TGF-β2: A novel target of CD44-promoted breast cancer invasion

Allal Ouhtit*, Samineh Madani, Ishita Gupta, Somya Shanmuganathan, Mohamed E. Abdraboh, Hamad Al-Riyami, Yahya M. Al-Farsi, Madhwa H G Raj

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

We have developed a tetracycline (tet)-off regulated expression of CD44s gene in the breast cancer (BC) cell line MCF-7 (B5 clone) and identified TGF-β2 (Transforming Growth Factor beta- 2; 3 fold induction) as a potential CD44-downstream transcriptional target by microarray analysis. To further validate this finding, the same RNA samples, used for microarray analysis and their corresponding protein lysates, collected from the BC cell line MCF-7-B5, were examined for CD44 expression in the presence of HA. Our results showed that TGF-β2 mRNA levels were significantly elevated following theremoval of tetracycline at 18, 24, and 48 h post-HA stimulation compared to the parental cells. Furthermore, the TGF-β2 precursor protein increased in a time-dependent pattern upon HA-stimulation and in the absence of tetracycline. More interestingly, inhibition of CD44 gene by RNAi method decreased TGF-β2 expression upon HA-stimulation, and subsequently inhibited BC cell invasion in vitro. In addition to identifying TGF-β2 as a target for HA/CD44 signaling, this data suggests that ATF/CREB might be a potential transcription factor linking HA/CD44 activation to TGF-β2 transcription and additional experiments are required for a better understanding of the molecular mechanisms underpinning the novel function of the CD44/ TGF-β2 signaling pathway in breast cancer metastasis.

Original languageEnglish
Pages (from-to)566-572
Number of pages7
JournalJournal of Cancer
Volume4
Issue number7
DOIs
Publication statusPublished - 2013

Keywords

  • Breast cancer
  • Cd44
  • Cre/Atf
  • Hyaluronan
  • Mcf-7-B5 Clone
  • Metastasis
  • Tetracycline-Inducible
  • Tgf-β2

ASJC Scopus subject areas

  • Oncology

Fingerprint Dive into the research topics of 'TGF-β2: A novel target of CD44-promoted breast cancer invasion'. Together they form a unique fingerprint.

Cite this