Toxin production by melon root rot fungus, Monosporascus cannonballus

Amna Yaqoob Al-Rawahi, Issa Hashil Al-Mahmooli, Abdullah Al-Sadi, Jamal Nasser Al-Sabahi, Velazhahan Rethinasamy

Research output: Contribution to journalArticle

Abstract

A study was conducted on the possible in vitro production of phytotoxins by the melon root rot pathogen, Monosporascus cannonballus. The culture filtrate of a highly virulent isolate of the fungus was tested for its phytotoxic activity on cucumber by detached leaf assay, electrolyte leakage assay and seedling vigour test. In detached leaf bioassay, necrotic symptoms developed on cucumber leaves 5–7 days after application of the culture filtrate. The culture filtrate of M. cannonballus caused substantial reduction in seed germination, vigor of cucumber seedlings and an increase in electrolyte leakage from leaf tissues compared to the control. Studies on the effect of heat and dialysis on the phytotoxic activity of the culture filtrate revealed that the toxic metabolites are thermostable and have low molecular weight (<14 kDa). To purify the toxin, the culture filtrate was extracted with different solvents which were then tested separately for their phytotoxicity. The results revealed that the water fraction after extraction with chloroform retained the highest phytotoxic activity. The toxin was partially purified by thin layer chromatography (TLC) and the fluorescent compound eluted with an Rf value of 0.6 was analyzed by gas chromatography-mass spectrometry (GC-MS) technique. Mass spectral analysis revealed the presence of two metabolites viz., octocrylene and squalene in the toxin fraction.

Original languageEnglish
Pages (from-to)543-546
Number of pages4
JournalAustralasian Plant Pathology
Volume47
Issue number5
DOIs
Publication statusPublished - Sep 1 2018

Fingerprint

Monosporascus cannonballus
decay fungi
culture filtrates
root rot
melons
toxins
cucumbers
electrolytes
vigor
leaves
metabolites
squalene
phytotoxins
seedlings
assays
phytotoxicity
dialysis
thin layer chromatography
chloroform
spectral analysis

Keywords

  • Melon
  • Monosporascus cannonballus
  • Root rot
  • Toxin

ASJC Scopus subject areas

  • Plant Science

Cite this

Toxin production by melon root rot fungus, Monosporascus cannonballus. / Al-Rawahi, Amna Yaqoob; Al-Mahmooli, Issa Hashil; Al-Sadi, Abdullah; Al-Sabahi, Jamal Nasser; Rethinasamy, Velazhahan.

In: Australasian Plant Pathology, Vol. 47, No. 5, 01.09.2018, p. 543-546.

Research output: Contribution to journalArticle

Al-Rawahi, Amna Yaqoob ; Al-Mahmooli, Issa Hashil ; Al-Sadi, Abdullah ; Al-Sabahi, Jamal Nasser ; Rethinasamy, Velazhahan. / Toxin production by melon root rot fungus, Monosporascus cannonballus. In: Australasian Plant Pathology. 2018 ; Vol. 47, No. 5. pp. 543-546.
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