TY - JOUR
T1 - The efficacy of selected plant extracts and bioflavonoids in controlling infections of Saprolegnia australis (Saprolegniales; Oomycetes)
AU - Caruana, S.
AU - Yoon, G. H.
AU - Freeman, M. A.
AU - Mackie, J. A.
AU - Shinn, A. P.
N1 - Funding Information:
The financial support provided by SAAS to SC is greatly appreciated and University of Malaya grant UMC/625/1/HIR/027 . The authors would like to thank Dr Andrew Tildesley and Mrs. Gillian Dreczkowski from the Institute of Aquaculture for assistance with the technical aspects of this study.
PY - 2012/8/15
Y1 - 2012/8/15
N2 - Saprolegnia infections can account for the significant loss of salmonid eggs and adults in breeding condition and although there are a number of chemical agents available to control infections in hatcheries, treatment is difficult and there are reservations regarding their efficacy, prompting a search for suitable replacements. Here, the efficacy of 24 crude plant extracts and 3 bioflavonoids on the growth of . Saprolegnia australis were assessed using an in vitro agar diffusion method against a set of synthetic chemotherapeutants commonly used within the aquaculture industry. The 12 best extracts inhibiting growth at ≤. 100. ppm, were then added to cultures of . S. . australis in a GP broth and the daily growth was monitored spectrophotometrically over a 7-day period. All 12 plant extracts reduced growth, however, only . Atractylodes macrocephala, . Zingiber officinale seed, . Chrysanthemum and . Yucca were as effective as 10. ppm malachite green in slowing growth. A subsequent trial, where doses of the plant extract were given at 72 and 120. h post-inoculation, found that . Rumex obtusifolius, . Sophora flavescens, . Echinacea and . Z. . officinale were the most effective compounds impacting growth. The study demonstrates that certain plant extracts impact on the growth dynamics of . S. . australis but further research is required to assess their performance in vivo.
AB - Saprolegnia infections can account for the significant loss of salmonid eggs and adults in breeding condition and although there are a number of chemical agents available to control infections in hatcheries, treatment is difficult and there are reservations regarding their efficacy, prompting a search for suitable replacements. Here, the efficacy of 24 crude plant extracts and 3 bioflavonoids on the growth of . Saprolegnia australis were assessed using an in vitro agar diffusion method against a set of synthetic chemotherapeutants commonly used within the aquaculture industry. The 12 best extracts inhibiting growth at ≤. 100. ppm, were then added to cultures of . S. . australis in a GP broth and the daily growth was monitored spectrophotometrically over a 7-day period. All 12 plant extracts reduced growth, however, only . Atractylodes macrocephala, . Zingiber officinale seed, . Chrysanthemum and . Yucca were as effective as 10. ppm malachite green in slowing growth. A subsequent trial, where doses of the plant extract were given at 72 and 120. h post-inoculation, found that . Rumex obtusifolius, . Sophora flavescens, . Echinacea and . Z. . officinale were the most effective compounds impacting growth. The study demonstrates that certain plant extracts impact on the growth dynamics of . S. . australis but further research is required to assess their performance in vivo.
KW - Antifungal activity
KW - Fish pathogen
KW - Fungal infection
KW - Salmonids
KW - Water mould
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U2 - 10.1016/j.aquaculture.2012.06.035
DO - 10.1016/j.aquaculture.2012.06.035
M3 - Article
AN - SCOPUS:84864046665
SN - 0044-8486
VL - 358-359
SP - 146
EP - 154
JO - Aquaculture
JF - Aquaculture
ER -