Simultaneous determination of total phenolic acids and total flavonoids in tea and honey samples using an integrated lab on a chip device

Nafiseh Bagheri, Haider A.J. Al Lawati*, Javad Hassanzadeh

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


A microfluidic device for simultaneous analysis of total flavonoids and total phenolic acids is presented for the first time. The process was based on the utilization of magnetic zinc-imidazole frameworks (ZIF-4), as a highly efficient media for on-line separation of phenolic acids and flavonoids, which were subsequently detected by a sensitive chemiluminescence (CL) method. Acrylate-based polymeric microchips containing a separation column (12.5 mm length, 3 mm width and 1 mm depth) were fabricated using a 3D-printer, and magnetic ZIF-4 was fixed into the column. The high porosity of the magnetic ZIF-4 made it a suitable adsorbent, guaranteeing an effective separation. The detection limits were in the range of 0.04–0.10 µg mL−1 with relative standard deviation values of (2.19–4.38%). The system was successfully applied for the analysis of flavonoids and phenolic acids in tea and honey samples. The recovery was from 95.4 to 104.1%, indicating a good accuracy of the developed method.

Original languageEnglish
Article number128338
JournalFood Chemistry
Publication statusPublished - Apr 16 2021
Externally publishedYes


  • Chemiluminescence
  • Flavonoids
  • Microfluidic chip
  • On-line separation
  • Total phenolic compounds

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science

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