Variation in interferon sensitivity and induction between Usutu and West Nile (lineages 1 and 2) viruses

Giulia Cacciotti; Beniamino Caputo; Carla Selvaggi; Andrea la Sala; Laura Vitiello; Diawo Diallo; Cornelia Ceianu; Guido Antonelli; Norbert Nowotny; Carolina Scagnolari

doi:10.1016/j.virol.2015.07.015

Variation in interferon sensitivity and induction between Usutu and West Nile (lineages 1 and 2) viruses

Giulia Cacciotti, Beniamino Caputo, Carla Selvaggi, Andrea la Sala, Laura Vitiello, Diawo Diallo, Cornelia Ceianu, Guido Antonelli, Norbert Nowotny, Carolina Scagnolari^*

^*Corresponding author for this work

Microbiology & Immunology

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

Given the pivotal role of monocyte-derived dendritic cells (DCs) in determining the magnitude of the antiviral innate immune response, we sought to determine whether Usutu virus (USUV) and West Nile virus (WNV) lineages (L)1 and L2 can infect DCs and affect the rate of type I interferon (IFN) activation. The sensitivity of these viruses to types I and III IFNs was also compared. We found that USUV can infect DCs, induce higher antiviral activities, IFN alpha subtypes and the IFN stimulated gene (ISG)15 pathway, and is more sensitive to types I and III IFNs than WNVs. In contrast, we confirmed that IFN alpha/beta subtypes were more effective against WNV L2 than WNV L1. However, the replication kinetics, induction of IFN alpha subtypes and ISGs in DCs and the sensitivity to IFN lambda 1-3 did not differ between WNV L1 and L2.

Original language	English
Pages (from-to)	189-198
Number of pages	10
Journal	Virology
Volume	485
DOIs	https://doi.org/10.1016/j.virol.2015.07.015
Publication status	Published - Nov 1 2015

Keywords

IFN
Interferon
Monocyte-derived dendritic cells
USUV
Usutu virus
WNV
West Nile virus

ASJC Scopus subject areas

Virology

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10.1016/j.virol.2015.07.015

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title = "Variation in interferon sensitivity and induction between Usutu and West Nile (lineages 1 and 2) viruses",

abstract = "Given the pivotal role of monocyte-derived dendritic cells (DCs) in determining the magnitude of the antiviral innate immune response, we sought to determine whether Usutu virus (USUV) and West Nile virus (WNV) lineages (L)1 and L2 can infect DCs and affect the rate of type I interferon (IFN) activation. The sensitivity of these viruses to types I and III IFNs was also compared. We found that USUV can infect DCs, induce higher antiviral activities, IFN alpha subtypes and the IFN stimulated gene (ISG)15 pathway, and is more sensitive to types I and III IFNs than WNVs. In contrast, we confirmed that IFN alpha/beta subtypes were more effective against WNV L2 than WNV L1. However, the replication kinetics, induction of IFN alpha subtypes and ISGs in DCs and the sensitivity to IFN lambda 1-3 did not differ between WNV L1 and L2.",

keywords = "IFN, Interferon, Monocyte-derived dendritic cells, USUV, Usutu virus, WNV, West Nile virus",

author = "Giulia Cacciotti and Beniamino Caputo and Carla Selvaggi and {la Sala}, Andrea and Laura Vitiello and Diawo Diallo and Cornelia Ceianu and Guido Antonelli and Norbert Nowotny and Carolina Scagnolari",

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doi = "10.1016/j.virol.2015.07.015",

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AU - Cacciotti, Giulia

AU - Caputo, Beniamino

AU - Selvaggi, Carla

AU - la Sala, Andrea

AU - Vitiello, Laura

AU - Diallo, Diawo

AU - Ceianu, Cornelia

AU - Antonelli, Guido

AU - Nowotny, Norbert

AU - Scagnolari, Carolina

PY - 2015/11/1

Y1 - 2015/11/1

N2 - Given the pivotal role of monocyte-derived dendritic cells (DCs) in determining the magnitude of the antiviral innate immune response, we sought to determine whether Usutu virus (USUV) and West Nile virus (WNV) lineages (L)1 and L2 can infect DCs and affect the rate of type I interferon (IFN) activation. The sensitivity of these viruses to types I and III IFNs was also compared. We found that USUV can infect DCs, induce higher antiviral activities, IFN alpha subtypes and the IFN stimulated gene (ISG)15 pathway, and is more sensitive to types I and III IFNs than WNVs. In contrast, we confirmed that IFN alpha/beta subtypes were more effective against WNV L2 than WNV L1. However, the replication kinetics, induction of IFN alpha subtypes and ISGs in DCs and the sensitivity to IFN lambda 1-3 did not differ between WNV L1 and L2.

AB - Given the pivotal role of monocyte-derived dendritic cells (DCs) in determining the magnitude of the antiviral innate immune response, we sought to determine whether Usutu virus (USUV) and West Nile virus (WNV) lineages (L)1 and L2 can infect DCs and affect the rate of type I interferon (IFN) activation. The sensitivity of these viruses to types I and III IFNs was also compared. We found that USUV can infect DCs, induce higher antiviral activities, IFN alpha subtypes and the IFN stimulated gene (ISG)15 pathway, and is more sensitive to types I and III IFNs than WNVs. In contrast, we confirmed that IFN alpha/beta subtypes were more effective against WNV L2 than WNV L1. However, the replication kinetics, induction of IFN alpha subtypes and ISGs in DCs and the sensitivity to IFN lambda 1-3 did not differ between WNV L1 and L2.

KW - IFN

KW - Interferon

KW - Monocyte-derived dendritic cells

KW - USUV

KW - Usutu virus

KW - WNV

KW - West Nile virus

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Variation in interferon sensitivity and induction between Usutu and West Nile (lineages 1 and 2) viruses

Abstract

Keywords

ASJC Scopus subject areas

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