The VNTR polymorphism in the human dopamine transporter gene

Improved detection and absence of association of VNTR alleles with attention-deficit hyperactivity disorder

Mehmet Simsek, Marwan Al-Sharbati, Samir Al-Adawi, Kholuud Lawatia

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The human dopamine transporter (DAT1) gene contains a variable number tandem repeat (VNTR) in its 3′-untranslated region because of repetition of a 40-bp core sequence. Methods available for the diagnosis of this polymorphism are limited in number. We have developed a new polymerase chain reaction (PCR) test, which is similar to that described originally by Vandenbergh's group, but provides a better detection of the VNTR alleles in the human DAT1 gene. Using two independent PCR methods, we have determined the distribution of VNTR alleles in 110 healthy Omani subjects, and in 92 children with attention-deficit hyperactivity disorder (ADHD). The frequency of the risk allele (DAT1*10) was similar in the healthy subjects and ADHD cases, indicating absence of association of this allele with ADHD in Oman.

Original languageEnglish
Pages (from-to)31-34
Number of pages4
JournalGenetic Testing
Volume10
Issue number1
DOIs
Publication statusPublished - Mar 2006

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Minisatellite Repeats
Dopamine Plasma Membrane Transport Proteins
Attention Deficit Disorder with Hyperactivity
Alleles
Healthy Volunteers
Oman
Genes
Polymerase Chain Reaction
3' Untranslated Regions
Gene Frequency

ASJC Scopus subject areas

  • Genetics(clinical)

Cite this

The VNTR polymorphism in the human dopamine transporter gene : Improved detection and absence of association of VNTR alleles with attention-deficit hyperactivity disorder. / Simsek, Mehmet; Al-Sharbati, Marwan; Al-Adawi, Samir; Lawatia, Kholuud.

In: Genetic Testing, Vol. 10, No. 1, 03.2006, p. 31-34.

Research output: Contribution to journalArticle

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