TY - JOUR
T1 - Time-course effects of systemically administered diesel exhaust particles in rats
AU - Nemmar, Abderrahim
AU - Al-Salam, Suhail
AU - Zia, Shaheen
AU - Dhanasekaran, Subramanian
AU - Shudadevi, Munjusha
AU - Ali, Badreldin H.
N1 - Funding Information:
The authors wish to thank Ms. Hamda Ahmad Kamalboor and Ms. Shamma Ahmed Al-Ali for their technical help. This work was supported by funds of the Faculty of Medicine and Health Sciences, United Arab Emirates University grant (Grant number: NP/09/04 ), and the United Arab Emirates individual grant ( 01_43_08_11/09 ).
PY - 2010/5
Y1 - 2010/5
N2 - Nanosized fraction of particulate air pollution has been reported to translocate from the airways into the bloodstream and act on different organs. However, the direct effect of these translocated particles is not well understood. In this study, we determined the time-course (6h, 18. h, 48. h and 168. h) effects of the systemic administration of 0.02. mg/kg diesel exhaust particles (DEP) on systolic blood pressure (SBP), systemic inflammation, oxidative status, and morphological alterations in lungs, heart, liver and kidneys in Wistar rats. SBP was significantly decreased at 6. h (P<0.05) but no significant effects have been observed at later time points. The leukocyte numbers were increased at 6. h (P<0.05) and 18. h (P<0.05). However, the platelet numbers were significantly decreased (P<0.05) 6. h following the systemic administration of DEP. The IL-6 concentrations in plasma was increased at 6. h (P<0.05) and 18. h (P<0.05). Similarly, superoxide dismutase activity was significantly increased at 6 (P=0.01) and 18. h (P<0.05) following DEP exposure. The direct addition of DEP (0.1-1μg/ml) to untreated rat blood significantly induced in vitro platelet aggregation in a dose-dependent fashion. The activation of intravascular coagulation was confirmed by a dose-dependent shortening of activated partial thromboplastin time and the prothrombin time following in vitro exposure to DEP (0.25-1μg/ml). Histological analysis revealed the presence of DEP in the lungs, heart, liver and kidneys. However, the morphological changes were only observed in the lungs, where the presence of infiltration of inflammatory cells was observed as early as 6. h, increased at 18. h, and decreased in intensity at 48. h and at 168. h. We conclude that the direct systemic administration of DEP caused acute effect on SBP (6. h) and systemic inflammation and oxidative stress mainly at 6. h and 18. h. Despite the presence of DEP in lungs, heart, liver and kidneys, the histopathological changes were only seen in the lung which suggests that, at the dose and time-points investigated, DEP cause inflammation and have a predilection for pulmonary tissue.
AB - Nanosized fraction of particulate air pollution has been reported to translocate from the airways into the bloodstream and act on different organs. However, the direct effect of these translocated particles is not well understood. In this study, we determined the time-course (6h, 18. h, 48. h and 168. h) effects of the systemic administration of 0.02. mg/kg diesel exhaust particles (DEP) on systolic blood pressure (SBP), systemic inflammation, oxidative status, and morphological alterations in lungs, heart, liver and kidneys in Wistar rats. SBP was significantly decreased at 6. h (P<0.05) but no significant effects have been observed at later time points. The leukocyte numbers were increased at 6. h (P<0.05) and 18. h (P<0.05). However, the platelet numbers were significantly decreased (P<0.05) 6. h following the systemic administration of DEP. The IL-6 concentrations in plasma was increased at 6. h (P<0.05) and 18. h (P<0.05). Similarly, superoxide dismutase activity was significantly increased at 6 (P=0.01) and 18. h (P<0.05) following DEP exposure. The direct addition of DEP (0.1-1μg/ml) to untreated rat blood significantly induced in vitro platelet aggregation in a dose-dependent fashion. The activation of intravascular coagulation was confirmed by a dose-dependent shortening of activated partial thromboplastin time and the prothrombin time following in vitro exposure to DEP (0.25-1μg/ml). Histological analysis revealed the presence of DEP in the lungs, heart, liver and kidneys. However, the morphological changes were only observed in the lungs, where the presence of infiltration of inflammatory cells was observed as early as 6. h, increased at 18. h, and decreased in intensity at 48. h and at 168. h. We conclude that the direct systemic administration of DEP caused acute effect on SBP (6. h) and systemic inflammation and oxidative stress mainly at 6. h and 18. h. Despite the presence of DEP in lungs, heart, liver and kidneys, the histopathological changes were only seen in the lung which suggests that, at the dose and time-points investigated, DEP cause inflammation and have a predilection for pulmonary tissue.
KW - Diesel exhaust particles
KW - Lung inflammation
KW - Rats
KW - Systemic inflammation
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U2 - 10.1016/j.toxlet.2010.02.001
DO - 10.1016/j.toxlet.2010.02.001
M3 - Article
C2 - 20144906
AN - SCOPUS:77950857865
SN - 0378-4274
VL - 194
SP - 58
EP - 65
JO - Toxicology Letters
JF - Toxicology Letters
IS - 3
ER -