The APETALA-2-like transcription factor OsAP2-39 controls key interactions between abscisic acid and gibberellin in rice

Mahmoud W. Yaish, Ashraf El-Kereamy, Tong Zhu, Perrin H. Beatty, Allen G. Good, Yong Mei Bi, Steven J. Rothstein

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

The interaction between phytohormones is an important mechanism which controls growth and developmental processes in plants. Deciphering these interactions is a crucial step in helping to develop crops with enhanced yield and resistance to environmental stresses. Controlling the expression level of OsAP2-39 which includes an APETALA 2 (AP2) domain leads to phenotypic changes in rice. Overexpression of OsAP2-39 leads to a reduction in yield by decreasing the biomass and the number of seeds in the transgenic rice lines. Global transcriptome analysis of the OsAP2-39 overexpression transgenic rice revealed the upregulation of a key Abscisic Acid (ABA) biosynthetic gene OsNCED-I which codes for 9-cis-epoxycarotenoid dioxygenase and leads to an increase in the endogenous ABA level. In addition to OsNCED-1, the gene expression analysis revealed the upregulation of a gene that codes for the Elongation of Upper most Internode (EUI) protein, an enzyme that catalyzes 16a, 17-epoxidation of non-13-hydroxylated GAs, which has been shown to deactivate gibberellins (GAs) in rice. The exogenous application of GA restores the wild-type phenotype in the transgenic line and ABA application induces the expression of EUI and suppresses the expression of OsAP2-39 in the wild-type line. These observations clarify the antagonistic relationship between ABA and GA and illustrate a mechanism that leads to homeostasis of these hormones. In vivo and in vitro analysis showed that the expression of both OsNCED-1 and EUI are directly controlled by OsAP2-39. Together, these results reveal a novel mechanism for the control of the ABA/GA balance in rice which is regulated by OsAP2- 39 that in turn regulates plant growth and seed production.

Original languageEnglish
Article numbere1001098
JournalPLoS Genetics
Volume6
Issue number9
DOIs
Publication statusPublished - Sep 2010

Fingerprint

Gibberellins
Abscisic Acid
abscisic acid
gibberellins
Transcription Factors
rice
transcription factors
internodes
9-cis-epoxy-carotenoid dioxygenase
genetically modified organisms
Seeds
Up-Regulation
seed productivity
Plant Growth Regulators
gene
homeostasis
Gene Expression Profiling
seed production
Growth
environmental stress

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Ecology, Evolution, Behavior and Systematics
  • Cancer Research
  • Genetics(clinical)

Cite this

The APETALA-2-like transcription factor OsAP2-39 controls key interactions between abscisic acid and gibberellin in rice. / Yaish, Mahmoud W.; El-Kereamy, Ashraf; Zhu, Tong; Beatty, Perrin H.; Good, Allen G.; Bi, Yong Mei; Rothstein, Steven J.

In: PLoS Genetics, Vol. 6, No. 9, e1001098, 09.2010.

Research output: Contribution to journalArticle

Yaish, Mahmoud W. ; El-Kereamy, Ashraf ; Zhu, Tong ; Beatty, Perrin H. ; Good, Allen G. ; Bi, Yong Mei ; Rothstein, Steven J. / The APETALA-2-like transcription factor OsAP2-39 controls key interactions between abscisic acid and gibberellin in rice. In: PLoS Genetics. 2010 ; Vol. 6, No. 9.
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