The influence of dissolved organic matter (DOM) on sodium regulation and nitrogenous waste excretion in the zebrafish (Danio rerio)

Hassan A. Al-Reasi, Scott D. Smith, Chris M. Wood

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Dissolved organic matter (DOM) is both ubiquitous and diverse in composition in natural waters, but its effects on the branchial physiology of aquatic organisms have received little attention relative to other variables (e.g. pH, hardness, salinity, alkalinity). Here, we investigated the effects of four chemically distinct DOM isolates (three natural, one commercial, ranging from autochthonous to highly allochthonous, all at ∼6 mg C l-1) on the physiology of gill ionoregulation and nitrogenous waste excretion in zebrafish acclimated to either circumneutral (7.0-8.0) or acidic pH (5.0). Overall, lower pH tended to increase net branchial ammonia excretion, net K+ loss and [3H]PEG-4000 clearance rates (indicators of transcellular and paracellular permeability, respectively). However, unidirectional Na+ efflux, urea excretion and drinking rates were unaffected. DOM sources tended to stimulate unidirectional Na+ influx rate and exerted subtle effects on the concentration-dependent kinetics of Na+ uptake, increasing maximum transport capacity. All DOM sources reduced passive Na+ efflux rates regardless of pH, but exerted negligible effects on nitrogenous waste excretion, drinking rate, net K+ loss or [3H]PEG-4000 clearance, so the mechanism of Na+ loss reduction remains unclear. Overall, these actions appear beneficial to ionoregulatory homeostasis in zebrafish, and some may be related to physicochemical properties of the DOM sources. They are very different from those seen in a recent parallel study on Daphnia magna using the sameDOMisolates, indicating thatDOMactions may be both species and DOM specific.

Original languageEnglish
Pages (from-to)2289-2299
Number of pages11
JournalJournal of Experimental Biology
Volume219
Issue number15
DOIs
Publication statusPublished - Aug 1 2016

Fingerprint

dissolved organic matter
Zebrafish
Danio rerio
excretion
Sodium
sodium
Drinking
drinking
Daphnia
Aquatic Organisms
physiology
Salinity
Hardness
Ammonia
Urea
Permeability
clearance rate
Homeostasis
Daphnia magna
homeostasis

Keywords

  • Ammonia fluxes
  • DOC
  • Drinking rate
  • K+ fluxes
  • Na+ fluxes
  • Urea fluxes

ASJC Scopus subject areas

  • Medicine(all)
  • Physiology
  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science
  • Molecular Biology
  • Animal Science and Zoology
  • Insect Science

Cite this

The influence of dissolved organic matter (DOM) on sodium regulation and nitrogenous waste excretion in the zebrafish (Danio rerio). / Al-Reasi, Hassan A.; Smith, Scott D.; Wood, Chris M.

In: Journal of Experimental Biology, Vol. 219, No. 15, 01.08.2016, p. 2289-2299.

Research output: Contribution to journalArticle

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