Voltage-gated K+ channels in rat small cerebral arteries

Molecular identity of the functional channels

Sulayma Albarwani, Leah T. Nemetz, Jane A. Madden, Ann A. Tobin, Sarah K. England, Phillip F. Pratt, Nancy J. Rusch

Research output: Contribution to journalArticle

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Abstract

Voltage-gated potassium (Kv) channels represent an important dilator influence in the cerebral circulation, but the composition of these tetrameric ion channels remains unclear. The goals of the present study were to evaluate the contribution of Kv1 family channels to the resting membrane potential and diameter of small rat cerebral arteries, and to identify the α-subunit composition of these channels using patch-clamp, molecular and immunological techniques. Initial studies indicated that 1 μmol l-1 correolide (COR), a specific antagonist of Kv1 channels, depolarized vascular smooth muscle cells (VSMCs) in pressurized (60 mmHg cerebral arteries from -55 ± 1 mV to -34 ± 1 mV, and reduced the resting diameter from 152 ± 15 μm to 103 ± 20 μm. In patch clamped VSMCs from these arteries, COR-sensitive Kv1 current accounted for 65% of total outward Kv current and was observed at physiological membrane potentials. RT-PCR identified mRNA encoding each of the six classical Kv1 α-subunits, Kv1.1-1.6, in rat cerebral arteries. However, only the Kv1.2 and 1.5 proteins were detected by Western blot. The expression of these proteins in VSMCs was confirmed by immunocytochemistry and co-immunoprecipitation of Kv1.2 and 1.5 from VSMC membranes suggested Kv1.2/1.5 channel assembly. Subsequently, the pharmacological and voltage-sensitive properties of Kv1 current in VSMCs were found to be consistent with a predominant expression of Kv1.2/1.5 heterotetrameric channels. The findings of this study suggest that Kv1.2/1.5 heterotetramers are preferentially expressed in rat cerebral VSMCs, and that these channels contribute to the resting membrane potential and diameter of rat small cerebral arteries.

Original languageEnglish
Pages (from-to)751-763
Number of pages13
JournalJournal of Physiology
Volume551
Issue number3
DOIs
Publication statusPublished - Sep 15 2003

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Voltage-Gated Potassium Channels
Cerebral Arteries
Vascular Smooth Muscle
Smooth Muscle Myocytes
Membrane Potentials
Kv1.2 Potassium Channel
Cerebrovascular Circulation
Immunologic Techniques
Patch-Clamp Techniques
Ion Channels
Immunoprecipitation
Proteins
Arteries
Western Blotting
Immunohistochemistry
Cell Membrane
Pharmacology
Polymerase Chain Reaction
Messenger RNA

ASJC Scopus subject areas

  • Physiology

Cite this

Voltage-gated K+ channels in rat small cerebral arteries : Molecular identity of the functional channels. / Albarwani, Sulayma; Nemetz, Leah T.; Madden, Jane A.; Tobin, Ann A.; England, Sarah K.; Pratt, Phillip F.; Rusch, Nancy J.

In: Journal of Physiology, Vol. 551, No. 3, 15.09.2003, p. 751-763.

Research output: Contribution to journalArticle

Albarwani, Sulayma ; Nemetz, Leah T. ; Madden, Jane A. ; Tobin, Ann A. ; England, Sarah K. ; Pratt, Phillip F. ; Rusch, Nancy J. / Voltage-gated K+ channels in rat small cerebral arteries : Molecular identity of the functional channels. In: Journal of Physiology. 2003 ; Vol. 551, No. 3. pp. 751-763.
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