Sublethal effects of esfenvalerate pulse-exposure on spawning and non-spawning Australian crimson-spotted rainbowfish (Melanotaenia fluviatilis)

M. J. Barry, K. O'Halloran, D. C. Logan, J. T. Ahokas, D. A. Holdway

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The spawning activity of the Australian crimson-spotted rainbowfish (Melanotaenia fluviatilis) was monitored over a period of nine days. After the first three days, groups of rainbowfish were pulse-exposed with the pyrethroid insecticide esfenvalerate at one of six nominal concentrations (0, 1, 3.2, 10.0, 32.0, or 100 μ/L). The effects of esfenvalerate on fecundity, total hatch, hatchability of eggs, frequency of hatch defects, and the survival and growth of subsequent larvae were monitored. Hepatic cytochrome P-450 activity (ECOD, EROD, EFCOD) was measured six days post-exposure in controls and fish exposed to 32 μg/L esfenvalerate. Cytochrome P-450 activity was also measured in single sex populations of male and female rainbowfish similarly exposed. The effects of esfenvalerate pulse-exposure on the lymphoproliferation of immune cells isolated from spawning and non-spawning groups of rainbowfish were examined in a second experiment. Over 75% of fish pulse-exposed to 100 μg/L esfenvalerate died, and males in all treatments were more strongly affected than females. There was a negative correlation between pesticide concentration, fecundity, and total hatch. All eggs laid in the first three days post-exposure failed to hatch in some treatments, although esfenvalerate pulse-exposure did not increase the prevalence of deformed larvae. Esfenvalerate pulse-exposure significantly increased hepatic ECOD activity in male rainbowfish but had no effect on male EROD or EFCOD activities. There was no significant effect of esfenvalerate pulse-exposure on hepatic EROD, ECOD, or EFCOD activities of female rainbowfish. Unstimulated lymphocyte proliferation was lower in treated female rainbowfish than non-exposed female fish, and there was some evidence of an interaction with spawning activity.

Original languageEnglish
Pages (from-to)459-463
Number of pages5
JournalArchives of Environmental Contamination and Toxicology
Volume28
Issue number4
DOIs
Publication statusPublished - May 1995

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fenvalerate
Hatches
sublethal effect
spawning
Cytochrome P-450 CYP1A1
Fish
Fishes
Insecticides
cytochrome
Lymphocytes
fecundity
fish
Pesticides
Cytochrome P-450 Enzyme System
Eggs
egg
larva
Fertility
Larva
Liver

ASJC Scopus subject areas

  • Toxicology
  • Environmental Chemistry
  • Environmental Science(all)

Cite this

Sublethal effects of esfenvalerate pulse-exposure on spawning and non-spawning Australian crimson-spotted rainbowfish (Melanotaenia fluviatilis). / Barry, M. J.; O'Halloran, K.; Logan, D. C.; Ahokas, J. T.; Holdway, D. A.

In: Archives of Environmental Contamination and Toxicology, Vol. 28, No. 4, 05.1995, p. 459-463.

Research output: Contribution to journalArticle

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abstract = "The spawning activity of the Australian crimson-spotted rainbowfish (Melanotaenia fluviatilis) was monitored over a period of nine days. After the first three days, groups of rainbowfish were pulse-exposed with the pyrethroid insecticide esfenvalerate at one of six nominal concentrations (0, 1, 3.2, 10.0, 32.0, or 100 μ/L). The effects of esfenvalerate on fecundity, total hatch, hatchability of eggs, frequency of hatch defects, and the survival and growth of subsequent larvae were monitored. Hepatic cytochrome P-450 activity (ECOD, EROD, EFCOD) was measured six days post-exposure in controls and fish exposed to 32 μg/L esfenvalerate. Cytochrome P-450 activity was also measured in single sex populations of male and female rainbowfish similarly exposed. The effects of esfenvalerate pulse-exposure on the lymphoproliferation of immune cells isolated from spawning and non-spawning groups of rainbowfish were examined in a second experiment. Over 75{\%} of fish pulse-exposed to 100 μg/L esfenvalerate died, and males in all treatments were more strongly affected than females. There was a negative correlation between pesticide concentration, fecundity, and total hatch. All eggs laid in the first three days post-exposure failed to hatch in some treatments, although esfenvalerate pulse-exposure did not increase the prevalence of deformed larvae. Esfenvalerate pulse-exposure significantly increased hepatic ECOD activity in male rainbowfish but had no effect on male EROD or EFCOD activities. There was no significant effect of esfenvalerate pulse-exposure on hepatic EROD, ECOD, or EFCOD activities of female rainbowfish. Unstimulated lymphocyte proliferation was lower in treated female rainbowfish than non-exposed female fish, and there was some evidence of an interaction with spawning activity.",
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