The effect of substratum type, orientation and depth on the development of bacterial deep-sea biofilm communities grown on artificial substrata deployed in the Eastern Mediterranean

Nikoleta Bellou, Evangelos Papathanassiou, Sergey Dobretsov, Vassilis Lykousis, Franciscus Colijn

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

An increasing number of deep-sea studies have highlighted the importance of deep-sea biofouling, especially in relation to the protection of deep-sea instruments. In this study, the microbial communities developed on different substrata (titanium, aluminum, limestone, shale and neutrino telescope glass) exposed for 155 days at different depths (1500 m, 2500 m, 3500 m and 4500 m) and positions (vertical and horizontal) in the Eastern Mediterranean Deep Sea were compared. Replicated biofilm samples were analyzed using a Terminal Restriction Fragment Length Polymorphisms (T-RFLP) method. The restriction enzymes CfoI and RsaI produced similar total numbers (94, 93) of different T-RFLP peaks (T-RFs) along the vertical transect. In contrast, the mean total T-RF number between each sample according to substratum type and depth was higher in more samples when CfoI was used. The total species richness (S) of the bacterial communities differed significantly between the substrata, and depended on the orientation of each substratum within one depth and throughout the water column (ANOVA). T-RFLP analyses using the Jaccard similarity index showed that within one depth layer, the composition of microbial communities on different substrata was different and highly altered among communities developed on the same substratum but exposed to fouling at different depths. Based on Multidimensional Scaling Analyses (MDS), the study suggests that depth plays an important role in the composition of deep-sea biofouling communities, while substratum type and orientation of substrata throughout the water column are less important. To the authors' knowledge, this is the first study of biofilm development in deep waters, in relation to the effects of substratum type, orientation and depth.

Original languageEnglish
Pages (from-to)199-213
Number of pages15
JournalBiofouling
Volume28
Issue number2
DOIs
Publication statusPublished - Feb 2012

Fingerprint

Biofilms
Oceans and Seas
Restriction Fragment Length Polymorphisms
biofilm
deep sea
Biofouling
restriction fragment length polymorphism
Water
biofouling
Mediterranean Sea
polymorphism
Telescopes
Calcium Carbonate
Titanium
microbial communities
Aluminum
microbial community
Glass
water column
Analysis of Variance

Keywords

  • artificial substratum
  • bacteria
  • biofilm
  • deep-sea
  • fouling
  • Mediterranean Sea

ASJC Scopus subject areas

  • Aquatic Science
  • Water Science and Technology
  • Applied Microbiology and Biotechnology

Cite this

The effect of substratum type, orientation and depth on the development of bacterial deep-sea biofilm communities grown on artificial substrata deployed in the Eastern Mediterranean. / Bellou, Nikoleta; Papathanassiou, Evangelos; Dobretsov, Sergey; Lykousis, Vassilis; Colijn, Franciscus.

In: Biofouling, Vol. 28, No. 2, 02.2012, p. 199-213.

Research output: Contribution to journalArticle

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