The prevalence of dengue virus in Brunei Darussalam during January-November 2010.

Zaharuddin H A Rahman, Osmali Osman, Siti Hanna Muharram, Mohamed Mabruk

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The aim of this study was to retrospectively determine the prevalence of dengue virus from April 2010 to November 2010 in Brunei Darussalam. A total of 250 serum samples from dengue diagnosed patients were examined. All serum samples were tested for dengue IgM and IgG antibodies and dengue NS1 antigen using the PanBio dengue ELISA commercial kit. To determine the prevalence of dengue virus serotype in the country, serotyping was performed for the 14 samples that were positive for NS1 antigen. Dengue virus serotyping was carried out using the conventional reverse transcriptase-polymerase chain reaction (RT-PCR). Of the 250 serum samples included in the study, 196 were laboratory dengue confirmed cases. Dengue virus serotype 1 (DENV-1) was the predominant circulating serotype, followed by DENV-3 and DENV-2. This is the first report of DENV-3 isolation in Brunei Darussalam.

Original languageEnglish
Pages (from-to)594-601
Number of pages8
JournalThe Southeast Asian journal of tropical medicine and public health
Volume44
Issue number4
Publication statusPublished - Jul 4 2013

Fingerprint

Brunei
Dengue Virus
Dengue
Serotyping
Serum
Antigens
Reverse Transcriptase Polymerase Chain Reaction
Immunoglobulin M
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay
Antibodies
Serogroup

ASJC Scopus subject areas

  • Infectious Diseases
  • Public Health, Environmental and Occupational Health

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The prevalence of dengue virus in Brunei Darussalam during January-November 2010. / Rahman, Zaharuddin H A; Osman, Osmali; Muharram, Siti Hanna; Mabruk, Mohamed.

In: The Southeast Asian journal of tropical medicine and public health, Vol. 44, No. 4, 04.07.2013, p. 594-601.

Research output: Contribution to journalArticle

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