Visualization of gene expression of prolactin receptors (PRL-R) by in situ hybridization, in Typhlonectes compressicaudus, a gymnophionan amphibian

Jean Marie Exbrayat, Allal Ouhtit, Gérard Morel

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The expression of the short and long forms of prolactin receptors (PRL-R) mRNA was studied in various types of tissues from Typhlonectes compressicaudus, an amphibian, by quantitative in situ hybridization. Both forms were expressed in all the types of tissues studied. In the liver, small intestine and hypophysis, the mRNA coding for the short form of PRL-R was more strongly expressed than the mRNA coding for the long form and vice-versa for the stomach, spleen and kidneys. In the female liver, quantification showed a higher value of mRNA expression mid-way through pregnancy than during the sexual inactivity period. This result was found to be correlated with the reserve function of the liver. In the kidney and small intestine, the presence of PRL-R was correlated with the hydromineral function. A comparison with certain mammals was also established. These results confirm the ubiquity of PRL effects on metabolic regulation, and suggest a phylogenic conservation of its receptors.

Original languageEnglish
Pages (from-to)1915-1928
Number of pages14
JournalLife Sciences
Volume61
Issue number19
DOIs
Publication statusPublished - Oct 3 1997

Fingerprint

Prolactin Receptors
Amphibians
Gene expression
In Situ Hybridization
Visualization
Liver
Gene Expression
Messenger RNA
Small Intestine
Tissue
Kidney
Mammals
Pituitary Gland
Conservation
Stomach
Spleen
Pregnancy

Keywords

  • Gene expression
  • Metabolic regulation
  • MRNA
  • Prolactin receptors
  • Typhlonectes compressicaudus

ASJC Scopus subject areas

  • Pharmacology

Cite this

Visualization of gene expression of prolactin receptors (PRL-R) by in situ hybridization, in Typhlonectes compressicaudus, a gymnophionan amphibian. / Exbrayat, Jean Marie; Ouhtit, Allal; Morel, Gérard.

In: Life Sciences, Vol. 61, No. 19, 03.10.1997, p. 1915-1928.

Research output: Contribution to journalArticle

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abstract = "The expression of the short and long forms of prolactin receptors (PRL-R) mRNA was studied in various types of tissues from Typhlonectes compressicaudus, an amphibian, by quantitative in situ hybridization. Both forms were expressed in all the types of tissues studied. In the liver, small intestine and hypophysis, the mRNA coding for the short form of PRL-R was more strongly expressed than the mRNA coding for the long form and vice-versa for the stomach, spleen and kidneys. In the female liver, quantification showed a higher value of mRNA expression mid-way through pregnancy than during the sexual inactivity period. This result was found to be correlated with the reserve function of the liver. In the kidney and small intestine, the presence of PRL-R was correlated with the hydromineral function. A comparison with certain mammals was also established. These results confirm the ubiquity of PRL effects on metabolic regulation, and suggest a phylogenic conservation of its receptors.",
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