Abstract
The modified simplex method was applied to the selection of the proper experimental conditions for the flow injection spectrophotometric determination of tetracycline, chlortetracycline, oxytetracycline and demeclocycline. In the method a 157 mm3 sample volume was injected, for all the compounds, into the carrier stream of iron(III) of concentrations 554, 626, 701 and 447 ppm flowing at rates of 3.72, 4.37, 3.72 and 3.72 cm3 min-1, thus passing through a reaction coil of length 55,85,45 and 45 cm for the respective compounds, all in 0.001 mol dm-3 sulfuric acid as an over-all reaction medium. A high sampling frequency of the order of at least 170 h-1 was attained for all the compounds. A high precision with a relative standard deviation of less than 0.9% (n = 5) was also obtained. The accuracy was found to be high as the Student t-values were calculated to be less than the theoretical values when the results were compared with those obtained by the conventional spectrophotometric method. There were no interferences from excipients in dosage forms when the method was applied to pharmaceutical preparations.
| Original language | English |
|---|---|
| Pages (from-to) | 1179-1183 |
| Number of pages | 5 |
| Journal | Analyst |
| Volume | 117 |
| Issue number | 7 |
| Publication status | Published - 1992 |
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Keywords
- Drug formulations
- Flow injection
- Simplex method
- Spectrophotometry
- Tetracycline antibiotics
ASJC Scopus subject areas
- Analytical Chemistry
Cite this
Simplex-optimized and flow injection spectrophotometric assay of tetracycline antibiotics in drug formulations. / Sultan, Salah M.; Suliman, Fakhr Eldin O; Duffuaa, Salih O.; Abu-Abdoun, Ideisan I.
In: Analyst, Vol. 117, No. 7, 1992, p. 1179-1183.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Simplex-optimized and flow injection spectrophotometric assay of tetracycline antibiotics in drug formulations
AU - Sultan, Salah M.
AU - Suliman, Fakhr Eldin O
AU - Duffuaa, Salih O.
AU - Abu-Abdoun, Ideisan I.
PY - 1992
Y1 - 1992
N2 - The modified simplex method was applied to the selection of the proper experimental conditions for the flow injection spectrophotometric determination of tetracycline, chlortetracycline, oxytetracycline and demeclocycline. In the method a 157 mm3 sample volume was injected, for all the compounds, into the carrier stream of iron(III) of concentrations 554, 626, 701 and 447 ppm flowing at rates of 3.72, 4.37, 3.72 and 3.72 cm3 min-1, thus passing through a reaction coil of length 55,85,45 and 45 cm for the respective compounds, all in 0.001 mol dm-3 sulfuric acid as an over-all reaction medium. A high sampling frequency of the order of at least 170 h-1 was attained for all the compounds. A high precision with a relative standard deviation of less than 0.9% (n = 5) was also obtained. The accuracy was found to be high as the Student t-values were calculated to be less than the theoretical values when the results were compared with those obtained by the conventional spectrophotometric method. There were no interferences from excipients in dosage forms when the method was applied to pharmaceutical preparations.
AB - The modified simplex method was applied to the selection of the proper experimental conditions for the flow injection spectrophotometric determination of tetracycline, chlortetracycline, oxytetracycline and demeclocycline. In the method a 157 mm3 sample volume was injected, for all the compounds, into the carrier stream of iron(III) of concentrations 554, 626, 701 and 447 ppm flowing at rates of 3.72, 4.37, 3.72 and 3.72 cm3 min-1, thus passing through a reaction coil of length 55,85,45 and 45 cm for the respective compounds, all in 0.001 mol dm-3 sulfuric acid as an over-all reaction medium. A high sampling frequency of the order of at least 170 h-1 was attained for all the compounds. A high precision with a relative standard deviation of less than 0.9% (n = 5) was also obtained. The accuracy was found to be high as the Student t-values were calculated to be less than the theoretical values when the results were compared with those obtained by the conventional spectrophotometric method. There were no interferences from excipients in dosage forms when the method was applied to pharmaceutical preparations.
KW - Drug formulations
KW - Flow injection
KW - Simplex method
KW - Spectrophotometry
KW - Tetracycline antibiotics
UR - http://www.scopus.com/inward/record.url?scp=0026721950&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026721950&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0026721950
VL - 117
SP - 1179
EP - 1183
JO - The Analyst
JF - The Analyst
SN - 0003-2654
IS - 7
ER -