TY - JOUR
T1 - Toxic effects of Litsea elliptica Blume essential oil on red blood cells of Sprague-Dawley rats
AU - Taib, Izatus Shima
AU - Budin, Siti Balkis
AU - Siti Nor Ain, Seri Maseran
AU - Mohamed, Jamaludin
AU - Louis, Santhana Raj
AU - Das, Srijit
AU - Sallehudin, Sulaiman
AU - Rajab, Nor Fadilah
AU - Hidayatulfathi, Othman
N1 - Funding Information:
‡ Corresponding author * Project (No. 02-01-02-SF0205) supported by the Ministry of Science, Technology and Innovation of Malaysia
PY - 2009
Y1 - 2009
N2 - Litsea elliptica Blume leaves have been traditionally used as medicinal herbs because of its antimutagenicity, chemopreventative and insecticidal properties. In this study, the toxic effects of L. elliptica essential oil against Sprague-Dawley rat's red blood cells (RBCs) were evaluated. L. elliptica essential oil was given by oral gavage 5 times per week for 3 treated groups in the doses of 125, 250, and 500 mg/(kg body weight), respectively, and the control group received distilled water. Full blood count, RBC osmotic fragility, RBC morphological changes, and RBC membrane lipid were analyzed 28 d after the treatment. Although L. elliptica essential oil administration had significantly different effects on hemoglobin (Hb), mean cell hemoglobin concentration (MCHC), mean cell volume (MCV), and mean cell hemoglobin (MCH) in the experimental groups as compared to the control group (P<0.05), the values were still within the normal range. L. elliptica induced morphological changes of RBC into the form of echinocyte. The percentage of echinocyte increased significantly among the treated groups in a dose-response manner (P<0.001). The concentrations of RBC membrane phospholipids and cholesterol of all treated groups were significantly lower than those of control group (P<0.001). However, the RBC membrane osmotic fragility and total proteins of RBC membrane findings did not differ significantly between control and treated groups (P>0.05). It is concluded that structural changes in the RBC membrane due to L. elliptica essential oil administration did not cause severe membrane damage.
AB - Litsea elliptica Blume leaves have been traditionally used as medicinal herbs because of its antimutagenicity, chemopreventative and insecticidal properties. In this study, the toxic effects of L. elliptica essential oil against Sprague-Dawley rat's red blood cells (RBCs) were evaluated. L. elliptica essential oil was given by oral gavage 5 times per week for 3 treated groups in the doses of 125, 250, and 500 mg/(kg body weight), respectively, and the control group received distilled water. Full blood count, RBC osmotic fragility, RBC morphological changes, and RBC membrane lipid were analyzed 28 d after the treatment. Although L. elliptica essential oil administration had significantly different effects on hemoglobin (Hb), mean cell hemoglobin concentration (MCHC), mean cell volume (MCV), and mean cell hemoglobin (MCH) in the experimental groups as compared to the control group (P<0.05), the values were still within the normal range. L. elliptica induced morphological changes of RBC into the form of echinocyte. The percentage of echinocyte increased significantly among the treated groups in a dose-response manner (P<0.001). The concentrations of RBC membrane phospholipids and cholesterol of all treated groups were significantly lower than those of control group (P<0.001). However, the RBC membrane osmotic fragility and total proteins of RBC membrane findings did not differ significantly between control and treated groups (P>0.05). It is concluded that structural changes in the RBC membrane due to L. elliptica essential oil administration did not cause severe membrane damage.
KW - Cell membrane
KW - Echinocyte
KW - Litsea elliptica
KW - Morphology
KW - Red blood cells
KW - Toxicity
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U2 - 10.1631/jzus.B0920199
DO - 10.1631/jzus.B0920199
M3 - Article
C2 - 19882755
AN - SCOPUS:73449143840
SN - 1673-1581
VL - 10
SP - 813
EP - 819
JO - Journal of Zhejiang University: Science B
JF - Journal of Zhejiang University: Science B
IS - 11
ER -