Theileria lestoquardi displays reduced genetic diversity relative to sympatric Theileria annulata in Oman

Salama Al-Hamidhi, William Weir, Jane Kinnaird, Mohemmed Tageledin, Albano Beja-Pereira, Ivan Morrison, Joanne Thompson, Andy Tait, Brian Shiels, Hamza A. Babiker

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The Apicomplexan parasites, Theileria lestoquardi and Theileria annulata, the causative agents of theileriosis in small and large ruminants, are widespread in Oman, in areas where cattle, sheep and goats co-graze. Genetic analysis can provide insight into the dynamics of the parasite and the evolutionary relationship between species. Here we identified ten genetic markers (micro- and mini-satellites) spread across the T. lestoquardi genome, and confirmed their species specificity. We then genotyped T. lestoquardi in different regions in Oman. The genetic structures of T. lestoquardi populations were then compared with previously published data, for comparable panels of markers, for sympatric T. annulata isolates. In addition, we examined two antigen genes in T. annulata (Tams1 and Ta9) and their orthologues in T. lestoquardi (Tlms1 and Tl9).The genetic diversity and multiplicity of infection (MOI) were lower in T. lestoquardi (He = 0.64-0.77) than T. annulata (He = 0.83-0.85) in all populations. Very limited genetic differentiation was found among T. lestoquardi and T. annulata populations. In contrast, limited but significant linkage disequilibrium was observed within regional populations of each species. We identified eight T. annulata isolates in small ruminants; the diversity and MOI were lower among ovine/caprine compared to bovine. Sequence diversity of the antigen genes, Tams1 and Ta9 in T. annulata (π = 0.0733 and π = 0.155 respectively), was 10-fold and 3-fold higher than the orthologous Tlms1 and Tl9 in T. lestoquardi (π = 0.006 and π = 0.055, respectively).Despite a comparably high prevalence, T. lestoquardi has lower genetic diversity compared to sympatric T. annulata populations. There was no evidence of differentiation among populations of either species. In comparison to T. lestoquardi, T. annulata has a larger effective population size. While genetic exchange and recombination occur in both parasite species, the extent of diversity, overall, is less for T. lestoquardi. It is, therefore, likely that T. lestoquardi evolved from an ancestor of present day T. annulata and that this occurred either once or on a limited number of occasions.

Original languageEnglish
Pages (from-to)297-306
Number of pages10
JournalInfection, Genetics and Evolution
Volume43
DOIs
Publication statusPublished - Sep 1 2016

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Theileria annulata
Theileria
Oman
genetic variation
parasite
ruminant
antigen
Population
fold
Parasites
gene
effective population size
Ruminants
genetic marker
genetic analysis
goat
parasites
genetic differentiation
ancestry
disequilibrium

Keywords

  • Evolution
  • Host species jump
  • Oman
  • Population genetics
  • Theileria annulata
  • Theileria lestoquardi

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Molecular Biology
  • Microbiology
  • Infectious Diseases
  • Microbiology (medical)

Cite this

Theileria lestoquardi displays reduced genetic diversity relative to sympatric Theileria annulata in Oman. / Al-Hamidhi, Salama; Weir, William; Kinnaird, Jane; Tageledin, Mohemmed; Beja-Pereira, Albano; Morrison, Ivan; Thompson, Joanne; Tait, Andy; Shiels, Brian; Babiker, Hamza A.

In: Infection, Genetics and Evolution, Vol. 43, 01.09.2016, p. 297-306.

Research output: Contribution to journalArticle

Al-Hamidhi, S, Weir, W, Kinnaird, J, Tageledin, M, Beja-Pereira, A, Morrison, I, Thompson, J, Tait, A, Shiels, B & Babiker, HA 2016, 'Theileria lestoquardi displays reduced genetic diversity relative to sympatric Theileria annulata in Oman', Infection, Genetics and Evolution, vol. 43, pp. 297-306. https://doi.org/10.1016/j.meegid.2016.05.007
Al-Hamidhi, Salama ; Weir, William ; Kinnaird, Jane ; Tageledin, Mohemmed ; Beja-Pereira, Albano ; Morrison, Ivan ; Thompson, Joanne ; Tait, Andy ; Shiels, Brian ; Babiker, Hamza A. / Theileria lestoquardi displays reduced genetic diversity relative to sympatric Theileria annulata in Oman. In: Infection, Genetics and Evolution. 2016 ; Vol. 43. pp. 297-306.
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