TY - JOUR
T1 - Streptomyces lividans and Brevibacterium lactofermentum as heterologous hosts for the production of X22 xylanase from Aspergillus nidulans
AU - Díaz, M.
AU - Adham, S. A.I.
AU - Ramón, D.
AU - Gil, J. A.
AU - Santamaría, R. I.
N1 - Funding Information:
Acknowledgements This research was supported by a grant from the European Union (FD1997-1134-C03). We thank R. Valle for her excellent technical work. Drs. J.M. Férnandez-Abalos and F. Leal are thanked for their comments. Thanks are also due to N. Skinner for supervising the English version of the manuscript. These experiments comply with the current laws in Spain.
PY - 2004/9
Y1 - 2004/9
N2 - The Aspergillus nidulans gene xlnA coding for the fungal xylanase X 22 has been cloned and expressed in two heterologous bacterial hosts: Streptomyces lividans and Brevibacterium lactofermentum. Streptomyces strains yielded 10 units/ml of xylanase when the protein was produced with its own signal peptide, and 19 units/ml when its signal peptide was replaced by the one for xylanase Xys1 from Streptomyces halstedii. B. lactofermentum was also able to produce xylanase X22, affording 6 units/ml upon using either the Aspergillus xlnA signal peptide or Streptomyces xysA. These production values are higher than those previously reported for the heterologous expression of the A. nidulans xlnA gene in Saccharomyces cerevisiae (1 unit/ml). Moreover, the X22 enzyme produced by Streptomyces lividans showed oenological properties, indicating that this Streptomyces recombinant strain is a good candidate for the production of this enzyme at the industrial scale.
AB - The Aspergillus nidulans gene xlnA coding for the fungal xylanase X 22 has been cloned and expressed in two heterologous bacterial hosts: Streptomyces lividans and Brevibacterium lactofermentum. Streptomyces strains yielded 10 units/ml of xylanase when the protein was produced with its own signal peptide, and 19 units/ml when its signal peptide was replaced by the one for xylanase Xys1 from Streptomyces halstedii. B. lactofermentum was also able to produce xylanase X22, affording 6 units/ml upon using either the Aspergillus xlnA signal peptide or Streptomyces xysA. These production values are higher than those previously reported for the heterologous expression of the A. nidulans xlnA gene in Saccharomyces cerevisiae (1 unit/ml). Moreover, the X22 enzyme produced by Streptomyces lividans showed oenological properties, indicating that this Streptomyces recombinant strain is a good candidate for the production of this enzyme at the industrial scale.
UR - http://www.scopus.com/inward/record.url?scp=4644347306&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=4644347306&partnerID=8YFLogxK
U2 - 10.1007/s00253-004-1633-3
DO - 10.1007/s00253-004-1633-3
M3 - Article
C2 - 15168093
AN - SCOPUS:4644347306
SN - 0175-7598
VL - 65
SP - 401
EP - 406
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
IS - 4
ER -