Visualization of gene expression of short and long forms of prolactin receptor in rat reproductive tissues

A. Ouhtit, G. Morel, P. A. Kelly

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Prolactin receptor gene expression was visualized in various tissues by in situ hybridization. Probes specific to the intracellular domains of the short and long form of receptor were prepared. The specificity of these signals was controlled by competition with excess unlabeled homologous probes or heterologous probes; moreover, some tissues, such as penis and vagina, show no expression of either form of receptor mRNA. Macroautoradiogram signals (optical density) were quantified and expressed in arbitrary units. The long form of receptor mRNA was preferentially expressed in testis, epididymis, prostate, seminal vesicle, and mammary gland from lactating animals, whereas the expression of the two forms of mRNA was equivalent in ovary, uterus, and pregnant mammary gland. Signals were also localized at the light microscopic level to individual cells. This approach has permitted the precise localization of prolactin receptor mRNAs in reproductive tissues. Actions of prolactin have not been demonstrated in all tissues expressing receptor transcripts; thus it will be interesting to correlate the expression of long and short forms of receptor with specific functions.

Original languageEnglish
Pages (from-to)528-536
Number of pages9
JournalBiology of Reproduction
Volume49
Issue number3
Publication statusPublished - 1993

Fingerprint

Prolactin Receptors
Gene Expression
Messenger RNA
Animal Mammary Glands
Seminal Vesicles
Epididymis
Penis
Vagina
Human Mammary Glands
Prolactin
Uterus
In Situ Hybridization
Testis
Prostate
Ovary
Light

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Visualization of gene expression of short and long forms of prolactin receptor in rat reproductive tissues. / Ouhtit, A.; Morel, G.; Kelly, P. A.

In: Biology of Reproduction, Vol. 49, No. 3, 1993, p. 528-536.

Research output: Contribution to journalArticle

@article{62e6b0fda05f4a0c9635e96588ca2dcb,
title = "Visualization of gene expression of short and long forms of prolactin receptor in rat reproductive tissues",
abstract = "Prolactin receptor gene expression was visualized in various tissues by in situ hybridization. Probes specific to the intracellular domains of the short and long form of receptor were prepared. The specificity of these signals was controlled by competition with excess unlabeled homologous probes or heterologous probes; moreover, some tissues, such as penis and vagina, show no expression of either form of receptor mRNA. Macroautoradiogram signals (optical density) were quantified and expressed in arbitrary units. The long form of receptor mRNA was preferentially expressed in testis, epididymis, prostate, seminal vesicle, and mammary gland from lactating animals, whereas the expression of the two forms of mRNA was equivalent in ovary, uterus, and pregnant mammary gland. Signals were also localized at the light microscopic level to individual cells. This approach has permitted the precise localization of prolactin receptor mRNAs in reproductive tissues. Actions of prolactin have not been demonstrated in all tissues expressing receptor transcripts; thus it will be interesting to correlate the expression of long and short forms of receptor with specific functions.",
author = "A. Ouhtit and G. Morel and Kelly, {P. A.}",
year = "1993",
language = "English",
volume = "49",
pages = "528--536",
journal = "Biology of Reproduction",
issn = "0006-3363",
publisher = "Society for the Study of Reproduction",
number = "3",

}

TY - JOUR

T1 - Visualization of gene expression of short and long forms of prolactin receptor in rat reproductive tissues

AU - Ouhtit, A.

AU - Morel, G.

AU - Kelly, P. A.

PY - 1993

Y1 - 1993

N2 - Prolactin receptor gene expression was visualized in various tissues by in situ hybridization. Probes specific to the intracellular domains of the short and long form of receptor were prepared. The specificity of these signals was controlled by competition with excess unlabeled homologous probes or heterologous probes; moreover, some tissues, such as penis and vagina, show no expression of either form of receptor mRNA. Macroautoradiogram signals (optical density) were quantified and expressed in arbitrary units. The long form of receptor mRNA was preferentially expressed in testis, epididymis, prostate, seminal vesicle, and mammary gland from lactating animals, whereas the expression of the two forms of mRNA was equivalent in ovary, uterus, and pregnant mammary gland. Signals were also localized at the light microscopic level to individual cells. This approach has permitted the precise localization of prolactin receptor mRNAs in reproductive tissues. Actions of prolactin have not been demonstrated in all tissues expressing receptor transcripts; thus it will be interesting to correlate the expression of long and short forms of receptor with specific functions.

AB - Prolactin receptor gene expression was visualized in various tissues by in situ hybridization. Probes specific to the intracellular domains of the short and long form of receptor were prepared. The specificity of these signals was controlled by competition with excess unlabeled homologous probes or heterologous probes; moreover, some tissues, such as penis and vagina, show no expression of either form of receptor mRNA. Macroautoradiogram signals (optical density) were quantified and expressed in arbitrary units. The long form of receptor mRNA was preferentially expressed in testis, epididymis, prostate, seminal vesicle, and mammary gland from lactating animals, whereas the expression of the two forms of mRNA was equivalent in ovary, uterus, and pregnant mammary gland. Signals were also localized at the light microscopic level to individual cells. This approach has permitted the precise localization of prolactin receptor mRNAs in reproductive tissues. Actions of prolactin have not been demonstrated in all tissues expressing receptor transcripts; thus it will be interesting to correlate the expression of long and short forms of receptor with specific functions.

UR - http://www.scopus.com/inward/record.url?scp=0027261163&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027261163&partnerID=8YFLogxK

M3 - Article

VL - 49

SP - 528

EP - 536

JO - Biology of Reproduction

JF - Biology of Reproduction

SN - 0006-3363

IS - 3

ER -