Usutu virus in wild birds in northern Italy

G. Manarolla; T. Bakonyi; D. Gallazzi; L. Crosta; H. Weissenböck; G. M. Dorrestein; N. Nowotny

doi:10.1016/j.vetmic.2009.07.036

Usutu virus in wild birds in northern Italy

G. Manarolla^*, T. Bakonyi, D. Gallazzi, L. Crosta, H. Weissenböck, G. M. Dorrestein, N. Nowotny

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

88 Citations (Scopus)

Abstract

Usutu virus (USUV) infection was diagnosed in two free-living blackbirds and in three captive owls belonging to two different species in northern Italy in the summers of 2006-2008. Diagnosis was established by immunohistochemistry and RT-PCR. RT-PCR was performed on frozen and on paraffin-embedded tissues (PET), respectively. From the frozen samples a partial sequence of the putative USUV E and NS1 proteins (1229 bp) was determined, whereas partial sequences of the putative NS3 (278 bp) and NS5 (159 bp) proteins were obtained from PET. Additionally, one partial sequence (163 bp) of the putative 3′UTR region was determined from all samples. Sequencing of the amplification products revealed 99.8-100% nucleotide identity of the Italian USUV strains to those from other central European countries.

Original language	English
Pages (from-to)	159-163
Number of pages	5
Journal	Veterinary Microbiology
Volume	141
Issue number	1-2
DOIs	https://doi.org/10.1016/j.vetmic.2009.07.036
Publication status	Published - Feb 24 2010
Externally published	Yes

Keywords

Immunohistochemistry
RT-PCR
Sequencing
Usutu virus
Wild birds

ASJC Scopus subject areas

Microbiology
General Veterinary

Access to Document

10.1016/j.vetmic.2009.07.036

Cite this

@article{dea896358c4d41e89b313787216bf63e,

title = "Usutu virus in wild birds in northern Italy",

abstract = "Usutu virus (USUV) infection was diagnosed in two free-living blackbirds and in three captive owls belonging to two different species in northern Italy in the summers of 2006-2008. Diagnosis was established by immunohistochemistry and RT-PCR. RT-PCR was performed on frozen and on paraffin-embedded tissues (PET), respectively. From the frozen samples a partial sequence of the putative USUV E and NS1 proteins (1229 bp) was determined, whereas partial sequences of the putative NS3 (278 bp) and NS5 (159 bp) proteins were obtained from PET. Additionally, one partial sequence (163 bp) of the putative 3′UTR region was determined from all samples. Sequencing of the amplification products revealed 99.8-100% nucleotide identity of the Italian USUV strains to those from other central European countries.",

keywords = "Immunohistochemistry, RT-PCR, Sequencing, Usutu virus, Wild birds",

author = "G. Manarolla and T. Bakonyi and D. Gallazzi and L. Crosta and H. Weissenb{\"o}ck and Dorrestein, {G. M.} and N. Nowotny",

year = "2010",

month = feb,

day = "24",

doi = "10.1016/j.vetmic.2009.07.036",

language = "English",

volume = "141",

pages = "159--163",

journal = "Veterinary Microbiology",

issn = "0378-1135",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - Usutu virus in wild birds in northern Italy

AU - Manarolla, G.

AU - Bakonyi, T.

AU - Gallazzi, D.

AU - Crosta, L.

AU - Weissenböck, H.

AU - Dorrestein, G. M.

AU - Nowotny, N.

PY - 2010/2/24

Y1 - 2010/2/24

N2 - Usutu virus (USUV) infection was diagnosed in two free-living blackbirds and in three captive owls belonging to two different species in northern Italy in the summers of 2006-2008. Diagnosis was established by immunohistochemistry and RT-PCR. RT-PCR was performed on frozen and on paraffin-embedded tissues (PET), respectively. From the frozen samples a partial sequence of the putative USUV E and NS1 proteins (1229 bp) was determined, whereas partial sequences of the putative NS3 (278 bp) and NS5 (159 bp) proteins were obtained from PET. Additionally, one partial sequence (163 bp) of the putative 3′UTR region was determined from all samples. Sequencing of the amplification products revealed 99.8-100% nucleotide identity of the Italian USUV strains to those from other central European countries.

AB - Usutu virus (USUV) infection was diagnosed in two free-living blackbirds and in three captive owls belonging to two different species in northern Italy in the summers of 2006-2008. Diagnosis was established by immunohistochemistry and RT-PCR. RT-PCR was performed on frozen and on paraffin-embedded tissues (PET), respectively. From the frozen samples a partial sequence of the putative USUV E and NS1 proteins (1229 bp) was determined, whereas partial sequences of the putative NS3 (278 bp) and NS5 (159 bp) proteins were obtained from PET. Additionally, one partial sequence (163 bp) of the putative 3′UTR region was determined from all samples. Sequencing of the amplification products revealed 99.8-100% nucleotide identity of the Italian USUV strains to those from other central European countries.

KW - Immunohistochemistry

KW - RT-PCR

KW - Sequencing

KW - Usutu virus

KW - Wild birds

UR - http://www.scopus.com/inward/record.url?scp=75149128022&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=75149128022&partnerID=8YFLogxK

U2 - 10.1016/j.vetmic.2009.07.036

DO - 10.1016/j.vetmic.2009.07.036

M3 - Article

C2 - 19720475

AN - SCOPUS:75149128022

SN - 0378-1135

VL - 141

SP - 159

EP - 163

JO - Veterinary Microbiology

JF - Veterinary Microbiology

IS - 1-2

ER -

Usutu virus in wild birds in northern Italy

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this