Usefulness of Kato-Katz and trichrome staining as diagnostic methods for parasitic infections in clinical laboratories

Mohamed A. Idris, Ayda M. Al-Jabri

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Objectives: To assess the efficacy of the Kato-Katz technique and to re-evaluate other routine procedures conducted in the Microbiology Clinical Laboratory at Sultan Qaboos University Hospital (SQUH) and to throw light on the prevalence of intestinal parasitic infections among a small group of food handlers in Muscat. Method: Faecal samples collected from food handlers were examined using five parasitological techniques. Results: Out of 100 faecal samples, 53 were positive for one or more of 11 intestinal parasites. The Kato-Katz and trichrome stain methods were found superior to the other techniques in detecting helminthic and protozoan infections, respectively. The auramine stain was useful only in detecting Cryptosporidiumparvum oocysts. Conclusion: A combination of trichrome stain and Kato-Katz techniques for stool examination is sufficient and recommended for busy laboratories; auramine stain should be applied only to samples with suspected cryptosporidal infections.

Original languageEnglish
Pages (from-to)65-68
Number of pages4
JournalSultan Qaboos University Medical Journal
Volume3
Issue number2
Publication statusPublished - Oct 1 2001

Fingerprint

Parasitic Diseases
Benzophenoneidum
Staining and Labeling
Coloring Agents
Protozoan Infections
Oman
Food
Oocysts
Microbiology
Parasites
Infection
trichrome stain

Keywords

  • Concentration
  • Food handlers
  • Intestinal parasites
  • Kato-katz
  • Oman
  • Trichrome and auramine stains
  • Wet preparation

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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abstract = "Objectives: To assess the efficacy of the Kato-Katz technique and to re-evaluate other routine procedures conducted in the Microbiology Clinical Laboratory at Sultan Qaboos University Hospital (SQUH) and to throw light on the prevalence of intestinal parasitic infections among a small group of food handlers in Muscat. Method: Faecal samples collected from food handlers were examined using five parasitological techniques. Results: Out of 100 faecal samples, 53 were positive for one or more of 11 intestinal parasites. The Kato-Katz and trichrome stain methods were found superior to the other techniques in detecting helminthic and protozoan infections, respectively. The auramine stain was useful only in detecting Cryptosporidiumparvum oocysts. Conclusion: A combination of trichrome stain and Kato-Katz techniques for stool examination is sufficient and recommended for busy laboratories; auramine stain should be applied only to samples with suspected cryptosporidal infections.",
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AU - Idris, Mohamed A.

AU - Al-Jabri, Ayda M.

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N2 - Objectives: To assess the efficacy of the Kato-Katz technique and to re-evaluate other routine procedures conducted in the Microbiology Clinical Laboratory at Sultan Qaboos University Hospital (SQUH) and to throw light on the prevalence of intestinal parasitic infections among a small group of food handlers in Muscat. Method: Faecal samples collected from food handlers were examined using five parasitological techniques. Results: Out of 100 faecal samples, 53 were positive for one or more of 11 intestinal parasites. The Kato-Katz and trichrome stain methods were found superior to the other techniques in detecting helminthic and protozoan infections, respectively. The auramine stain was useful only in detecting Cryptosporidiumparvum oocysts. Conclusion: A combination of trichrome stain and Kato-Katz techniques for stool examination is sufficient and recommended for busy laboratories; auramine stain should be applied only to samples with suspected cryptosporidal infections.

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