TY - JOUR
T1 - The Association of Human Leukocyte Antigens Complex with Type 1 Diabetes in the Omani Population
AU - Al-Balushi, Mohammed
AU - Al-Badi, Samiya
AU - Al-Yaarubi, Saif
AU - Al-Riyami, Hamad
AU - Al-Shidhani, Azza
AU - Al-Hinai, Shaima
AU - Alshirawi, Ali
AU - Hasson, Sidgi
AU - Said, Elias
AU - Al-Jabri, Ali
AU - Al Ansari, Aliya
N1 - Funding Information:
This work was supported by The Research Council fund, Oman (RClMEDlMICRl14l01).
Publisher Copyright:
© 2023, Sultan Qaboos University. All rights reserved.
© Copyright 2023, Sultan Qaboos University Medical Journal, All Rights Reserved.
PY - 2023/2/23
Y1 - 2023/2/23
N2 - OBJECTIVES: Identification of the high risk alleles, genotypes and haplotypes of the human leukocyte antigens (HLA) in different populations is beneficial for understanding their roles in type 1 diabetes (T1D) pathogenesis and intervention practices. This study aimed to identify T1D-associated HLA gene alleles in the Omani population.METHODS: The present case-control study included 73 diabetic seropositive children (mean age 9.08 ± 3.27 years) attending the paediatric clinic at Sultan Qaboos University Hospital in Muscat, Oman, and 110 healthy controls.
HLA-A,
-B,
-C,
-DRB1 and
-DQB1 genes were genotyped using a sequence-specific primer polymerase chain reaction (SSP-PCR).
RESULTS: Two HLA class I alleles (
B*08,
B*58) and three class II alleles (
DQB1*02,
DRB1*03 and
DRB1*04) were associated with T1D susceptibility, while one class I (
B*51) and three class II (
DQB1*05,
DQB1*06 and
DRB1*16) alleles were associated with T1D protection.
HLA-DRB1*03 and
DQB1*02 alleles showed the strongest risk association among all alleles. Six
DRB1 residues (E
9, S
11, S
13, Y
30, V
70 and K
71) were significantly associated with T1D susceptibility. Heterozygous genotypes,
HLA-DRB1*03/
*04 and
DQB1*02/
*03 were significantly associated with T1D susceptibility (
P <0.0001, odds ratio [OR] = 63.21 and
P = 0.02, OR = 3.63, respectively). Furthermore, a significant combined action of
DRB1*03-
DQB1*02 haplotype in T1D risk (
P = 0.000176, OR = 15) and
DRB1*16-
DQB1*05 haplotype in protection (
P = 0.0312, OR = 0.48) was detected.
CONCLUSION: Known HLA class II gene alleles are associated with T1D in Omani children.
AB - OBJECTIVES: Identification of the high risk alleles, genotypes and haplotypes of the human leukocyte antigens (HLA) in different populations is beneficial for understanding their roles in type 1 diabetes (T1D) pathogenesis and intervention practices. This study aimed to identify T1D-associated HLA gene alleles in the Omani population.METHODS: The present case-control study included 73 diabetic seropositive children (mean age 9.08 ± 3.27 years) attending the paediatric clinic at Sultan Qaboos University Hospital in Muscat, Oman, and 110 healthy controls.
HLA-A,
-B,
-C,
-DRB1 and
-DQB1 genes were genotyped using a sequence-specific primer polymerase chain reaction (SSP-PCR).
RESULTS: Two HLA class I alleles (
B*08,
B*58) and three class II alleles (
DQB1*02,
DRB1*03 and
DRB1*04) were associated with T1D susceptibility, while one class I (
B*51) and three class II (
DQB1*05,
DQB1*06 and
DRB1*16) alleles were associated with T1D protection.
HLA-DRB1*03 and
DQB1*02 alleles showed the strongest risk association among all alleles. Six
DRB1 residues (E
9, S
11, S
13, Y
30, V
70 and K
71) were significantly associated with T1D susceptibility. Heterozygous genotypes,
HLA-DRB1*03/
*04 and
DQB1*02/
*03 were significantly associated with T1D susceptibility (
P <0.0001, odds ratio [OR] = 63.21 and
P = 0.02, OR = 3.63, respectively). Furthermore, a significant combined action of
DRB1*03-
DQB1*02 haplotype in T1D risk (
P = 0.000176, OR = 15) and
DRB1*16-
DQB1*05 haplotype in protection (
P = 0.0312, OR = 0.48) was detected.
CONCLUSION: Known HLA class II gene alleles are associated with T1D in Omani children.
KW - Ambulatory Care Facilities
KW - Case-Control Studies
KW - Child
KW - Child, Preschool
KW - Diabetes Mellitus, Type 1/genetics
KW - HLA-DRB1 Chains/genetics
KW - Hospitals, University
KW - Humans
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U2 - 10.18295/squmj.2.2022.016
DO - 10.18295/squmj.2.2022.016
M3 - Article
C2 - 36865417
SN - 2075-051X
VL - 23
SP - 68
EP - 75
JO - Sultan Qaboos University Medical Journal
JF - Sultan Qaboos University Medical Journal
IS - 1
ER -