Some physiological and histological aspects of the gastrointestinal tract in a mouse model of chronic renal failure

B. H. Ali, T. T. Madanagopal, A. Ramkumar, A. Boudaka, M. H. Tageldin, A. Nemmar

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Introduction: It has been reported that mice with 5/6 nephrectomy- induced chronic renal failure (CRF) have reduced gastrointestinal transit (GIT) and increased fecal moisture content (FMC). We have recently shown that feeding adenine (0.2%, w/w) to mice can be used as a model of CRF. Here, we investigated the possible effects of adenine-induced CRF on several in vivo and in vitro aspects of GIT physiology and histology of the stomach, duodenum, ileum and colon in mice. Methods: The effects of CRF induced by feeding adenine (0.2%, w/w for 2 or 4. weeks) on the gastric emptying index (GEI), GIT, FMC and bead expulsion test (BET) were investigated. GIT was measured by the charcoal meal test and GEI by the difference between full and empty stomach weights. Fresh and dried feces were weighed to calculate the FMC. Renal function was assessed histologically, and biochemically in plasma and urine. The light microscopic histology of the different parts of the gut, as well as the in vitro contractility of the isolated ileum was also assessed. Results: Feeding adenine for 2 or 4. weeks resulted in CRF. The BET was significantly increased in mice given adenine for 2 but not 4. weeks, while the GEI was significantly increased in mice treated with adenine for 4 but not 2. weeks. No significant differences between control and adenine-treated mice were found in GIT, FMC or the histology of the different parts of the gut. Acetylcholine-induced contractions of the ileum of adenine-treated rats were not significantly different from those of the controls. Discussion: Feeding adenine for either 2 or 4. weeks resulted in CRF, but it would appear that this model produces effects on the gastrointestinal tract that are milder than those reported before in animal models with 5/6 nephrectomy-induced-CRF.

Original languageEnglish
Pages (from-to)162-166
Number of pages5
JournalJournal of Pharmacological and Toxicological Methods
Volume69
Issue number2
DOIs
Publication statusPublished - 2014

Fingerprint

Adenine
Chronic Kidney Failure
Gastrointestinal Tract
Gastrointestinal Transit
Histology
Gastric Emptying
Moisture
Ileum
Nephrectomy
Stomach
Charcoal
Physiology
Duodenum
Feces
Acetylcholine
Meals
Rats
Colon
Animals
Animal Models

Keywords

  • Adenine
  • Chronic renal failure
  • Gastric emptying transit time
  • Gastrointestinal tract
  • Mice

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

Cite this

Some physiological and histological aspects of the gastrointestinal tract in a mouse model of chronic renal failure. / Ali, B. H.; Madanagopal, T. T.; Ramkumar, A.; Boudaka, A.; Tageldin, M. H.; Nemmar, A.

In: Journal of Pharmacological and Toxicological Methods, Vol. 69, No. 2, 2014, p. 162-166.

Research output: Contribution to journalArticle

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AU - Madanagopal, T. T.

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AU - Tageldin, M. H.

AU - Nemmar, A.

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