas oryzae pv. oryzae infection triggers accumulation of phenolics, defense-related enzymes and thaumatin-like proteins in rice leaves",

abstract = "The effect of Xanthomonas oryzae pv. oryzae infection on induction of phenylalanine ammonia-lyase (PAL), peroxidase (PO), phenolics and thaumatin-like proteins (TLPs) in rice was studied. PAL activity increased significantly one day after inoculation with X. o. pv. oryzae and the maximum enzyme activity was observed two days after inoculation. The phenolic content in rice leaves increased significantly one day after inoculation and the maximum accumulation of phenols was observed two days after inoculation. Significant increase in peroxidase activity was observed in rice leaves one day after inoculation with X. o. pv. oryzae. Isozyme analysis indicated that three peroxidase isozymes (PO-1, PO-2 and PO-3) were induced after inoculation with X. o. pv. oryzae. Immunoblot analysis of protein extracts from control and pathogen inoculated rice plants revealed the induced accumulation of 16 and 24 kDa TLPs in rice leaves in response to X. o. pv. oryzae infection. TLP mRNA accumulation was induced strongly in rice leaves in response to infection by X. o. pv. oryzae .",

keywords = "Bacterial leaf blight, Oryza sativa, Peroxidase, Phenolics, Phenylalanine ammonia-lyase, Thaumatin-like proteins",

author = "R. Velazhahan and J. Jayaraj and Salaheddin Khabbaz and Kumar, {R. Sandoss} and S. Muthukrishnan",

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T1 - Xanthomonas oryzae pv. oryzae infection triggers accumulation of phenolics, defense-related enzymes and thaumatin-like proteins in rice leaves

AU - Velazhahan, R.

AU - Jayaraj, J.

AU - Khabbaz, Salaheddin

AU - Kumar, R. Sandoss

AU - Muthukrishnan, S.

PY - 2006/10/1

Y1 - 2006/10/1

N2 - The effect of Xanthomonas oryzae pv. oryzae infection on induction of phenylalanine ammonia-lyase (PAL), peroxidase (PO), phenolics and thaumatin-like proteins (TLPs) in rice was studied. PAL activity increased significantly one day after inoculation with X. o. pv. oryzae and the maximum enzyme activity was observed two days after inoculation. The phenolic content in rice leaves increased significantly one day after inoculation and the maximum accumulation of phenols was observed two days after inoculation. Significant increase in peroxidase activity was observed in rice leaves one day after inoculation with X. o. pv. oryzae. Isozyme analysis indicated that three peroxidase isozymes (PO-1, PO-2 and PO-3) were induced after inoculation with X. o. pv. oryzae. Immunoblot analysis of protein extracts from control and pathogen inoculated rice plants revealed the induced accumulation of 16 and 24 kDa TLPs in rice leaves in response to X. o. pv. oryzae infection. TLP mRNA accumulation was induced strongly in rice leaves in response to infection by X. o. pv. oryzae .

AB - The effect of Xanthomonas oryzae pv. oryzae infection on induction of phenylalanine ammonia-lyase (PAL), peroxidase (PO), phenolics and thaumatin-like proteins (TLPs) in rice was studied. PAL activity increased significantly one day after inoculation with X. o. pv. oryzae and the maximum enzyme activity was observed two days after inoculation. The phenolic content in rice leaves increased significantly one day after inoculation and the maximum accumulation of phenols was observed two days after inoculation. Significant increase in peroxidase activity was observed in rice leaves one day after inoculation with X. o. pv. oryzae. Isozyme analysis indicated that three peroxidase isozymes (PO-1, PO-2 and PO-3) were induced after inoculation with X. o. pv. oryzae. Immunoblot analysis of protein extracts from control and pathogen inoculated rice plants revealed the induced accumulation of 16 and 24 kDa TLPs in rice leaves in response to X. o. pv. oryzae infection. TLP mRNA accumulation was induced strongly in rice leaves in response to infection by X. o. pv. oryzae .

KW - Bacterial leaf blight

KW - Oryza sativa

KW - Peroxidase

KW - Phenolics

KW - Phenylalanine ammonia-lyase

KW - Thaumatin-like proteins

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Xanthomonas oryzae pv. oryzae infection triggers accumulation of phenolics, defense-related enzymes and thaumatin-like proteins in rice leaves

Abstract

Keywords

ASJC Scopus subject areas

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