Systemic induction of pathogenesis related proteins (PRs) in Alternaria solani elicitor sensitized tomato cells as resistance response

R. Radhajeyalakshmi, R. Velazhahan, R. Samiyappan, Sabitha Doraiswamy

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Induction of acquired resistance and pathogenesis related proteins in intact plant leaves and the potentiation of pathogen signals in plant cell cultures by elicitors is mediated by different signaling pathways. We show here, the induction of PR proteins, the most widely used marker of acquired resistance, in tomato cells in response to Alternaria solani elicitors. The crude glycoprotein elicitor preparation from A. solani was used to detect the possible induction of pathogenesis - related proteins chitinase (PR-3), thaumatin like proteins (PR-5) and β- 1,3 glucanase in suspension - cultured cells and leaves of tomato cultivar CO 3. Western blot analysis revealed the presence of 23 kDa thaumatin like protein and 44 kDa chitinase in elicitor treated suspension - cultured cells and leaves of tomato. The biochemical approach described in this paper with tomato should provide the basis for further efforts concentrating on the isolation and characterization of elements involved in perception and in the early steps of intracellular signal transduction.

Original languageEnglish
Pages (from-to)685-689
Number of pages5
JournalScientific Research and Essays
Volume4
Issue number7
Publication statusPublished - Jul 2009

Fingerprint

tomatoes
Alternaria solani
Alternaria
pathogenesis
pathogenesis-related proteins
Lycopersicon esculentum
induction
proteins
Proteins
cells
leaves
chitinase
Chitinases
cultured cells
Cultured Cells
Suspensions
Plant cell culture
Cells
concentrating
Signal transduction

Keywords

  • Alternaria solani
  • Elicitors
  • Pathogenesis - related proteins
  • Suspension-culture
  • Tomato

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Systemic induction of pathogenesis related proteins (PRs) in Alternaria solani elicitor sensitized tomato cells as resistance response. / Radhajeyalakshmi, R.; Velazhahan, R.; Samiyappan, R.; Doraiswamy, Sabitha.

In: Scientific Research and Essays, Vol. 4, No. 7, 07.2009, p. 685-689.

Research output: Contribution to journalArticle

@article{592f9b3679814e2c8720dd2f0dac8db6,
title = "Systemic induction of pathogenesis related proteins (PRs) in Alternaria solani elicitor sensitized tomato cells as resistance response",
abstract = "Induction of acquired resistance and pathogenesis related proteins in intact plant leaves and the potentiation of pathogen signals in plant cell cultures by elicitors is mediated by different signaling pathways. We show here, the induction of PR proteins, the most widely used marker of acquired resistance, in tomato cells in response to Alternaria solani elicitors. The crude glycoprotein elicitor preparation from A. solani was used to detect the possible induction of pathogenesis - related proteins chitinase (PR-3), thaumatin like proteins (PR-5) and β- 1,3 glucanase in suspension - cultured cells and leaves of tomato cultivar CO 3. Western blot analysis revealed the presence of 23 kDa thaumatin like protein and 44 kDa chitinase in elicitor treated suspension - cultured cells and leaves of tomato. The biochemical approach described in this paper with tomato should provide the basis for further efforts concentrating on the isolation and characterization of elements involved in perception and in the early steps of intracellular signal transduction.",
keywords = "Alternaria solani, Elicitors, Pathogenesis - related proteins, Suspension-culture, Tomato",
author = "R. Radhajeyalakshmi and R. Velazhahan and R. Samiyappan and Sabitha Doraiswamy",
year = "2009",
month = "7",
language = "English",
volume = "4",
pages = "685--689",
journal = "Scientific Research and Essays",
issn = "1992-2248",
publisher = "Academic Journals",
number = "7",

}

TY - JOUR

T1 - Systemic induction of pathogenesis related proteins (PRs) in Alternaria solani elicitor sensitized tomato cells as resistance response

AU - Radhajeyalakshmi, R.

AU - Velazhahan, R.

AU - Samiyappan, R.

AU - Doraiswamy, Sabitha

PY - 2009/7

Y1 - 2009/7

N2 - Induction of acquired resistance and pathogenesis related proteins in intact plant leaves and the potentiation of pathogen signals in plant cell cultures by elicitors is mediated by different signaling pathways. We show here, the induction of PR proteins, the most widely used marker of acquired resistance, in tomato cells in response to Alternaria solani elicitors. The crude glycoprotein elicitor preparation from A. solani was used to detect the possible induction of pathogenesis - related proteins chitinase (PR-3), thaumatin like proteins (PR-5) and β- 1,3 glucanase in suspension - cultured cells and leaves of tomato cultivar CO 3. Western blot analysis revealed the presence of 23 kDa thaumatin like protein and 44 kDa chitinase in elicitor treated suspension - cultured cells and leaves of tomato. The biochemical approach described in this paper with tomato should provide the basis for further efforts concentrating on the isolation and characterization of elements involved in perception and in the early steps of intracellular signal transduction.

AB - Induction of acquired resistance and pathogenesis related proteins in intact plant leaves and the potentiation of pathogen signals in plant cell cultures by elicitors is mediated by different signaling pathways. We show here, the induction of PR proteins, the most widely used marker of acquired resistance, in tomato cells in response to Alternaria solani elicitors. The crude glycoprotein elicitor preparation from A. solani was used to detect the possible induction of pathogenesis - related proteins chitinase (PR-3), thaumatin like proteins (PR-5) and β- 1,3 glucanase in suspension - cultured cells and leaves of tomato cultivar CO 3. Western blot analysis revealed the presence of 23 kDa thaumatin like protein and 44 kDa chitinase in elicitor treated suspension - cultured cells and leaves of tomato. The biochemical approach described in this paper with tomato should provide the basis for further efforts concentrating on the isolation and characterization of elements involved in perception and in the early steps of intracellular signal transduction.

KW - Alternaria solani

KW - Elicitors

KW - Pathogenesis - related proteins

KW - Suspension-culture

KW - Tomato

UR - http://www.scopus.com/inward/record.url?scp=70349102450&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349102450&partnerID=8YFLogxK

M3 - Article

VL - 4

SP - 685

EP - 689

JO - Scientific Research and Essays

JF - Scientific Research and Essays

SN - 1992-2248

IS - 7

ER -