The parasitic trematode Fasciola hepatica exhibits mammalian-type glycolipids as well as Gal(Β1-6)Gal-terminating glycolipids that account for cestode serological cross-reactivity

Manfred Wuhrer, Christiane Grimm, Roger D. Dennis, Mohamed A. Idris, Rudolf Geyer

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Neutral glycosphingolipids from sheep-derived Fasciola hepatica liver flukes were isolated and characterized both structurally and serologically. After HPLC fractionation, glycolipids were analyzed by linkage analysis, enzymatic cleavage, and MALDI-TOF as well as electrospray ionization mass spectrometry. Obtained results revealed the presence of two types of neutral glycolipids. The first group represented mammalian-type species comprising globo- and isoglobotriaosylceramides (Gal(α1-4)Gal(β1-4)Glc(1-1)ceramide and Gal(α1-3)Gal(β1-4)Glc(1-1)ceramide, respectively) as well as Forssman antigen (GalNAc(α1-3)GalNAc(β1-3/4)Gal(α1-4/3)Gal(β1-4 Glc(1-1)ceramide). Applying Helix pomatia agglutinin, recognizing terminal α-linked GalNAc, to cryosections of adult flukes, the latter glycolipid could be localized to the F. hepatica gut. As Forssman antigen from the parasite and sheep host led to identical MALDI-TOF MS profiles, this glycolipid might be acquired from the definitive host. As a second group, highly antigenic glycolipids were structurally characterized as Gal(β1-6)Gal(β1-4)Glc(1-1)ceramide, Gal(β1-6)Gal(α1-3/4)Gal(β1-4)Glc(1-1)ceramide and Gal(β1-6)Gal(β1-6)Gal(α1-3/4)Gal(β1-4)Glc(1-1 ceramide, the latter two structures of which exhibited both isoglobo- or globo-series core structures. Terminal Gal(β1-6)Gal1- motifs have previously been shown to represent antigenic epitopes of neogala-series glycosphingolipids from tape worms. Using human Echinococcus granulosus infection sera, Gal(β1-6)-Gal-terminating glycolipids could be allocated to the gut in adult liver fluke cryosections. Corresponding neogala-reactive antibodies in F. hepatica infection serum were detected by their binding to E. granulosus and Taenia crassiceps neogalaglycosphingolipids. These antibodies might contribute to the known serological cross-reactivity between F. hepatica and parasitic cestode infections.

Original languageEnglish
Pages (from-to)115-126
Number of pages12
JournalGlycobiology
Volume14
Issue number2
DOIs
Publication statusPublished - Feb 2004

Fingerprint

Fasciola hepatica
Cestoda
Ceramides
Glycolipids
Forssman Antigen
Echinococcus granulosus
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Liver
Sheep
Neutral Glycosphingolipids
Cestode Infections
Taenia
Electrospray ionization
Trematoda
Glycosphingolipids
Parasitic Diseases
Electrospray Ionization Mass Spectrometry
Antibodies
Fractionation
Infection

Keywords

  • Forssman antigen
  • Liver-fluke glycolipids
  • Neogala-series glycolipids
  • Oligosaccharide structural analysis
  • Parasitic trematode

ASJC Scopus subject areas

  • Biochemistry

Cite this

The parasitic trematode Fasciola hepatica exhibits mammalian-type glycolipids as well as Gal(Β1-6)Gal-terminating glycolipids that account for cestode serological cross-reactivity. / Wuhrer, Manfred; Grimm, Christiane; Dennis, Roger D.; Idris, Mohamed A.; Geyer, Rudolf.

In: Glycobiology, Vol. 14, No. 2, 02.2004, p. 115-126.

Research output: Contribution to journalArticle

@article{0ec4785b52db4187b6c3bce335e54b09,
title = "The parasitic trematode Fasciola hepatica exhibits mammalian-type glycolipids as well as Gal(Β1-6)Gal-terminating glycolipids that account for cestode serological cross-reactivity",
abstract = "Neutral glycosphingolipids from sheep-derived Fasciola hepatica liver flukes were isolated and characterized both structurally and serologically. After HPLC fractionation, glycolipids were analyzed by linkage analysis, enzymatic cleavage, and MALDI-TOF as well as electrospray ionization mass spectrometry. Obtained results revealed the presence of two types of neutral glycolipids. The first group represented mammalian-type species comprising globo- and isoglobotriaosylceramides (Gal(α1-4)Gal(β1-4)Glc(1-1)ceramide and Gal(α1-3)Gal(β1-4)Glc(1-1)ceramide, respectively) as well as Forssman antigen (GalNAc(α1-3)GalNAc(β1-3/4)Gal(α1-4/3)Gal(β1-4 Glc(1-1)ceramide). Applying Helix pomatia agglutinin, recognizing terminal α-linked GalNAc, to cryosections of adult flukes, the latter glycolipid could be localized to the F. hepatica gut. As Forssman antigen from the parasite and sheep host led to identical MALDI-TOF MS profiles, this glycolipid might be acquired from the definitive host. As a second group, highly antigenic glycolipids were structurally characterized as Gal(β1-6)Gal(β1-4)Glc(1-1)ceramide, Gal(β1-6)Gal(α1-3/4)Gal(β1-4)Glc(1-1)ceramide and Gal(β1-6)Gal(β1-6)Gal(α1-3/4)Gal(β1-4)Glc(1-1 ceramide, the latter two structures of which exhibited both isoglobo- or globo-series core structures. Terminal Gal(β1-6)Gal1- motifs have previously been shown to represent antigenic epitopes of neogala-series glycosphingolipids from tape worms. Using human Echinococcus granulosus infection sera, Gal(β1-6)-Gal-terminating glycolipids could be allocated to the gut in adult liver fluke cryosections. Corresponding neogala-reactive antibodies in F. hepatica infection serum were detected by their binding to E. granulosus and Taenia crassiceps neogalaglycosphingolipids. These antibodies might contribute to the known serological cross-reactivity between F. hepatica and parasitic cestode infections.",
keywords = "Forssman antigen, Liver-fluke glycolipids, Neogala-series glycolipids, Oligosaccharide structural analysis, Parasitic trematode",
author = "Manfred Wuhrer and Christiane Grimm and Dennis, {Roger D.} and Idris, {Mohamed A.} and Rudolf Geyer",
year = "2004",
month = "2",
doi = "10.1093/glycob/cwh021",
language = "English",
volume = "14",
pages = "115--126",
journal = "Glycobiology",
issn = "0959-6658",
publisher = "Oxford University Press",
number = "2",

}

TY - JOUR

T1 - The parasitic trematode Fasciola hepatica exhibits mammalian-type glycolipids as well as Gal(Β1-6)Gal-terminating glycolipids that account for cestode serological cross-reactivity

AU - Wuhrer, Manfred

AU - Grimm, Christiane

AU - Dennis, Roger D.

AU - Idris, Mohamed A.

AU - Geyer, Rudolf

PY - 2004/2

Y1 - 2004/2

N2 - Neutral glycosphingolipids from sheep-derived Fasciola hepatica liver flukes were isolated and characterized both structurally and serologically. After HPLC fractionation, glycolipids were analyzed by linkage analysis, enzymatic cleavage, and MALDI-TOF as well as electrospray ionization mass spectrometry. Obtained results revealed the presence of two types of neutral glycolipids. The first group represented mammalian-type species comprising globo- and isoglobotriaosylceramides (Gal(α1-4)Gal(β1-4)Glc(1-1)ceramide and Gal(α1-3)Gal(β1-4)Glc(1-1)ceramide, respectively) as well as Forssman antigen (GalNAc(α1-3)GalNAc(β1-3/4)Gal(α1-4/3)Gal(β1-4 Glc(1-1)ceramide). Applying Helix pomatia agglutinin, recognizing terminal α-linked GalNAc, to cryosections of adult flukes, the latter glycolipid could be localized to the F. hepatica gut. As Forssman antigen from the parasite and sheep host led to identical MALDI-TOF MS profiles, this glycolipid might be acquired from the definitive host. As a second group, highly antigenic glycolipids were structurally characterized as Gal(β1-6)Gal(β1-4)Glc(1-1)ceramide, Gal(β1-6)Gal(α1-3/4)Gal(β1-4)Glc(1-1)ceramide and Gal(β1-6)Gal(β1-6)Gal(α1-3/4)Gal(β1-4)Glc(1-1 ceramide, the latter two structures of which exhibited both isoglobo- or globo-series core structures. Terminal Gal(β1-6)Gal1- motifs have previously been shown to represent antigenic epitopes of neogala-series glycosphingolipids from tape worms. Using human Echinococcus granulosus infection sera, Gal(β1-6)-Gal-terminating glycolipids could be allocated to the gut in adult liver fluke cryosections. Corresponding neogala-reactive antibodies in F. hepatica infection serum were detected by their binding to E. granulosus and Taenia crassiceps neogalaglycosphingolipids. These antibodies might contribute to the known serological cross-reactivity between F. hepatica and parasitic cestode infections.

AB - Neutral glycosphingolipids from sheep-derived Fasciola hepatica liver flukes were isolated and characterized both structurally and serologically. After HPLC fractionation, glycolipids were analyzed by linkage analysis, enzymatic cleavage, and MALDI-TOF as well as electrospray ionization mass spectrometry. Obtained results revealed the presence of two types of neutral glycolipids. The first group represented mammalian-type species comprising globo- and isoglobotriaosylceramides (Gal(α1-4)Gal(β1-4)Glc(1-1)ceramide and Gal(α1-3)Gal(β1-4)Glc(1-1)ceramide, respectively) as well as Forssman antigen (GalNAc(α1-3)GalNAc(β1-3/4)Gal(α1-4/3)Gal(β1-4 Glc(1-1)ceramide). Applying Helix pomatia agglutinin, recognizing terminal α-linked GalNAc, to cryosections of adult flukes, the latter glycolipid could be localized to the F. hepatica gut. As Forssman antigen from the parasite and sheep host led to identical MALDI-TOF MS profiles, this glycolipid might be acquired from the definitive host. As a second group, highly antigenic glycolipids were structurally characterized as Gal(β1-6)Gal(β1-4)Glc(1-1)ceramide, Gal(β1-6)Gal(α1-3/4)Gal(β1-4)Glc(1-1)ceramide and Gal(β1-6)Gal(β1-6)Gal(α1-3/4)Gal(β1-4)Glc(1-1 ceramide, the latter two structures of which exhibited both isoglobo- or globo-series core structures. Terminal Gal(β1-6)Gal1- motifs have previously been shown to represent antigenic epitopes of neogala-series glycosphingolipids from tape worms. Using human Echinococcus granulosus infection sera, Gal(β1-6)-Gal-terminating glycolipids could be allocated to the gut in adult liver fluke cryosections. Corresponding neogala-reactive antibodies in F. hepatica infection serum were detected by their binding to E. granulosus and Taenia crassiceps neogalaglycosphingolipids. These antibodies might contribute to the known serological cross-reactivity between F. hepatica and parasitic cestode infections.

KW - Forssman antigen

KW - Liver-fluke glycolipids

KW - Neogala-series glycolipids

KW - Oligosaccharide structural analysis

KW - Parasitic trematode

UR - http://www.scopus.com/inward/record.url?scp=1342263739&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1342263739&partnerID=8YFLogxK

U2 - 10.1093/glycob/cwh021

DO - 10.1093/glycob/cwh021

M3 - Article

VL - 14

SP - 115

EP - 126

JO - Glycobiology

JF - Glycobiology

SN - 0959-6658

IS - 2

ER -