Water-Pipe Smoke Exposure-Induced Circulatory Disturbances in Mice, and the Influence of Betaine Supplementation Thereon

Abderrahim Nemmar, Suhail Al-Salam, Sumaya Beegam, Priya Yuvaraju, Abderrahim Oulhaj, Badreldin H. Ali

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background/Aims: It has been shown, both experimentally and clinically, that water-pipe smoke (WPS) exposure adversely affects the cardiovascular system (CVS) through the generation of oxidative stress and inflammation. Betaine, a naturally occurring compound in common foods, has antioxidant and anti-inflammatory actions. However, its potential to mitigate the adverse effect of WPS on the CVS has never been reported before. This is the subject of this study in mice. Methods: Mice were exposed daily for 30 min to either normal air (control), or to WPS for two consecutive weeks. Betaine was administered daily by gavage at a dose of 10mg/kg, 1h before either air or WPS exposure. Results: Betaine mitigated the in vivo prothrombotic effect of WPS in pial arterioles and venules. Moreover, it reversed the WPS-induced decrease in circulating platelets. Likewise, betaine alleviated platelet aggregation in vitro, and the shortening of activated partial thromboplastin time and prothrombin time induced by WPS. Betaine reduced the increase of plasminogen activator inhibitor-1 and fibrinogen concentrations in plasma induced by WPS. Betaine also diminished the WPS-induced increase of plasma concentrations of interleukin 6 and tumor necrosis factor α, and attenuated the increase of lipid peroxidation and superoxide dismutase. Immunohistochemical analysis of the heart revealed an increase in the expression of inducible nitric oxide synthase and cytochrome C by cardiomyocytes of the WPS-exposed mice. These effects were averted by betaine. Conclusion: Our findings suggest that betaine treatment significantly mitigated WPS-induced hypercoagulability, and inflammation, as well as systemic and cardiac oxidative stress.

Original languageEnglish
Pages (from-to)1098-1112
Number of pages15
JournalCellular Physiology and Biochemistry
DOIs
Publication statusAccepted/In press - Feb 27 2017

Fingerprint

Betaine
Smoke
Water
Cardiovascular System
Oxidative Stress
Air
Inflammation
Thrombophilia
Venules
Partial Thromboplastin Time
Plasminogen Activator Inhibitor 1
Prothrombin Time
Arterioles
Nitric Oxide Synthase Type II
Cytochromes
Platelet Aggregation
Cardiac Myocytes
Fibrinogen
Lipid Peroxidation
Superoxide Dismutase

Keywords

  • Betaine
  • Heart
  • Inflammation
  • Nose-only exposure
  • Oxidative stress
  • Thrombosis
  • Water-pipe smoke

ASJC Scopus subject areas

  • Physiology

Cite this

Water-Pipe Smoke Exposure-Induced Circulatory Disturbances in Mice, and the Influence of Betaine Supplementation Thereon. / Nemmar, Abderrahim; Al-Salam, Suhail; Beegam, Sumaya; Yuvaraju, Priya; Oulhaj, Abderrahim; Ali, Badreldin H.

In: Cellular Physiology and Biochemistry, 27.02.2017, p. 1098-1112.

Research output: Contribution to journalArticle

Nemmar, Abderrahim ; Al-Salam, Suhail ; Beegam, Sumaya ; Yuvaraju, Priya ; Oulhaj, Abderrahim ; Ali, Badreldin H. / Water-Pipe Smoke Exposure-Induced Circulatory Disturbances in Mice, and the Influence of Betaine Supplementation Thereon. In: Cellular Physiology and Biochemistry. 2017 ; pp. 1098-1112.
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AU - Ali, Badreldin H.

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