Streptomyces lividans and Brevibacterium lactofermentum as heterologous hosts for the production of X22 xylanase from Aspergillus nidulans

M. Díaz, S. A I Adham, D. Ramón, J. A. Gil, R. I. Santamaría

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The Aspergillus nidulans gene xlnA coding for the fungal xylanase X 22 has been cloned and expressed in two heterologous bacterial hosts: Streptomyces lividans and Brevibacterium lactofermentum. Streptomyces strains yielded 10 units/ml of xylanase when the protein was produced with its own signal peptide, and 19 units/ml when its signal peptide was replaced by the one for xylanase Xys1 from Streptomyces halstedii. B. lactofermentum was also able to produce xylanase X22, affording 6 units/ml upon using either the Aspergillus xlnA signal peptide or Streptomyces xysA. These production values are higher than those previously reported for the heterologous expression of the A. nidulans xlnA gene in Saccharomyces cerevisiae (1 unit/ml). Moreover, the X22 enzyme produced by Streptomyces lividans showed oenological properties, indicating that this Streptomyces recombinant strain is a good candidate for the production of this enzyme at the industrial scale.

Original languageEnglish
Pages (from-to)401-406
Number of pages6
JournalApplied Microbiology and Biotechnology
Volume65
Issue number4
DOIs
Publication statusPublished - Sep 2004

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology
  • Bioengineering

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