The anti-protozoal activity of bronopol on the key life-stages of Ichthyophthirius multifiliis Fouquet, 1876 (Ciliophora)

Andrew P. Shinn, Sara M. Picón-Camacho, James E. Bron, Denny Conway, Gil Ha Yoon, Fu Ci Guo, Nicholas G H Taylor

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Pyceze™ (Novartis Animal Vaccines Ltd.) is licensed as a veterinary medicine to treat fungal infections in salmon, trout and their eggs. The active ingredient is bronopol, which due to its broad-spectrum activity has the potential to be an effective treatment against other important aquatic pathogens. In this study the efficacy of bronopol against Ichthyophthirius multifiliis was tested both in vitro and in vivo. In vitro trials demonstrated a 30min exposure to 100mgL -1 bronopol killed 51.7% of the infective theronts. In vitro exposure of the protomonts to bronopol (0, 20, 50 and 100mgL -1) for 30min was observed to kill 0%, 76.2%, 97.2% and 100% respectively. Protomonts surviving treatment, demonstrated delayed development with the time taken from protomont until the release of theronts ranging from 28.3h for 0mgL -1 exposure, to 70h for parasites in 20 and 50mgL -1 exposure groups. These concentrations also caused asymmetric cell division of the encysted tomonts. Exposure of encysted tomonts (min. 8cell stage) to 100mgL -1 bronopol for 30min, killed 50% within this period, with the remainder dying within the subsequent 42h post exposure. Lower doses of bronopol were less effective in killing encysted tomonts than the higher doses (3.3% of parasites were killed in 20mgL -1; 10% in 50mgL -1), but they still delayed theront release significantly (25.7h for 0mgL -1 to 46.2h for parasites exposed to 20-50mgL -1).Long, low dose (1mgL -1) exposure to bronopol was also efficacious against theronts. Survival after 12h was 29% (c.f. 100% in control parasites), and

Original languageEnglish
Pages (from-to)229-236
Number of pages8
JournalVeterinary Parasitology
Volume186
Issue number3-4
DOIs
Publication statusPublished - May 25 2012

Fingerprint

Ciliophora
Ichthyophthirius multifiliis
parasites
dosage
fish roe
Parasites
active ingredients
trout
veterinary medicine
salmon
cell division
Asymmetric Cell Division
Communicable Disease Control
vaccines
Veterinary Medicine
Mycoses
Trout
Salmon
bronopol
pathogens

Keywords

  • Aquaculture
  • Bronopol
  • Management
  • Pyceze™
  • Treatment
  • White spot

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)

Cite this

The anti-protozoal activity of bronopol on the key life-stages of Ichthyophthirius multifiliis Fouquet, 1876 (Ciliophora). / Shinn, Andrew P.; Picón-Camacho, Sara M.; Bron, James E.; Conway, Denny; Yoon, Gil Ha; Guo, Fu Ci; Taylor, Nicholas G H.

In: Veterinary Parasitology, Vol. 186, No. 3-4, 25.05.2012, p. 229-236.

Research output: Contribution to journalArticle

Shinn, Andrew P. ; Picón-Camacho, Sara M. ; Bron, James E. ; Conway, Denny ; Yoon, Gil Ha ; Guo, Fu Ci ; Taylor, Nicholas G H. / The anti-protozoal activity of bronopol on the key life-stages of Ichthyophthirius multifiliis Fouquet, 1876 (Ciliophora). In: Veterinary Parasitology. 2012 ; Vol. 186, No. 3-4. pp. 229-236.
@article{b57ab7976c744616a231a20aca35ea5e,
title = "The anti-protozoal activity of bronopol on the key life-stages of Ichthyophthirius multifiliis Fouquet, 1876 (Ciliophora)",
abstract = "Pyceze™ (Novartis Animal Vaccines Ltd.) is licensed as a veterinary medicine to treat fungal infections in salmon, trout and their eggs. The active ingredient is bronopol, which due to its broad-spectrum activity has the potential to be an effective treatment against other important aquatic pathogens. In this study the efficacy of bronopol against Ichthyophthirius multifiliis was tested both in vitro and in vivo. In vitro trials demonstrated a 30min exposure to 100mgL -1 bronopol killed 51.7{\%} of the infective theronts. In vitro exposure of the protomonts to bronopol (0, 20, 50 and 100mgL -1) for 30min was observed to kill 0{\%}, 76.2{\%}, 97.2{\%} and 100{\%} respectively. Protomonts surviving treatment, demonstrated delayed development with the time taken from protomont until the release of theronts ranging from 28.3h for 0mgL -1 exposure, to 70h for parasites in 20 and 50mgL -1 exposure groups. These concentrations also caused asymmetric cell division of the encysted tomonts. Exposure of encysted tomonts (min. 8cell stage) to 100mgL -1 bronopol for 30min, killed 50{\%} within this period, with the remainder dying within the subsequent 42h post exposure. Lower doses of bronopol were less effective in killing encysted tomonts than the higher doses (3.3{\%} of parasites were killed in 20mgL -1; 10{\%} in 50mgL -1), but they still delayed theront release significantly (25.7h for 0mgL -1 to 46.2h for parasites exposed to 20-50mgL -1).Long, low dose (1mgL -1) exposure to bronopol was also efficacious against theronts. Survival after 12h was 29{\%} (c.f. 100{\%} in control parasites), and",
keywords = "Aquaculture, Bronopol, Management, Pyceze™, Treatment, White spot",
author = "Shinn, {Andrew P.} and Pic{\'o}n-Camacho, {Sara M.} and Bron, {James E.} and Denny Conway and Yoon, {Gil Ha} and Guo, {Fu Ci} and Taylor, {Nicholas G H}",
year = "2012",
month = "5",
day = "25",
doi = "10.1016/j.vetpar.2011.11.025",
language = "English",
volume = "186",
pages = "229--236",
journal = "Veterinary Parasitology: Regional Studies and Reports",
issn = "0304-4017",
publisher = "Elsevier",
number = "3-4",

}

TY - JOUR

T1 - The anti-protozoal activity of bronopol on the key life-stages of Ichthyophthirius multifiliis Fouquet, 1876 (Ciliophora)

AU - Shinn, Andrew P.

AU - Picón-Camacho, Sara M.

AU - Bron, James E.

AU - Conway, Denny

AU - Yoon, Gil Ha

AU - Guo, Fu Ci

AU - Taylor, Nicholas G H

PY - 2012/5/25

Y1 - 2012/5/25

N2 - Pyceze™ (Novartis Animal Vaccines Ltd.) is licensed as a veterinary medicine to treat fungal infections in salmon, trout and their eggs. The active ingredient is bronopol, which due to its broad-spectrum activity has the potential to be an effective treatment against other important aquatic pathogens. In this study the efficacy of bronopol against Ichthyophthirius multifiliis was tested both in vitro and in vivo. In vitro trials demonstrated a 30min exposure to 100mgL -1 bronopol killed 51.7% of the infective theronts. In vitro exposure of the protomonts to bronopol (0, 20, 50 and 100mgL -1) for 30min was observed to kill 0%, 76.2%, 97.2% and 100% respectively. Protomonts surviving treatment, demonstrated delayed development with the time taken from protomont until the release of theronts ranging from 28.3h for 0mgL -1 exposure, to 70h for parasites in 20 and 50mgL -1 exposure groups. These concentrations also caused asymmetric cell division of the encysted tomonts. Exposure of encysted tomonts (min. 8cell stage) to 100mgL -1 bronopol for 30min, killed 50% within this period, with the remainder dying within the subsequent 42h post exposure. Lower doses of bronopol were less effective in killing encysted tomonts than the higher doses (3.3% of parasites were killed in 20mgL -1; 10% in 50mgL -1), but they still delayed theront release significantly (25.7h for 0mgL -1 to 46.2h for parasites exposed to 20-50mgL -1).Long, low dose (1mgL -1) exposure to bronopol was also efficacious against theronts. Survival after 12h was 29% (c.f. 100% in control parasites), and

AB - Pyceze™ (Novartis Animal Vaccines Ltd.) is licensed as a veterinary medicine to treat fungal infections in salmon, trout and their eggs. The active ingredient is bronopol, which due to its broad-spectrum activity has the potential to be an effective treatment against other important aquatic pathogens. In this study the efficacy of bronopol against Ichthyophthirius multifiliis was tested both in vitro and in vivo. In vitro trials demonstrated a 30min exposure to 100mgL -1 bronopol killed 51.7% of the infective theronts. In vitro exposure of the protomonts to bronopol (0, 20, 50 and 100mgL -1) for 30min was observed to kill 0%, 76.2%, 97.2% and 100% respectively. Protomonts surviving treatment, demonstrated delayed development with the time taken from protomont until the release of theronts ranging from 28.3h for 0mgL -1 exposure, to 70h for parasites in 20 and 50mgL -1 exposure groups. These concentrations also caused asymmetric cell division of the encysted tomonts. Exposure of encysted tomonts (min. 8cell stage) to 100mgL -1 bronopol for 30min, killed 50% within this period, with the remainder dying within the subsequent 42h post exposure. Lower doses of bronopol were less effective in killing encysted tomonts than the higher doses (3.3% of parasites were killed in 20mgL -1; 10% in 50mgL -1), but they still delayed theront release significantly (25.7h for 0mgL -1 to 46.2h for parasites exposed to 20-50mgL -1).Long, low dose (1mgL -1) exposure to bronopol was also efficacious against theronts. Survival after 12h was 29% (c.f. 100% in control parasites), and

KW - Aquaculture

KW - Bronopol

KW - Management

KW - Pyceze™

KW - Treatment

KW - White spot

UR - http://www.scopus.com/inward/record.url?scp=84858079344&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84858079344&partnerID=8YFLogxK

U2 - 10.1016/j.vetpar.2011.11.025

DO - 10.1016/j.vetpar.2011.11.025

M3 - Article

VL - 186

SP - 229

EP - 236

JO - Veterinary Parasitology: Regional Studies and Reports

JF - Veterinary Parasitology: Regional Studies and Reports

SN - 0304-4017

IS - 3-4

ER -