Xanthomonas oryzae pv. oryzae infection triggers accumulation of phenolics, defense-related enzymes and thaumatin-like proteins in rice leaves

R. Velazhahan, J. Jayaraj, Salaheddin Khabbaz, R. Sandoss Kumar, S. Muthukrishnan

Research output: Contribution to journalArticle

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Abstract

The effect of Xanthomonas oryzae pv. oryzae infection on induction of phenylalanine ammonia-lyase (PAL), peroxidase (PO), phenolics and thaumatin-like proteins (TLPs) in rice was studied. PAL activity increased significantly one day after inoculation with X. o. pv. oryzae and the maximum enzyme activity was observed two days after inoculation. The phenolic content in rice leaves increased significantly one day after inoculation and the maximum accumulation of phenols was observed two days after inoculation. Significant increase in peroxidase activity was observed in rice leaves one day after inoculation with X. o. pv. oryzae. Isozyme analysis indicated that three peroxidase isozymes (PO-1, PO-2 and PO-3) were induced after inoculation with X. o. pv. oryzae. Immunoblot analysis of protein extracts from control and pathogen inoculated rice plants revealed the induced accumulation of 16 and 24 kDa TLPs in rice leaves in response to X. o. pv. oryzae infection. TLP mRNA accumulation was induced strongly in rice leaves in response to infection by X. o. pv. oryzae .

Original languageEnglish
Pages (from-to)329-339
Number of pages11
JournalArchives of Phytopathology and Plant Protection
Volume39
Issue number5
DOIs
Publication statusPublished - Oct 1 2006

Fingerprint

Xanthomonas oryzae pv. oryzae
rice protein
Oryza
peroxidase
enzymes
infection
leaves
rice
phenylalanine ammonia-lyase
isozymes
phenols
proteins
enzyme activity
pathogens
extracts

Keywords

  • Bacterial leaf blight
  • Oryza sativa
  • Peroxidase
  • Phenolics
  • Phenylalanine ammonia-lyase
  • Thaumatin-like proteins

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science

Cite this

Xanthomonas oryzae pv. oryzae infection triggers accumulation of phenolics, defense-related enzymes and thaumatin-like proteins in rice leaves. / Velazhahan, R.; Jayaraj, J.; Khabbaz, Salaheddin; Kumar, R. Sandoss; Muthukrishnan, S.

In: Archives of Phytopathology and Plant Protection, Vol. 39, No. 5, 01.10.2006, p. 329-339.

Research output: Contribution to journalArticle

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